Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5387 and Pf6N2E2_5388 are separated by 100 nucleotides Pf6N2E2_5388 and Pf6N2E2_5389 are separated by 810 nucleotides
Pf6N2E2_5387 - Alginate biosynthesis protein Alg8, at 6,191,646 to 6,193,127
_5387
Pf6N2E2_5388 - GDP-mannose 6-dehydrogenase (EC 1.1.1.132), at 6,193,228 to 6,194,544
_5388
Pf6N2E2_5389 - UPF0246 protein YaaA, at 6,195,355 to 6,196,134
_5389
Position (kb)
6193
6194
6195 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 6192.454 kb on + strand, within Pf6N2E2_5387 at 6192.455 kb on - strand, within Pf6N2E2_5387 at 6192.580 kb on - strand, within Pf6N2E2_5387 at 6192.629 kb on - strand, within Pf6N2E2_5387 at 6192.808 kb on + strand, within Pf6N2E2_5387 at 6192.809 kb on - strand, within Pf6N2E2_5387 at 6192.859 kb on + strand, within Pf6N2E2_5387 at 6192.859 kb on + strand, within Pf6N2E2_5387 at 6192.859 kb on + strand, within Pf6N2E2_5387 at 6192.860 kb on - strand, within Pf6N2E2_5387 at 6192.860 kb on - strand, within Pf6N2E2_5387 at 6192.860 kb on - strand, within Pf6N2E2_5387 at 6192.860 kb on - strand, within Pf6N2E2_5387 at 6192.898 kb on - strand, within Pf6N2E2_5387 at 6192.928 kb on + strand, within Pf6N2E2_5387 at 6192.928 kb on + strand, within Pf6N2E2_5387 at 6192.928 kb on + strand, within Pf6N2E2_5387 at 6192.928 kb on + strand, within Pf6N2E2_5387 at 6192.928 kb on + strand, within Pf6N2E2_5387 at 6192.929 kb on - strand, within Pf6N2E2_5387 at 6192.929 kb on - strand, within Pf6N2E2_5387 at 6192.974 kb on - strand, within Pf6N2E2_5387 at 6193.068 kb on + strand at 6193.069 kb on - strand at 6193.069 kb on - strand at 6193.069 kb on - strand at 6193.069 kb on - strand at 6193.078 kb on + strand at 6193.079 kb on - strand at 6193.127 kb on + strand at 6193.127 kb on + strand at 6193.127 kb on + strand at 6193.128 kb on - strand at 6193.128 kb on - strand at 6193.144 kb on + strand at 6193.145 kb on - strand at 6193.229 kb on + strand at 6193.229 kb on + strand at 6193.230 kb on - strand at 6193.230 kb on - strand at 6193.316 kb on - strand at 6193.442 kb on + strand, within Pf6N2E2_5388 at 6193.442 kb on + strand, within Pf6N2E2_5388 at 6193.442 kb on + strand, within Pf6N2E2_5388 at 6193.442 kb on + strand, within Pf6N2E2_5388 at 6193.443 kb on - strand, within Pf6N2E2_5388 at 6193.443 kb on - strand, within Pf6N2E2_5388 at 6193.443 kb on - strand, within Pf6N2E2_5388 at 6193.472 kb on + strand, within Pf6N2E2_5388 at 6193.473 kb on - strand, within Pf6N2E2_5388 at 6193.473 kb on - strand, within Pf6N2E2_5388 at 6193.473 kb on - strand, within Pf6N2E2_5388 at 6193.490 kb on + strand, within Pf6N2E2_5388 at 6193.490 kb on + strand, within Pf6N2E2_5388 at 6193.490 kb on + strand, within Pf6N2E2_5388 at 6193.491 kb on - strand, within Pf6N2E2_5388 at 6193.537 kb on + strand, within Pf6N2E2_5388 at 6193.537 kb on + strand, within Pf6N2E2_5388 at 6193.537 kb on + strand, within Pf6N2E2_5388 at 6193.538 kb on - strand, within Pf6N2E2_5388 at 6193.538 kb on - strand, within Pf6N2E2_5388 at 6193.712 kb on + strand, within Pf6N2E2_5388 at 6193.713 kb on - strand, within Pf6N2E2_5388 at 6193.775 kb on + strand, within Pf6N2E2_5388 at 6193.775 kb on + strand, within Pf6N2E2_5388 at 6193.775 kb on + strand, within Pf6N2E2_5388 at 6193.775 kb on + strand, within Pf6N2E2_5388 at 6193.775 kb on + strand, within Pf6N2E2_5388 at 6193.775 kb on + strand, within Pf6N2E2_5388 at 6193.775 kb on + strand, within Pf6N2E2_5388 at 6193.776 kb on - strand, within Pf6N2E2_5388 at 6193.776 kb on - strand, within Pf6N2E2_5388 at 6193.776 kb on - strand, within Pf6N2E2_5388 at 6193.776 kb on - strand, within Pf6N2E2_5388 at 6193.776 kb on - strand, within Pf6N2E2_5388 at 6193.776 kb on - strand, within Pf6N2E2_5388 at 6193.776 kb on - strand, within Pf6N2E2_5388 at 6193.778 kb on + strand, within Pf6N2E2_5388 at 6193.778 kb on + strand, within Pf6N2E2_5388 at 6193.778 kb on + strand, within Pf6N2E2_5388 at 6193.778 kb on + strand, within Pf6N2E2_5388 at 6193.779 kb on - strand, within Pf6N2E2_5388 at 6193.913 kb on + strand, within Pf6N2E2_5388 at 6193.914 kb on - strand, within Pf6N2E2_5388 at 6193.914 kb on - strand, within Pf6N2E2_5388 at 6193.914 kb on - strand, within Pf6N2E2_5388 at 6193.914 kb on - strand, within Pf6N2E2_5388 at 6193.973 kb on + strand, within Pf6N2E2_5388 at 6194.170 kb on + strand, within Pf6N2E2_5388 at 6194.170 kb on + strand, within Pf6N2E2_5388 at 6194.171 kb on - strand, within Pf6N2E2_5388 at 6194.185 kb on + strand, within Pf6N2E2_5388 at 6194.185 kb on + strand, within Pf6N2E2_5388 at 6194.185 kb on + strand, within Pf6N2E2_5388 at 6194.186 kb on - strand, within Pf6N2E2_5388 at 6194.186 kb on - strand, within Pf6N2E2_5388 at 6194.186 kb on - strand, within Pf6N2E2_5388 at 6194.186 kb on - strand, within Pf6N2E2_5388 at 6194.186 kb on - strand, within Pf6N2E2_5388 at 6194.186 kb on - strand, within Pf6N2E2_5388 at 6194.190 kb on + strand, within Pf6N2E2_5388 at 6194.252 kb on + strand, within Pf6N2E2_5388 at 6194.252 kb on + strand, within Pf6N2E2_5388 at 6194.253 kb on - strand, within Pf6N2E2_5388 at 6194.290 kb on - strand, within Pf6N2E2_5388 at 6194.459 kb on - strand at 6194.467 kb on + strand at 6194.467 kb on + strand at 6194.503 kb on + strand at 6194.504 kb on - strand at 6194.504 kb on - strand at 6194.530 kb on + strand at 6194.531 kb on - strand at 6194.536 kb on - strand at 6194.659 kb on + strand at 6194.659 kb on + strand at 6194.660 kb on - strand at 6194.731 kb on + strand at 6194.841 kb on + strand at 6194.842 kb on - strand at 6194.978 kb on + strand at 6195.015 kb on + strand at 6195.078 kb on + strand at 6195.155 kb on + strand at 6195.294 kb on + strand at 6195.294 kb on + strand at 6195.294 kb on + strand at 6195.294 kb on + strand at 6195.295 kb on - strand at 6195.306 kb on + strand at 6195.306 kb on + strand at 6195.343 kb on + strand at 6195.343 kb on + strand at 6195.344 kb on - strand at 6195.357 kb on - strand at 6195.430 kb on + strand at 6195.430 kb on + strand at 6195.430 kb on + strand at 6195.431 kb on - strand at 6195.431 kb on - strand at 6195.431 kb on - strand at 6195.431 kb on - strand at 6195.515 kb on + strand, within Pf6N2E2_5389 at 6195.515 kb on + strand, within Pf6N2E2_5389 at 6195.515 kb on + strand, within Pf6N2E2_5389 at 6195.533 kb on + strand, within Pf6N2E2_5389
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 6,192,454 + Pf6N2E2_5387 0.55 +1.2 6,192,455 - Pf6N2E2_5387 0.55 -0.8 6,192,580 - Pf6N2E2_5387 0.63 -0.1 6,192,629 - Pf6N2E2_5387 0.66 -1.0 6,192,808 + Pf6N2E2_5387 0.78 -2.6 6,192,809 - Pf6N2E2_5387 0.78 +0.8 6,192,859 + Pf6N2E2_5387 0.82 +0.8 6,192,859 + Pf6N2E2_5387 0.82 +1.2 6,192,859 + Pf6N2E2_5387 0.82 +2.2 6,192,860 - Pf6N2E2_5387 0.82 -0.8 6,192,860 - Pf6N2E2_5387 0.82 -0.9 6,192,860 - Pf6N2E2_5387 0.82 -0.5 6,192,860 - Pf6N2E2_5387 0.82 -0.9 6,192,898 - Pf6N2E2_5387 0.84 -0.2 6,192,928 + Pf6N2E2_5387 0.87 -0.4 6,192,928 + Pf6N2E2_5387 0.87 -0.5 6,192,928 + Pf6N2E2_5387 0.87 -0.4 6,192,928 + Pf6N2E2_5387 0.87 +0.4 6,192,928 + Pf6N2E2_5387 0.87 +0.2 6,192,929 - Pf6N2E2_5387 0.87 +0.3 6,192,929 - Pf6N2E2_5387 0.87 +0.1 6,192,974 - Pf6N2E2_5387 0.90 +0.5 6,193,068 + +0.2 6,193,069 - +2.0 6,193,069 - +0.5 6,193,069 - +1.1 6,193,069 - +3.2 6,193,078 + -0.1 6,193,079 - +0.6 6,193,127 + +0.2 6,193,127 + +0.3 6,193,127 + +0.4 6,193,128 - -0.9 6,193,128 - -2.5 6,193,144 + +0.2 6,193,145 - -0.3 6,193,229 + -0.1 6,193,229 + +0.2 6,193,230 - -0.2 6,193,230 - -0.8 6,193,316 - -0.9 6,193,442 + Pf6N2E2_5388 0.16 +0.0 6,193,442 + Pf6N2E2_5388 0.16 -0.1 6,193,442 + Pf6N2E2_5388 0.16 +1.4 6,193,442 + Pf6N2E2_5388 0.16 +0.2 6,193,443 - Pf6N2E2_5388 0.16 -0.5 6,193,443 - Pf6N2E2_5388 0.16 +1.5 6,193,443 - Pf6N2E2_5388 0.16 -0.1 6,193,472 + Pf6N2E2_5388 0.19 +1.4 6,193,473 - Pf6N2E2_5388 0.19 +0.3 6,193,473 - Pf6N2E2_5388 0.19 +1.2 6,193,473 - Pf6N2E2_5388 0.19 -0.8 6,193,490 + Pf6N2E2_5388 0.20 +0.1 6,193,490 + Pf6N2E2_5388 0.20 -1.0 6,193,490 + Pf6N2E2_5388 0.20 +0.5 6,193,491 - Pf6N2E2_5388 0.20 +0.4 6,193,537 + Pf6N2E2_5388 0.23 -0.1 6,193,537 + Pf6N2E2_5388 0.23 +1.2 6,193,537 + Pf6N2E2_5388 0.23 -0.1 6,193,538 - Pf6N2E2_5388 0.24 +0.1 6,193,538 - Pf6N2E2_5388 0.24 -0.1 6,193,712 + Pf6N2E2_5388 0.37 +0.2 6,193,713 - Pf6N2E2_5388 0.37 +0.3 6,193,775 + Pf6N2E2_5388 0.42 -0.7 6,193,775 + Pf6N2E2_5388 0.42 +2.0 6,193,775 + Pf6N2E2_5388 0.42 +0.5 6,193,775 + Pf6N2E2_5388 0.42 -0.5 6,193,775 + Pf6N2E2_5388 0.42 +0.2 6,193,775 + Pf6N2E2_5388 0.42 +0.0 6,193,775 + Pf6N2E2_5388 0.42 +0.4 6,193,776 - Pf6N2E2_5388 0.42 +0.1 6,193,776 - Pf6N2E2_5388 0.42 -0.2 6,193,776 - Pf6N2E2_5388 0.42 +0.1 6,193,776 - Pf6N2E2_5388 0.42 +0.3 6,193,776 - Pf6N2E2_5388 0.42 +0.4 6,193,776 - Pf6N2E2_5388 0.42 +0.0 6,193,776 - Pf6N2E2_5388 0.42 +0.4 6,193,778 + Pf6N2E2_5388 0.42 -0.1 6,193,778 + Pf6N2E2_5388 0.42 +0.7 6,193,778 + Pf6N2E2_5388 0.42 +0.7 6,193,778 + Pf6N2E2_5388 0.42 -0.1 6,193,779 - Pf6N2E2_5388 0.42 +0.7 6,193,913 + Pf6N2E2_5388 0.52 -0.4 6,193,914 - Pf6N2E2_5388 0.52 -0.4 6,193,914 - Pf6N2E2_5388 0.52 +0.2 6,193,914 - Pf6N2E2_5388 0.52 +0.4 6,193,914 - Pf6N2E2_5388 0.52 -0.0 6,193,973 + Pf6N2E2_5388 0.57 -0.2 6,194,170 + Pf6N2E2_5388 0.72 +0.8 6,194,170 + Pf6N2E2_5388 0.72 +0.3 6,194,171 - Pf6N2E2_5388 0.72 +0.6 6,194,185 + Pf6N2E2_5388 0.73 +0.4 6,194,185 + Pf6N2E2_5388 0.73 +0.7 6,194,185 + Pf6N2E2_5388 0.73 +0.2 6,194,186 - Pf6N2E2_5388 0.73 +0.9 6,194,186 - Pf6N2E2_5388 0.73 +0.0 6,194,186 - Pf6N2E2_5388 0.73 +0.8 6,194,186 - Pf6N2E2_5388 0.73 -0.1 6,194,186 - Pf6N2E2_5388 0.73 +0.6 6,194,186 - Pf6N2E2_5388 0.73 +1.8 6,194,190 + Pf6N2E2_5388 0.73 +0.9 6,194,252 + Pf6N2E2_5388 0.78 +1.1 6,194,252 + Pf6N2E2_5388 0.78 +2.0 6,194,253 - Pf6N2E2_5388 0.78 +0.4 6,194,290 - Pf6N2E2_5388 0.81 +1.4 6,194,459 - +0.8 6,194,467 + +1.2 6,194,467 + -0.2 6,194,503 + +1.9 6,194,504 - +0.1 6,194,504 - +0.5 6,194,530 + +1.1 6,194,531 - +0.6 6,194,536 - -0.1 6,194,659 + +0.6 6,194,659 + +0.6 6,194,660 - -0.9 6,194,731 + -0.1 6,194,841 + -0.9 6,194,842 - -1.8 6,194,978 + +0.1 6,195,015 + +0.7 6,195,078 + +1.1 6,195,155 + +0.2 6,195,294 + +1.6 6,195,294 + +0.8 6,195,294 + +0.7 6,195,294 + -1.2 6,195,295 - +0.3 6,195,306 + +0.1 6,195,306 + +1.1 6,195,343 + +0.9 6,195,343 + -0.5 6,195,344 - -1.5 6,195,357 - -2.0 6,195,430 + -0.3 6,195,430 + +0.9 6,195,430 + -0.2 6,195,431 - +0.9 6,195,431 - +0.0 6,195,431 - +1.2 6,195,431 - +0.4 6,195,515 + Pf6N2E2_5389 0.21 +0.8 6,195,515 + Pf6N2E2_5389 0.21 -0.2 6,195,515 + Pf6N2E2_5389 0.21 -0.4 6,195,533 + Pf6N2E2_5389 0.23 +0.2
Or see this region's nucleotide sequence