Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_665 and Pf6N2E2_666 are separated by 36 nucleotides Pf6N2E2_666 and Pf6N2E2_667 overlap by 1 nucleotides
Pf6N2E2_665 - Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-), at 770,028 to 771,005
_665
Pf6N2E2_666 - Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-), at 771,042 to 772,058
_666
Pf6N2E2_667 - Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-), at 772,058 to 773,170
_667
Position (kb) 771
772
773 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 770.155 kb on - strand, within Pf6N2E2_665 at 770.155 kb on - strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.290 kb on - strand, within Pf6N2E2_665 at 770.560 kb on - strand, within Pf6N2E2_665 at 770.934 kb on + strand at 770.988 kb on + strand at 770.989 kb on - strand at 770.998 kb on - strand at 771.028 kb on + strand at 771.028 kb on + strand at 771.029 kb on - strand at 771.029 kb on - strand at 771.378 kb on + strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.526 kb on - strand, within Pf6N2E2_666 at 771.601 kb on - strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.776 kb on - strand, within Pf6N2E2_666 at 771.884 kb on + strand, within Pf6N2E2_666 at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 772.072 kb on + strand at 772.782 kb on - strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.895 kb on + strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.896 kb on - strand, within Pf6N2E2_667 at 772.948 kb on - strand, within Pf6N2E2_667 at 772.989 kb on + strand, within Pf6N2E2_667 at 772.990 kb on - strand, within Pf6N2E2_667
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 770,155 - Pf6N2E2_665 0.13 -1.0 770,155 - Pf6N2E2_665 0.13 +0.3 770,289 + Pf6N2E2_665 0.27 -0.6 770,289 + Pf6N2E2_665 0.27 +0.1 770,290 - Pf6N2E2_665 0.27 -0.2 770,560 - Pf6N2E2_665 0.54 -0.3 770,934 + +0.5 770,988 + +0.6 770,989 - +0.6 770,998 - -0.6 771,028 + -0.5 771,028 + -0.4 771,029 - +1.0 771,029 - -0.5 771,378 + Pf6N2E2_666 0.33 +0.1 771,379 - Pf6N2E2_666 0.33 -1.0 771,379 - Pf6N2E2_666 0.33 +0.1 771,506 - Pf6N2E2_666 0.46 -1.6 771,506 - Pf6N2E2_666 0.46 -0.2 771,526 - Pf6N2E2_666 0.48 -1.2 771,601 - Pf6N2E2_666 0.55 -0.5 771,633 + Pf6N2E2_666 0.58 -0.9 771,633 + Pf6N2E2_666 0.58 -1.2 771,776 - Pf6N2E2_666 0.72 +0.2 771,884 + Pf6N2E2_666 0.83 +0.2 771,993 + -0.7 771,993 + -1.0 771,993 + +1.4 771,993 + +0.2 771,993 + -0.3 771,994 - +2.8 771,994 - +0.5 771,994 - +0.7 771,994 - -1.8 771,994 - -0.0 772,072 + -0.6 772,782 - Pf6N2E2_667 0.65 -0.0 772,895 + Pf6N2E2_667 0.75 -1.0 772,895 + Pf6N2E2_667 0.75 -0.3 772,896 - Pf6N2E2_667 0.75 +0.0 772,896 - Pf6N2E2_667 0.75 -0.9 772,948 - Pf6N2E2_667 0.80 +1.1 772,989 + Pf6N2E2_667 0.84 +0.0 772,990 - Pf6N2E2_667 0.84 -0.5
Or see this region's nucleotide sequence