Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5159 and Pf6N2E2_5160 are separated by 154 nucleotides Pf6N2E2_5160 and Pf6N2E2_5161 are separated by 432 nucleotides
Pf6N2E2_5159 - glutamyl-Q-tRNA synthetase, at 5,948,455 to 5,949,336
_5159
Pf6N2E2_5160 - C4-type zinc finger protein, DksA/TraR family, at 5,949,491 to 5,949,853
_5160
Pf6N2E2_5161 - Valine--pyruvate aminotransferase (EC 2.6.1.66) ## AvtA, at 5,950,286 to 5,951,458
_5161
Position (kb)
5949
5950 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5948.652 kb on + strand, within Pf6N2E2_5159 at 5948.653 kb on - strand, within Pf6N2E2_5159 at 5948.735 kb on + strand, within Pf6N2E2_5159 at 5948.736 kb on - strand, within Pf6N2E2_5159 at 5948.736 kb on - strand, within Pf6N2E2_5159 at 5948.736 kb on - strand, within Pf6N2E2_5159 at 5948.829 kb on - strand, within Pf6N2E2_5159 at 5948.834 kb on + strand, within Pf6N2E2_5159 at 5948.835 kb on - strand, within Pf6N2E2_5159 at 5948.924 kb on + strand, within Pf6N2E2_5159 at 5948.924 kb on + strand, within Pf6N2E2_5159 at 5948.924 kb on + strand, within Pf6N2E2_5159 at 5948.924 kb on + strand, within Pf6N2E2_5159 at 5948.999 kb on + strand, within Pf6N2E2_5159 at 5948.999 kb on + strand, within Pf6N2E2_5159 at 5948.999 kb on + strand, within Pf6N2E2_5159 at 5949.000 kb on - strand, within Pf6N2E2_5159 at 5949.000 kb on - strand, within Pf6N2E2_5159 at 5949.012 kb on - strand, within Pf6N2E2_5159 at 5949.047 kb on + strand, within Pf6N2E2_5159 at 5949.047 kb on + strand, within Pf6N2E2_5159 at 5949.048 kb on - strand, within Pf6N2E2_5159 at 5949.089 kb on + strand, within Pf6N2E2_5159 at 5949.090 kb on - strand, within Pf6N2E2_5159 at 5949.090 kb on - strand, within Pf6N2E2_5159 at 5949.090 kb on - strand, within Pf6N2E2_5159 at 5949.090 kb on - strand, within Pf6N2E2_5159 at 5949.090 kb on - strand, within Pf6N2E2_5159 at 5949.143 kb on + strand, within Pf6N2E2_5159 at 5949.143 kb on + strand, within Pf6N2E2_5159 at 5949.144 kb on - strand, within Pf6N2E2_5159 at 5949.326 kb on + strand at 5949.327 kb on - strand at 5949.327 kb on - strand at 5949.392 kb on - strand at 5949.392 kb on - strand at 5949.457 kb on + strand at 5949.458 kb on - strand at 5949.492 kb on + strand at 5949.493 kb on - strand at 5949.616 kb on - strand, within Pf6N2E2_5160 at 5949.616 kb on - strand, within Pf6N2E2_5160 at 5949.855 kb on + strand at 5949.855 kb on + strand at 5949.855 kb on + strand at 5949.855 kb on + strand at 5949.856 kb on - strand at 5949.856 kb on - strand at 5949.856 kb on - strand at 5949.856 kb on - strand at 5949.856 kb on - strand at 5949.856 kb on - strand at 5949.879 kb on + strand at 5949.879 kb on + strand at 5949.879 kb on + strand at 5949.880 kb on - strand at 5949.880 kb on - strand at 5949.880 kb on - strand at 5949.880 kb on - strand at 5949.949 kb on + strand at 5949.949 kb on + strand at 5949.949 kb on + strand at 5949.957 kb on + strand at 5949.957 kb on + strand at 5949.957 kb on + strand at 5949.958 kb on - strand at 5949.958 kb on - strand at 5950.024 kb on + strand at 5950.024 kb on + strand at 5950.024 kb on + strand at 5950.025 kb on - strand at 5950.048 kb on - strand at 5950.084 kb on + strand at 5950.084 kb on + strand at 5950.084 kb on + strand at 5950.085 kb on - strand at 5950.117 kb on + strand at 5950.117 kb on + strand at 5950.140 kb on + strand at 5950.140 kb on + strand at 5950.141 kb on - strand at 5950.243 kb on + strand at 5950.243 kb on + strand at 5950.243 kb on + strand at 5950.244 kb on - strand at 5950.244 kb on - strand at 5950.252 kb on - strand at 5950.298 kb on + strand at 5950.298 kb on + strand at 5950.298 kb on + strand at 5950.298 kb on + strand at 5950.299 kb on - strand at 5950.478 kb on + strand, within Pf6N2E2_5161 at 5950.529 kb on + strand, within Pf6N2E2_5161 at 5950.529 kb on + strand, within Pf6N2E2_5161
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 5,948,652 + Pf6N2E2_5159 0.22 -2.2 5,948,653 - Pf6N2E2_5159 0.22 -0.0 5,948,735 + Pf6N2E2_5159 0.32 +1.6 5,948,736 - Pf6N2E2_5159 0.32 +0.6 5,948,736 - Pf6N2E2_5159 0.32 +0.0 5,948,736 - Pf6N2E2_5159 0.32 -0.2 5,948,829 - Pf6N2E2_5159 0.42 +0.6 5,948,834 + Pf6N2E2_5159 0.43 -2.9 5,948,835 - Pf6N2E2_5159 0.43 +0.7 5,948,924 + Pf6N2E2_5159 0.53 -1.0 5,948,924 + Pf6N2E2_5159 0.53 +0.8 5,948,924 + Pf6N2E2_5159 0.53 +0.1 5,948,924 + Pf6N2E2_5159 0.53 +0.1 5,948,999 + Pf6N2E2_5159 0.62 +1.7 5,948,999 + Pf6N2E2_5159 0.62 -1.0 5,948,999 + Pf6N2E2_5159 0.62 +0.0 5,949,000 - Pf6N2E2_5159 0.62 -0.1 5,949,000 - Pf6N2E2_5159 0.62 +0.1 5,949,012 - Pf6N2E2_5159 0.63 +0.5 5,949,047 + Pf6N2E2_5159 0.67 +0.3 5,949,047 + Pf6N2E2_5159 0.67 +1.3 5,949,048 - Pf6N2E2_5159 0.67 -0.3 5,949,089 + Pf6N2E2_5159 0.72 +0.9 5,949,090 - Pf6N2E2_5159 0.72 -1.4 5,949,090 - Pf6N2E2_5159 0.72 -0.2 5,949,090 - Pf6N2E2_5159 0.72 +0.4 5,949,090 - Pf6N2E2_5159 0.72 -0.9 5,949,090 - Pf6N2E2_5159 0.72 +0.3 5,949,143 + Pf6N2E2_5159 0.78 +0.7 5,949,143 + Pf6N2E2_5159 0.78 -1.4 5,949,144 - Pf6N2E2_5159 0.78 -0.7 5,949,326 + -0.1 5,949,327 - +0.6 5,949,327 - +1.1 5,949,392 - -0.3 5,949,392 - -0.1 5,949,457 + +0.0 5,949,458 - +0.9 5,949,492 + +0.8 5,949,493 - -0.1 5,949,616 - Pf6N2E2_5160 0.34 -2.7 5,949,616 - Pf6N2E2_5160 0.34 -2.0 5,949,855 + +0.2 5,949,855 + -1.5 5,949,855 + -2.1 5,949,855 + -1.5 5,949,856 - +0.0 5,949,856 - +1.2 5,949,856 - -1.5 5,949,856 - -1.1 5,949,856 - -2.6 5,949,856 - -0.4 5,949,879 + -1.1 5,949,879 + -0.5 5,949,879 + +0.3 5,949,880 - -1.4 5,949,880 - +0.3 5,949,880 - -1.5 5,949,880 - -1.5 5,949,949 + +0.1 5,949,949 + -1.0 5,949,949 + -0.1 5,949,957 + -1.4 5,949,957 + +0.3 5,949,957 + -1.1 5,949,958 - +0.1 5,949,958 - -0.6 5,950,024 + -1.0 5,950,024 + +0.0 5,950,024 + -0.5 5,950,025 - -1.7 5,950,048 - -0.6 5,950,084 + -0.0 5,950,084 + -0.1 5,950,084 + -0.5 5,950,085 - +1.5 5,950,117 + -0.1 5,950,117 + -0.6 5,950,140 + -0.2 5,950,140 + -0.2 5,950,141 - +0.0 5,950,243 + -1.0 5,950,243 + -0.5 5,950,243 + +0.4 5,950,244 - +1.2 5,950,244 - +0.6 5,950,252 - -0.1 5,950,298 + -0.4 5,950,298 + +0.0 5,950,298 + -0.4 5,950,298 + +0.7 5,950,299 - +0.8 5,950,478 + Pf6N2E2_5161 0.16 -0.5 5,950,529 + Pf6N2E2_5161 0.21 -1.0 5,950,529 + Pf6N2E2_5161 0.21 -0.8
Or see this region's nucleotide sequence