Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1933 and Pf6N2E2_1934 are separated by 55 nucleotides Pf6N2E2_1934 and Pf6N2E2_1935 are separated by 63 nucleotides Pf6N2E2_1935 and Pf6N2E2_1936 are separated by 142 nucleotides
Pf6N2E2_1933 - TcuR: regulates tcuABC genes used in utilization of tricarballylate, at 2,289,767 to 2,290,699
_1933
Pf6N2E2_1934 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,290,755 to 2,291,549
_1934
Pf6N2E2_1935 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,291,613 to 2,292,257
_1935
Pf6N2E2_1936 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like, at 2,292,400 to 2,293,440
_1936
Position (kb)
2291
2292
2293 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2290.664 kb on + strand at 2290.713 kb on + strand at 2290.713 kb on + strand at 2290.757 kb on - strand at 2290.792 kb on + strand at 2290.792 kb on + strand at 2290.793 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.894 kb on + strand, within Pf6N2E2_1934 at 2290.895 kb on - strand, within Pf6N2E2_1934 at 2291.010 kb on + strand, within Pf6N2E2_1934 at 2291.011 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.083 kb on - strand, within Pf6N2E2_1934 at 2291.098 kb on + strand, within Pf6N2E2_1934 at 2291.099 kb on - strand, within Pf6N2E2_1934 at 2291.135 kb on + strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.137 kb on + strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.266 kb on - strand, within Pf6N2E2_1934 at 2291.304 kb on - strand, within Pf6N2E2_1934 at 2291.500 kb on - strand at 2291.509 kb on - strand at 2291.529 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.791 kb on - strand, within Pf6N2E2_1935 at 2291.889 kb on + strand, within Pf6N2E2_1935 at 2291.890 kb on - strand, within Pf6N2E2_1935 at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.281 kb on + strand at 2292.282 kb on - strand at 2292.282 kb on - strand at 2292.334 kb on - strand at 2292.401 kb on + strand at 2292.521 kb on + strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936 at 2292.595 kb on + strand, within Pf6N2E2_1936 at 2292.596 kb on - strand, within Pf6N2E2_1936 at 2292.596 kb on - strand, within Pf6N2E2_1936 at 2292.710 kb on + strand, within Pf6N2E2_1936 at 2292.710 kb on + strand, within Pf6N2E2_1936 at 2292.719 kb on - strand, within Pf6N2E2_1936 at 2292.814 kb on - strand, within Pf6N2E2_1936 at 2292.841 kb on + strand, within Pf6N2E2_1936 at 2292.901 kb on - strand, within Pf6N2E2_1936 at 2292.914 kb on + strand, within Pf6N2E2_1936 at 2292.981 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.008 kb on - strand, within Pf6N2E2_1936 at 2293.229 kb on - strand, within Pf6N2E2_1936
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 2,290,664 + -1.4 2,290,713 + -1.4 2,290,713 + -0.0 2,290,757 - -0.3 2,290,792 + -0.9 2,290,792 + +0.4 2,290,793 - +0.5 2,290,814 - -0.2 2,290,814 - +0.6 2,290,814 - +0.1 2,290,814 - +0.5 2,290,894 + Pf6N2E2_1934 0.17 +1.2 2,290,895 - Pf6N2E2_1934 0.18 -1.6 2,291,010 + Pf6N2E2_1934 0.32 -0.4 2,291,011 - Pf6N2E2_1934 0.32 -1.9 2,291,056 - Pf6N2E2_1934 0.38 -0.2 2,291,056 - Pf6N2E2_1934 0.38 -0.0 2,291,083 - Pf6N2E2_1934 0.41 +0.6 2,291,098 + Pf6N2E2_1934 0.43 +0.3 2,291,099 - Pf6N2E2_1934 0.43 +1.0 2,291,135 + Pf6N2E2_1934 0.48 +0.1 2,291,136 - Pf6N2E2_1934 0.48 +0.2 2,291,136 - Pf6N2E2_1934 0.48 +0.6 2,291,137 + Pf6N2E2_1934 0.48 +0.1 2,291,138 - Pf6N2E2_1934 0.48 -2.4 2,291,138 - Pf6N2E2_1934 0.48 -1.4 2,291,138 - Pf6N2E2_1934 0.48 -0.2 2,291,138 - Pf6N2E2_1934 0.48 -1.1 2,291,138 - Pf6N2E2_1934 0.48 -1.5 2,291,266 - Pf6N2E2_1934 0.64 -0.3 2,291,304 - Pf6N2E2_1934 0.69 -1.0 2,291,500 - +0.2 2,291,509 - -1.0 2,291,529 - -0.4 2,291,553 - -0.6 2,291,553 - +0.4 2,291,553 - +0.6 2,291,553 - -0.2 2,291,553 - +1.9 2,291,553 - -0.1 2,291,790 + Pf6N2E2_1935 0.27 +0.5 2,291,790 + Pf6N2E2_1935 0.27 -1.8 2,291,791 - Pf6N2E2_1935 0.28 +0.2 2,291,889 + Pf6N2E2_1935 0.43 -1.1 2,291,890 - Pf6N2E2_1935 0.43 -1.5 2,292,234 - +0.8 2,292,234 - -0.9 2,292,234 - -1.5 2,292,281 + -0.1 2,292,282 - -0.9 2,292,282 - -0.5 2,292,334 - -0.2 2,292,401 + -1.4 2,292,521 + Pf6N2E2_1936 0.12 +0.5 2,292,522 - Pf6N2E2_1936 0.12 +1.0 2,292,522 - Pf6N2E2_1936 0.12 +0.4 2,292,595 + Pf6N2E2_1936 0.19 -2.5 2,292,596 - Pf6N2E2_1936 0.19 -0.6 2,292,596 - Pf6N2E2_1936 0.19 -1.6 2,292,710 + Pf6N2E2_1936 0.30 +0.6 2,292,710 + Pf6N2E2_1936 0.30 +1.8 2,292,719 - Pf6N2E2_1936 0.31 -1.9 2,292,814 - Pf6N2E2_1936 0.40 -1.2 2,292,841 + Pf6N2E2_1936 0.42 +1.4 2,292,901 - Pf6N2E2_1936 0.48 -0.0 2,292,914 + Pf6N2E2_1936 0.49 +2.1 2,292,981 + Pf6N2E2_1936 0.56 -0.4 2,293,007 + Pf6N2E2_1936 0.58 -0.3 2,293,007 + Pf6N2E2_1936 0.58 -1.5 2,293,007 + Pf6N2E2_1936 0.58 -0.1 2,293,007 + Pf6N2E2_1936 0.58 +0.8 2,293,008 - Pf6N2E2_1936 0.58 -2.0 2,293,229 - Pf6N2E2_1936 0.80 +0.2
Or see this region's nucleotide sequence