Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_661

Experiment: thiosulfate 0.25 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_659 and Pf6N2E2_660 are separated by 223 nucleotidesPf6N2E2_660 and Pf6N2E2_661 are separated by 168 nucleotidesPf6N2E2_661 and Pf6N2E2_662 are separated by 242 nucleotidesPf6N2E2_662 and Pf6N2E2_663 are separated by 4 nucleotides Pf6N2E2_659 - Transcriptional regulatory protein ompR, at 763,572 to 764,312 _659 Pf6N2E2_660 - hypothetical protein, at 764,536 to 764,799 _660 Pf6N2E2_661 - DipZ protein, at 764,968 to 766,179 _661 Pf6N2E2_662 - Valyl-tRNA synthetase, at 766,422 to 766,739 _662 Pf6N2E2_663 - Transcriptional activator of acetoin dehydrogenase operon AcoR, at 766,744 to 768,609 _663 Position (kb) 764 765 766 767Strain fitness (log2 ratio) -2 -1 0 1 2at 764.006 kb on - strand, within Pf6N2E2_659at 764.006 kb on - strand, within Pf6N2E2_659at 764.074 kb on - strand, within Pf6N2E2_659at 764.246 kb on - strandat 764.286 kb on + strandat 764.287 kb on - strandat 764.287 kb on - strandat 764.349 kb on + strandat 764.384 kb on + strandat 764.385 kb on - strandat 764.385 kb on - strandat 764.385 kb on - strandat 764.385 kb on - strandat 764.394 kb on + strandat 764.395 kb on - strandat 764.510 kb on + strandat 764.511 kb on - strandat 764.554 kb on + strandat 764.555 kb on - strandat 764.632 kb on + strand, within Pf6N2E2_660at 764.632 kb on + strand, within Pf6N2E2_660at 764.633 kb on - strand, within Pf6N2E2_660at 764.725 kb on + strand, within Pf6N2E2_660at 764.726 kb on - strand, within Pf6N2E2_660at 764.938 kb on + strandat 765.682 kb on + strand, within Pf6N2E2_661at 765.682 kb on + strand, within Pf6N2E2_661at 765.682 kb on + strand, within Pf6N2E2_661at 765.683 kb on - strand, within Pf6N2E2_661at 765.683 kb on - strand, within Pf6N2E2_661at 765.683 kb on - strand, within Pf6N2E2_661at 765.683 kb on - strand, within Pf6N2E2_661at 765.835 kb on + strand, within Pf6N2E2_661at 765.835 kb on + strand, within Pf6N2E2_661at 765.836 kb on - strand, within Pf6N2E2_661at 765.868 kb on + strand, within Pf6N2E2_661at 765.869 kb on - strand, within Pf6N2E2_661at 765.937 kb on + strand, within Pf6N2E2_661at 765.977 kb on + strand, within Pf6N2E2_661at 765.998 kb on - strand, within Pf6N2E2_661at 766.051 kb on + strand, within Pf6N2E2_661at 766.066 kb on + strandat 766.067 kb on - strandat 766.321 kb on + strandat 766.570 kb on - strand, within Pf6N2E2_662at 766.653 kb on + strand, within Pf6N2E2_662at 766.653 kb on + strand, within Pf6N2E2_662at 766.654 kb on - strand, within Pf6N2E2_662at 766.654 kb on - strand, within Pf6N2E2_662at 766.654 kb on - strand, within Pf6N2E2_662at 766.698 kb on + strand, within Pf6N2E2_662at 766.698 kb on + strand, within Pf6N2E2_662at 766.698 kb on + strand, within Pf6N2E2_662at 766.699 kb on - strand, within Pf6N2E2_662at 766.701 kb on + strand, within Pf6N2E2_662at 766.701 kb on + strand, within Pf6N2E2_662at 766.702 kb on - strand, within Pf6N2E2_662at 766.702 kb on - strand, within Pf6N2E2_662at 766.738 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction thiosulfate 0.25 M
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764,006 - Pf6N2E2_659 0.59 -0.6
764,006 - Pf6N2E2_659 0.59 +0.8
764,074 - Pf6N2E2_659 0.68 -0.4
764,246 - +1.1
764,286 + +0.2
764,287 - +1.0
764,287 - -0.6
764,349 + -1.5
764,384 + +0.6
764,385 - +0.4
764,385 - +0.6
764,385 - +0.4
764,385 - +1.2
764,394 + +0.1
764,395 - -0.9
764,510 + -0.2
764,511 - -0.5
764,554 + +1.0
764,555 - -0.4
764,632 + Pf6N2E2_660 0.36 -0.4
764,632 + Pf6N2E2_660 0.36 -0.2
764,633 - Pf6N2E2_660 0.37 -1.3
764,725 + Pf6N2E2_660 0.72 +0.6
764,726 - Pf6N2E2_660 0.72 -1.5
764,938 + +0.7
765,682 + Pf6N2E2_661 0.59 -0.2
765,682 + Pf6N2E2_661 0.59 -0.3
765,682 + Pf6N2E2_661 0.59 +0.6
765,683 - Pf6N2E2_661 0.59 +0.5
765,683 - Pf6N2E2_661 0.59 -0.2
765,683 - Pf6N2E2_661 0.59 +1.1
765,683 - Pf6N2E2_661 0.59 +0.6
765,835 + Pf6N2E2_661 0.72 +0.4
765,835 + Pf6N2E2_661 0.72 +2.0
765,836 - Pf6N2E2_661 0.72 -1.0
765,868 + Pf6N2E2_661 0.74 -1.1
765,869 - Pf6N2E2_661 0.74 +0.6
765,937 + Pf6N2E2_661 0.80 -0.3
765,977 + Pf6N2E2_661 0.83 +0.8
765,998 - Pf6N2E2_661 0.85 +1.4
766,051 + Pf6N2E2_661 0.89 +0.9
766,066 + +0.0
766,067 - +0.8
766,321 + +0.7
766,570 - Pf6N2E2_662 0.47 -0.7
766,653 + Pf6N2E2_662 0.73 -2.4
766,653 + Pf6N2E2_662 0.73 -2.2
766,654 - Pf6N2E2_662 0.73 -0.3
766,654 - Pf6N2E2_662 0.73 -1.5
766,654 - Pf6N2E2_662 0.73 +0.3
766,698 + Pf6N2E2_662 0.87 +0.6
766,698 + Pf6N2E2_662 0.87 +0.2
766,698 + Pf6N2E2_662 0.87 +1.2
766,699 - Pf6N2E2_662 0.87 +0.6
766,701 + Pf6N2E2_662 0.88 +0.6
766,701 + Pf6N2E2_662 0.88 -1.2
766,702 - Pf6N2E2_662 0.88 +0.0
766,702 - Pf6N2E2_662 0.88 -0.2
766,738 - +0.8

Or see this region's nucleotide sequence