Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4639 and Pf6N2E2_4640 are separated by 176 nucleotides Pf6N2E2_4640 and Pf6N2E2_4641 are separated by 23 nucleotides Pf6N2E2_4641 and Pf6N2E2_4642 are separated by 87 nucleotides Pf6N2E2_4642 and Pf6N2E2_4643 are separated by 38 nucleotides
Pf6N2E2_4639 - Adenosylhomocysteinase (EC 3.3.1.1), at 5,368,863 to 5,370,272
_4639
Pf6N2E2_4640 - hypothetical protein, at 5,370,449 to 5,370,571
_4640
Pf6N2E2_4641 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 5,370,595 to 5,370,996
_4641
Pf6N2E2_4642 - Putative transport, at 5,371,084 to 5,371,965
_4642
Pf6N2E2_4643 - EXOU, at 5,372,004 to 5,373,731
_4643
Position (kb)
5371
5372 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 5370.290 kb on - strand at 5370.290 kb on - strand at 5370.290 kb on - strand at 5370.295 kb on - strand at 5370.295 kb on - strand at 5370.295 kb on - strand at 5370.295 kb on - strand at 5370.451 kb on - strand at 5370.453 kb on - strand at 5370.453 kb on - strand at 5370.517 kb on + strand, within Pf6N2E2_4640 at 5370.532 kb on - strand, within Pf6N2E2_4640 at 5370.700 kb on + strand, within Pf6N2E2_4641 at 5370.700 kb on + strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.701 kb on - strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.742 kb on + strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.743 kb on - strand, within Pf6N2E2_4641 at 5370.825 kb on + strand, within Pf6N2E2_4641 at 5370.958 kb on + strand at 5370.958 kb on + strand at 5370.959 kb on - strand at 5370.959 kb on - strand at 5370.959 kb on - strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.052 kb on + strand at 5371.053 kb on - strand at 5371.053 kb on - strand at 5371.053 kb on - strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.056 kb on + strand at 5371.057 kb on - strand at 5371.057 kb on - strand at 5371.057 kb on - strand at 5371.083 kb on + strand at 5371.083 kb on + strand at 5371.084 kb on - strand at 5371.200 kb on + strand, within Pf6N2E2_4642 at 5371.201 kb on - strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.522 kb on + strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642 at 5371.523 kb on - strand, within Pf6N2E2_4642 at 5371.713 kb on + strand, within Pf6N2E2_4642 at 5371.713 kb on + strand, within Pf6N2E2_4642 at 5371.714 kb on - strand, within Pf6N2E2_4642 at 5371.714 kb on - strand, within Pf6N2E2_4642 at 5371.714 kb on - strand, within Pf6N2E2_4642 at 5371.714 kb on - strand, within Pf6N2E2_4642 at 5371.714 kb on - strand, within Pf6N2E2_4642 at 5371.714 kb on - strand, within Pf6N2E2_4642 at 5371.714 kb on - strand, within Pf6N2E2_4642 at 5371.717 kb on + strand, within Pf6N2E2_4642 at 5371.717 kb on + strand, within Pf6N2E2_4642 at 5371.718 kb on - strand, within Pf6N2E2_4642 at 5371.718 kb on - strand, within Pf6N2E2_4642 at 5371.774 kb on + strand, within Pf6N2E2_4642 at 5371.774 kb on + strand, within Pf6N2E2_4642 at 5371.774 kb on + strand, within Pf6N2E2_4642 at 5371.866 kb on + strand, within Pf6N2E2_4642 at 5371.867 kb on - strand, within Pf6N2E2_4642 at 5371.923 kb on + strand at 5371.923 kb on + strand at 5371.923 kb on + strand at 5371.923 kb on + strand at 5371.923 kb on + strand at 5371.924 kb on - strand at 5371.924 kb on - strand at 5371.924 kb on - strand at 5371.981 kb on + strand at 5371.981 kb on + strand at 5371.981 kb on + strand at 5372.080 kb on + strand at 5372.081 kb on - strand at 5372.131 kb on - strand at 5372.131 kb on - strand at 5372.131 kb on - strand at 5372.131 kb on - strand at 5372.207 kb on + strand, within Pf6N2E2_4643 at 5372.244 kb on + strand, within Pf6N2E2_4643 at 5372.244 kb on + strand, within Pf6N2E2_4643 at 5372.244 kb on + strand, within Pf6N2E2_4643 at 5372.245 kb on - strand, within Pf6N2E2_4643 at 5372.245 kb on - strand, within Pf6N2E2_4643 at 5372.245 kb on - strand, within Pf6N2E2_4643 at 5372.535 kb on + strand, within Pf6N2E2_4643 at 5372.535 kb on + strand, within Pf6N2E2_4643 at 5372.535 kb on + strand, within Pf6N2E2_4643 at 5372.535 kb on + strand, within Pf6N2E2_4643 at 5372.536 kb on - strand, within Pf6N2E2_4643 at 5372.616 kb on + strand, within Pf6N2E2_4643 at 5372.616 kb on + strand, within Pf6N2E2_4643 at 5372.616 kb on + strand, within Pf6N2E2_4643 at 5372.616 kb on + strand, within Pf6N2E2_4643 at 5372.617 kb on - strand, within Pf6N2E2_4643 at 5372.617 kb on - strand, within Pf6N2E2_4643 at 5372.617 kb on - strand, within Pf6N2E2_4643 at 5372.617 kb on - strand, within Pf6N2E2_4643 at 5372.625 kb on + strand, within Pf6N2E2_4643 at 5372.677 kb on + strand, within Pf6N2E2_4643 at 5372.677 kb on + strand, within Pf6N2E2_4643 at 5372.678 kb on - strand, within Pf6N2E2_4643 at 5372.682 kb on + strand, within Pf6N2E2_4643 at 5372.768 kb on + strand, within Pf6N2E2_4643 at 5372.768 kb on + strand, within Pf6N2E2_4643 at 5372.768 kb on + strand, within Pf6N2E2_4643 at 5372.803 kb on + strand, within Pf6N2E2_4643 at 5372.803 kb on + strand, within Pf6N2E2_4643 at 5372.804 kb on - strand, within Pf6N2E2_4643 at 5372.804 kb on - strand, within Pf6N2E2_4643 at 5372.804 kb on - strand, within Pf6N2E2_4643 at 5372.804 kb on - strand, within Pf6N2E2_4643 at 5372.804 kb on - strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.840 kb on + strand, within Pf6N2E2_4643 at 5372.841 kb on - strand, within Pf6N2E2_4643 at 5372.841 kb on - strand, within Pf6N2E2_4643 at 5372.841 kb on - strand, within Pf6N2E2_4643 at 5372.841 kb on - strand, within Pf6N2E2_4643 at 5372.841 kb on - strand, within Pf6N2E2_4643 at 5372.841 kb on - strand, within Pf6N2E2_4643 at 5372.912 kb on + strand, within Pf6N2E2_4643
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 5,370,290 - +2.0 5,370,290 - +0.2 5,370,290 - +0.8 5,370,295 - -0.3 5,370,295 - -2.9 5,370,295 - +1.2 5,370,295 - +1.2 5,370,451 - -1.0 5,370,453 - +0.5 5,370,453 - +0.2 5,370,517 + Pf6N2E2_4640 0.55 -2.4 5,370,532 - Pf6N2E2_4640 0.67 -1.5 5,370,700 + Pf6N2E2_4641 0.26 +1.1 5,370,700 + Pf6N2E2_4641 0.26 -0.8 5,370,701 - Pf6N2E2_4641 0.26 -0.1 5,370,701 - Pf6N2E2_4641 0.26 -0.3 5,370,701 - Pf6N2E2_4641 0.26 +0.2 5,370,701 - Pf6N2E2_4641 0.26 -0.1 5,370,701 - Pf6N2E2_4641 0.26 -0.2 5,370,701 - Pf6N2E2_4641 0.26 -0.0 5,370,701 - Pf6N2E2_4641 0.26 -0.5 5,370,742 + Pf6N2E2_4641 0.37 -0.4 5,370,742 + Pf6N2E2_4641 0.37 -0.5 5,370,742 + Pf6N2E2_4641 0.37 -1.0 5,370,742 + Pf6N2E2_4641 0.37 +0.5 5,370,742 + Pf6N2E2_4641 0.37 +0.1 5,370,742 + Pf6N2E2_4641 0.37 -0.8 5,370,742 + Pf6N2E2_4641 0.37 -1.4 5,370,743 - Pf6N2E2_4641 0.37 +0.3 5,370,743 - Pf6N2E2_4641 0.37 -1.2 5,370,743 - Pf6N2E2_4641 0.37 -1.1 5,370,743 - Pf6N2E2_4641 0.37 -0.9 5,370,743 - Pf6N2E2_4641 0.37 -0.0 5,370,825 + Pf6N2E2_4641 0.57 -0.6 5,370,958 + +0.6 5,370,958 + -0.4 5,370,959 - -0.5 5,370,959 - +0.0 5,370,959 - +0.7 5,371,052 + -0.9 5,371,052 + +0.3 5,371,052 + -1.2 5,371,052 + +0.3 5,371,052 + +1.1 5,371,052 + -0.7 5,371,052 + +1.2 5,371,053 - +0.6 5,371,053 - -0.0 5,371,053 - +0.3 5,371,056 + +1.8 5,371,056 + -0.7 5,371,056 + +0.7 5,371,056 + +0.8 5,371,056 + -1.0 5,371,057 - -0.7 5,371,057 - +0.2 5,371,057 - +0.5 5,371,083 + +1.1 5,371,083 + -0.7 5,371,084 - -1.2 5,371,200 + Pf6N2E2_4642 0.13 -0.3 5,371,201 - Pf6N2E2_4642 0.13 +0.0 5,371,522 + Pf6N2E2_4642 0.50 +1.7 5,371,522 + Pf6N2E2_4642 0.50 +0.5 5,371,522 + Pf6N2E2_4642 0.50 +0.5 5,371,522 + Pf6N2E2_4642 0.50 +0.5 5,371,523 - Pf6N2E2_4642 0.50 -0.0 5,371,523 - Pf6N2E2_4642 0.50 +0.2 5,371,523 - Pf6N2E2_4642 0.50 -0.1 5,371,523 - Pf6N2E2_4642 0.50 +0.7 5,371,713 + Pf6N2E2_4642 0.71 +0.1 5,371,713 + Pf6N2E2_4642 0.71 +0.8 5,371,714 - Pf6N2E2_4642 0.71 +1.6 5,371,714 - Pf6N2E2_4642 0.71 -0.9 5,371,714 - Pf6N2E2_4642 0.71 +0.4 5,371,714 - Pf6N2E2_4642 0.71 -1.6 5,371,714 - Pf6N2E2_4642 0.71 -0.5 5,371,714 - Pf6N2E2_4642 0.71 +0.9 5,371,714 - Pf6N2E2_4642 0.71 +1.1 5,371,717 + Pf6N2E2_4642 0.72 +0.4 5,371,717 + Pf6N2E2_4642 0.72 +0.6 5,371,718 - Pf6N2E2_4642 0.72 +0.7 5,371,718 - Pf6N2E2_4642 0.72 -1.6 5,371,774 + Pf6N2E2_4642 0.78 -0.1 5,371,774 + Pf6N2E2_4642 0.78 +0.1 5,371,774 + Pf6N2E2_4642 0.78 +1.3 5,371,866 + Pf6N2E2_4642 0.89 -1.9 5,371,867 - Pf6N2E2_4642 0.89 +0.1 5,371,923 + -0.7 5,371,923 + +0.7 5,371,923 + +0.5 5,371,923 + +0.5 5,371,923 + -0.9 5,371,924 - -0.0 5,371,924 - +3.0 5,371,924 - -0.4 5,371,981 + -0.3 5,371,981 + +0.0 5,371,981 + -0.5 5,372,080 + -0.1 5,372,081 - +0.5 5,372,131 - -1.8 5,372,131 - +2.1 5,372,131 - -0.4 5,372,131 - -0.6 5,372,207 + Pf6N2E2_4643 0.12 +0.2 5,372,244 + Pf6N2E2_4643 0.14 -1.0 5,372,244 + Pf6N2E2_4643 0.14 +1.4 5,372,244 + Pf6N2E2_4643 0.14 +1.5 5,372,245 - Pf6N2E2_4643 0.14 -0.7 5,372,245 - Pf6N2E2_4643 0.14 -0.1 5,372,245 - Pf6N2E2_4643 0.14 -0.5 5,372,535 + Pf6N2E2_4643 0.31 -0.4 5,372,535 + Pf6N2E2_4643 0.31 +0.5 5,372,535 + Pf6N2E2_4643 0.31 +0.0 5,372,535 + Pf6N2E2_4643 0.31 +0.3 5,372,536 - Pf6N2E2_4643 0.31 +2.0 5,372,616 + Pf6N2E2_4643 0.35 -1.7 5,372,616 + Pf6N2E2_4643 0.35 -0.3 5,372,616 + Pf6N2E2_4643 0.35 -0.8 5,372,616 + Pf6N2E2_4643 0.35 -1.7 5,372,617 - Pf6N2E2_4643 0.35 -0.7 5,372,617 - Pf6N2E2_4643 0.35 +1.5 5,372,617 - Pf6N2E2_4643 0.35 +1.4 5,372,617 - Pf6N2E2_4643 0.35 -0.5 5,372,625 + Pf6N2E2_4643 0.36 +0.8 5,372,677 + Pf6N2E2_4643 0.39 +1.3 5,372,677 + Pf6N2E2_4643 0.39 -1.3 5,372,678 - Pf6N2E2_4643 0.39 +1.0 5,372,682 + Pf6N2E2_4643 0.39 -0.1 5,372,768 + Pf6N2E2_4643 0.44 +0.7 5,372,768 + Pf6N2E2_4643 0.44 -0.4 5,372,768 + Pf6N2E2_4643 0.44 -1.7 5,372,803 + Pf6N2E2_4643 0.46 -0.9 5,372,803 + Pf6N2E2_4643 0.46 -0.0 5,372,804 - Pf6N2E2_4643 0.46 -0.4 5,372,804 - Pf6N2E2_4643 0.46 +1.5 5,372,804 - Pf6N2E2_4643 0.46 +0.4 5,372,804 - Pf6N2E2_4643 0.46 -0.2 5,372,804 - Pf6N2E2_4643 0.46 +0.5 5,372,840 + Pf6N2E2_4643 0.48 -0.2 5,372,840 + Pf6N2E2_4643 0.48 +2.0 5,372,840 + Pf6N2E2_4643 0.48 -0.5 5,372,840 + Pf6N2E2_4643 0.48 -0.6 5,372,840 + Pf6N2E2_4643 0.48 -0.5 5,372,840 + Pf6N2E2_4643 0.48 -0.3 5,372,840 + Pf6N2E2_4643 0.48 +1.2 5,372,840 + Pf6N2E2_4643 0.48 -0.4 5,372,841 - Pf6N2E2_4643 0.48 -0.4 5,372,841 - Pf6N2E2_4643 0.48 +1.1 5,372,841 - Pf6N2E2_4643 0.48 -0.2 5,372,841 - Pf6N2E2_4643 0.48 -0.2 5,372,841 - Pf6N2E2_4643 0.48 +1.0 5,372,841 - Pf6N2E2_4643 0.48 +1.2 5,372,912 + Pf6N2E2_4643 0.53 +0.5
Or see this region's nucleotide sequence