Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_460 and Pf6N2E2_461 are separated by 290 nucleotides Pf6N2E2_461 and Pf6N2E2_462 are separated by 41 nucleotides
Pf6N2E2_460 - Glycerate kinase (EC 2.7.1.31), at 524,208 to 525,344
_460
Pf6N2E2_461 - Methyl-accepting chemotaxis protein, at 525,635 to 527,260
_461
Pf6N2E2_462 - Aspartate aminotransferase (EC 2.6.1.1), at 527,302 to 528,486
_462
Position (kb)
525
526
527
528 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 525.006 kb on - strand, within Pf6N2E2_460 at 525.207 kb on + strand, within Pf6N2E2_460 at 525.207 kb on + strand, within Pf6N2E2_460 at 525.207 kb on + strand, within Pf6N2E2_460 at 525.208 kb on - strand, within Pf6N2E2_460 at 525.361 kb on - strand at 525.361 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.566 kb on - strand at 525.576 kb on - strand at 525.718 kb on - strand at 525.938 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.977 kb on + strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 525.978 kb on - strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.007 kb on + strand, within Pf6N2E2_461 at 526.082 kb on - strand, within Pf6N2E2_461 at 526.188 kb on - strand, within Pf6N2E2_461 at 526.188 kb on - strand, within Pf6N2E2_461 at 526.213 kb on + strand, within Pf6N2E2_461 at 526.213 kb on + strand, within Pf6N2E2_461 at 526.213 kb on + strand, within Pf6N2E2_461 at 526.214 kb on - strand, within Pf6N2E2_461 at 527.263 kb on + strand at 527.264 kb on - strand at 527.264 kb on - strand at 527.264 kb on - strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.299 kb on + strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.300 kb on - strand at 527.494 kb on + strand, within Pf6N2E2_462 at 527.532 kb on + strand, within Pf6N2E2_462 at 527.533 kb on - strand, within Pf6N2E2_462 at 527.591 kb on + strand, within Pf6N2E2_462 at 527.617 kb on + strand, within Pf6N2E2_462 at 527.617 kb on + strand, within Pf6N2E2_462 at 527.617 kb on + strand, within Pf6N2E2_462 at 527.618 kb on - strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.677 kb on + strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.678 kb on - strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.686 kb on + strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.687 kb on - strand, within Pf6N2E2_462 at 527.921 kb on - strand, within Pf6N2E2_462 at 527.935 kb on + strand, within Pf6N2E2_462 at 527.936 kb on - strand, within Pf6N2E2_462 at 527.936 kb on - strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.096 kb on + strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.097 kb on - strand, within Pf6N2E2_462 at 528.188 kb on - strand, within Pf6N2E2_462
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 525,006 - Pf6N2E2_460 0.70 +0.2 525,207 + Pf6N2E2_460 0.88 +0.5 525,207 + Pf6N2E2_460 0.88 +0.2 525,207 + Pf6N2E2_460 0.88 -0.5 525,208 - Pf6N2E2_460 0.88 +0.1 525,361 - -0.7 525,361 - -0.3 525,566 - -0.0 525,566 - +0.6 525,566 - +0.1 525,566 - -0.4 525,566 - +0.8 525,566 - -0.3 525,566 - -2.5 525,566 - +1.4 525,566 - +0.5 525,576 - -1.4 525,718 - -1.1 525,938 + Pf6N2E2_461 0.19 +1.1 525,977 + Pf6N2E2_461 0.21 -0.1 525,977 + Pf6N2E2_461 0.21 +0.8 525,977 + Pf6N2E2_461 0.21 +0.5 525,977 + Pf6N2E2_461 0.21 +0.7 525,978 - Pf6N2E2_461 0.21 +0.6 525,978 - Pf6N2E2_461 0.21 +0.4 525,978 - Pf6N2E2_461 0.21 +1.1 525,978 - Pf6N2E2_461 0.21 -0.1 526,007 + Pf6N2E2_461 0.23 +1.0 526,007 + Pf6N2E2_461 0.23 +1.8 526,007 + Pf6N2E2_461 0.23 -1.1 526,007 + Pf6N2E2_461 0.23 -0.8 526,082 - Pf6N2E2_461 0.27 -1.7 526,188 - Pf6N2E2_461 0.34 -1.0 526,188 - Pf6N2E2_461 0.34 +0.2 526,213 + Pf6N2E2_461 0.36 +0.5 526,213 + Pf6N2E2_461 0.36 +0.6 526,213 + Pf6N2E2_461 0.36 +2.3 526,214 - Pf6N2E2_461 0.36 +0.7 527,263 + +0.1 527,264 - -0.1 527,264 - -0.5 527,264 - +3.1 527,299 + -1.0 527,299 + +1.8 527,299 + +0.4 527,299 + -0.4 527,299 + -1.5 527,299 + +1.1 527,299 + -0.1 527,299 + -2.0 527,299 + -0.1 527,299 + -1.4 527,300 - -1.0 527,300 - +0.4 527,300 - -0.6 527,300 - +0.5 527,300 - -1.2 527,300 - +0.5 527,300 - +2.4 527,494 + Pf6N2E2_462 0.16 +0.8 527,532 + Pf6N2E2_462 0.19 +0.8 527,533 - Pf6N2E2_462 0.19 -0.2 527,591 + Pf6N2E2_462 0.24 +1.1 527,617 + Pf6N2E2_462 0.27 -0.2 527,617 + Pf6N2E2_462 0.27 -1.3 527,617 + Pf6N2E2_462 0.27 -0.3 527,618 - Pf6N2E2_462 0.27 +0.6 527,677 + Pf6N2E2_462 0.32 +0.5 527,677 + Pf6N2E2_462 0.32 -1.9 527,677 + Pf6N2E2_462 0.32 +1.8 527,677 + Pf6N2E2_462 0.32 -2.4 527,677 + Pf6N2E2_462 0.32 +0.7 527,677 + Pf6N2E2_462 0.32 +1.8 527,677 + Pf6N2E2_462 0.32 +1.2 527,677 + Pf6N2E2_462 0.32 +1.3 527,678 - Pf6N2E2_462 0.32 -0.8 527,678 - Pf6N2E2_462 0.32 +0.8 527,678 - Pf6N2E2_462 0.32 -1.4 527,678 - Pf6N2E2_462 0.32 -0.7 527,678 - Pf6N2E2_462 0.32 +0.4 527,678 - Pf6N2E2_462 0.32 +1.0 527,678 - Pf6N2E2_462 0.32 -0.9 527,678 - Pf6N2E2_462 0.32 +1.8 527,678 - Pf6N2E2_462 0.32 -0.1 527,686 + Pf6N2E2_462 0.32 -0.2 527,686 + Pf6N2E2_462 0.32 -0.4 527,686 + Pf6N2E2_462 0.32 +0.8 527,686 + Pf6N2E2_462 0.32 -0.4 527,686 + Pf6N2E2_462 0.32 +0.6 527,686 + Pf6N2E2_462 0.32 +0.8 527,686 + Pf6N2E2_462 0.32 +0.2 527,687 - Pf6N2E2_462 0.32 -0.5 527,687 - Pf6N2E2_462 0.32 -0.5 527,687 - Pf6N2E2_462 0.32 +0.8 527,687 - Pf6N2E2_462 0.32 +0.3 527,687 - Pf6N2E2_462 0.32 -0.5 527,687 - Pf6N2E2_462 0.32 -0.5 527,687 - Pf6N2E2_462 0.32 -0.1 527,687 - Pf6N2E2_462 0.32 -0.5 527,921 - Pf6N2E2_462 0.52 -1.4 527,935 + Pf6N2E2_462 0.53 -2.5 527,936 - Pf6N2E2_462 0.54 -0.4 527,936 - Pf6N2E2_462 0.54 +0.0 528,096 + Pf6N2E2_462 0.67 +0.2 528,096 + Pf6N2E2_462 0.67 -0.4 528,096 + Pf6N2E2_462 0.67 -1.1 528,096 + Pf6N2E2_462 0.67 -0.4 528,096 + Pf6N2E2_462 0.67 -0.2 528,097 - Pf6N2E2_462 0.67 -1.8 528,097 - Pf6N2E2_462 0.67 +0.7 528,097 - Pf6N2E2_462 0.67 -0.8 528,097 - Pf6N2E2_462 0.67 -1.0 528,097 - Pf6N2E2_462 0.67 +0.7 528,097 - Pf6N2E2_462 0.67 -0.3 528,097 - Pf6N2E2_462 0.67 +0.5 528,188 - Pf6N2E2_462 0.75 -0.8
Or see this region's nucleotide sequence