Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2533 and Pf6N2E2_2534 are separated by 268 nucleotides Pf6N2E2_2534 and Pf6N2E2_2535 are separated by 12 nucleotides Pf6N2E2_2535 and Pf6N2E2_2536 are separated by 202 nucleotides
Pf6N2E2_2533 - Rhodanese domain protein UPF0176, at 2,992,076 to 2,993,017
_2533
Pf6N2E2_2534 - Cell division protein BolA, at 2,993,286 to 2,993,585
_2534
Pf6N2E2_2535 - FIG00954136: hypothetical protein, at 2,993,598 to 2,994,116
_2535
Pf6N2E2_2536 - Fumarate hydratase class II (EC 4.2.1.2), at 2,994,319 to 2,995,713
_2536
Position (kb)
2993
2994
2995 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2992.680 kb on + strand, within Pf6N2E2_2533 at 2992.680 kb on + strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.681 kb on - strand, within Pf6N2E2_2533 at 2992.700 kb on - strand, within Pf6N2E2_2533 at 2992.761 kb on + strand, within Pf6N2E2_2533 at 2992.762 kb on - strand, within Pf6N2E2_2533 at 2992.782 kb on + strand, within Pf6N2E2_2533 at 2992.962 kb on + strand at 2992.963 kb on - strand at 2992.984 kb on + strand at 2992.984 kb on + strand at 2992.984 kb on + strand at 2992.984 kb on + strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.985 kb on - strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.986 kb on + strand at 2992.987 kb on - strand at 2993.046 kb on - strand at 2993.046 kb on - strand at 2993.046 kb on - strand at 2993.103 kb on + strand at 2993.124 kb on - strand at 2993.124 kb on - strand at 2993.124 kb on - strand at 2993.124 kb on - strand at 2993.359 kb on + strand, within Pf6N2E2_2534 at 2993.360 kb on - strand, within Pf6N2E2_2534 at 2993.360 kb on - strand, within Pf6N2E2_2534 at 2993.413 kb on + strand, within Pf6N2E2_2534 at 2993.413 kb on + strand, within Pf6N2E2_2534 at 2993.414 kb on - strand, within Pf6N2E2_2534 at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.585 kb on + strand at 2993.586 kb on - strand at 2993.586 kb on - strand at 2993.770 kb on + strand, within Pf6N2E2_2535 at 2993.771 kb on - strand, within Pf6N2E2_2535 at 2993.771 kb on - strand, within Pf6N2E2_2535 at 2993.818 kb on + strand, within Pf6N2E2_2535 at 2993.819 kb on - strand, within Pf6N2E2_2535 at 2993.819 kb on - strand, within Pf6N2E2_2535 at 2993.894 kb on - strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.974 kb on + strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2993.975 kb on - strand, within Pf6N2E2_2535 at 2994.132 kb on + strand at 2994.133 kb on - strand at 2994.284 kb on - strand at 2994.284 kb on - strand at 2994.303 kb on + strand at 2994.871 kb on - strand, within Pf6N2E2_2536 at 2994.941 kb on - strand, within Pf6N2E2_2536
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 2,992,680 + Pf6N2E2_2533 0.64 +0.0 2,992,680 + Pf6N2E2_2533 0.64 -0.1 2,992,681 - Pf6N2E2_2533 0.64 -1.0 2,992,681 - Pf6N2E2_2533 0.64 -2.2 2,992,681 - Pf6N2E2_2533 0.64 +0.2 2,992,681 - Pf6N2E2_2533 0.64 -0.3 2,992,681 - Pf6N2E2_2533 0.64 -1.1 2,992,681 - Pf6N2E2_2533 0.64 -2.4 2,992,681 - Pf6N2E2_2533 0.64 -0.8 2,992,681 - Pf6N2E2_2533 0.64 +0.1 2,992,681 - Pf6N2E2_2533 0.64 -2.0 2,992,681 - Pf6N2E2_2533 0.64 -0.1 2,992,700 - Pf6N2E2_2533 0.66 -1.3 2,992,761 + Pf6N2E2_2533 0.73 +0.8 2,992,762 - Pf6N2E2_2533 0.73 -3.1 2,992,782 + Pf6N2E2_2533 0.75 +0.3 2,992,962 + -1.2 2,992,963 - +1.6 2,992,984 + -1.4 2,992,984 + -0.5 2,992,984 + -1.0 2,992,984 + +0.6 2,992,985 - -0.3 2,992,985 - -0.5 2,992,985 - -1.0 2,992,985 - +0.2 2,992,985 - -1.4 2,992,986 + +1.0 2,992,986 + +0.3 2,992,986 + -0.8 2,992,986 + +0.2 2,992,986 + -0.5 2,992,987 - -0.9 2,993,046 - -0.2 2,993,046 - -0.2 2,993,046 - +1.6 2,993,103 + +0.7 2,993,124 - -0.1 2,993,124 - +0.8 2,993,124 - +0.4 2,993,124 - -0.1 2,993,359 + Pf6N2E2_2534 0.24 +0.6 2,993,360 - Pf6N2E2_2534 0.25 +1.7 2,993,360 - Pf6N2E2_2534 0.25 +0.8 2,993,413 + Pf6N2E2_2534 0.42 -0.6 2,993,413 + Pf6N2E2_2534 0.42 +0.3 2,993,414 - Pf6N2E2_2534 0.43 +1.3 2,993,585 + -0.1 2,993,585 + +0.8 2,993,585 + +0.9 2,993,585 + +0.6 2,993,585 + +0.2 2,993,585 + +0.5 2,993,586 - +0.3 2,993,586 - -0.4 2,993,770 + Pf6N2E2_2535 0.33 +2.8 2,993,771 - Pf6N2E2_2535 0.33 +1.5 2,993,771 - Pf6N2E2_2535 0.33 +2.7 2,993,818 + Pf6N2E2_2535 0.42 +1.3 2,993,819 - Pf6N2E2_2535 0.43 +0.7 2,993,819 - Pf6N2E2_2535 0.43 +0.7 2,993,894 - Pf6N2E2_2535 0.57 +0.8 2,993,974 + Pf6N2E2_2535 0.72 -0.0 2,993,974 + Pf6N2E2_2535 0.72 +1.3 2,993,974 + Pf6N2E2_2535 0.72 +1.5 2,993,974 + Pf6N2E2_2535 0.72 +1.9 2,993,974 + Pf6N2E2_2535 0.72 +0.7 2,993,975 - Pf6N2E2_2535 0.73 +1.6 2,993,975 - Pf6N2E2_2535 0.73 +0.4 2,993,975 - Pf6N2E2_2535 0.73 +1.0 2,993,975 - Pf6N2E2_2535 0.73 +0.9 2,993,975 - Pf6N2E2_2535 0.73 +1.4 2,993,975 - Pf6N2E2_2535 0.73 +1.4 2,993,975 - Pf6N2E2_2535 0.73 +1.2 2,994,132 + -1.0 2,994,133 - +1.2 2,994,284 - +0.9 2,994,284 - -1.7 2,994,303 + +0.8 2,994,871 - Pf6N2E2_2536 0.40 +1.3 2,994,941 - Pf6N2E2_2536 0.45 -0.0
Or see this region's nucleotide sequence