Experiment: thiosulfate 0.25 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1547 and Pf6N2E2_1548 are separated by 103 nucleotides Pf6N2E2_1548 and Pf6N2E2_1549 are separated by 23 nucleotides
Pf6N2E2_1547 - Permease of the drug/metabolite transporter (DMT) superfamily, at 1,806,431 to 1,807,387
_1547
Pf6N2E2_1548 - Lactoylglutathione lyase, at 1,807,491 to 1,807,877
_1548
Pf6N2E2_1549 - Transcriptional regulator, LysR family, at 1,807,901 to 1,808,833
_1549
Position (kb)
1807
1808 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1806.570 kb on - strand, within Pf6N2E2_1547 at 1806.771 kb on + strand, within Pf6N2E2_1547 at 1806.772 kb on - strand, within Pf6N2E2_1547 at 1806.858 kb on + strand, within Pf6N2E2_1547 at 1806.858 kb on + strand, within Pf6N2E2_1547 at 1806.859 kb on - strand, within Pf6N2E2_1547 at 1806.989 kb on + strand, within Pf6N2E2_1547 at 1806.990 kb on - strand, within Pf6N2E2_1547 at 1806.990 kb on - strand, within Pf6N2E2_1547 at 1806.990 kb on - strand, within Pf6N2E2_1547 at 1807.223 kb on + strand, within Pf6N2E2_1547 at 1807.223 kb on + strand, within Pf6N2E2_1547 at 1807.224 kb on - strand, within Pf6N2E2_1547 at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.414 kb on - strand at 1807.427 kb on + strand at 1807.498 kb on + strand at 1807.499 kb on - strand at 1807.499 kb on - strand at 1807.732 kb on - strand, within Pf6N2E2_1548 at 1807.804 kb on - strand, within Pf6N2E2_1548 at 1807.804 kb on - strand, within Pf6N2E2_1548 at 1807.806 kb on + strand, within Pf6N2E2_1548 at 1807.815 kb on + strand, within Pf6N2E2_1548 at 1807.816 kb on - strand, within Pf6N2E2_1548 at 1807.875 kb on + strand at 1807.875 kb on + strand at 1807.961 kb on + strand at 1807.962 kb on - strand at 1808.097 kb on - strand, within Pf6N2E2_1549 at 1808.244 kb on + strand, within Pf6N2E2_1549 at 1808.307 kb on + strand, within Pf6N2E2_1549 at 1808.308 kb on - strand, within Pf6N2E2_1549 at 1808.431 kb on + strand, within Pf6N2E2_1549 at 1808.432 kb on - strand, within Pf6N2E2_1549 at 1808.497 kb on + strand, within Pf6N2E2_1549 at 1808.623 kb on + strand, within Pf6N2E2_1549 at 1808.672 kb on - strand, within Pf6N2E2_1549 at 1808.672 kb on - strand, within Pf6N2E2_1549 at 1808.672 kb on - strand, within Pf6N2E2_1549 at 1808.798 kb on + strand at 1808.798 kb on + strand at 1808.798 kb on + strand at 1808.799 kb on - strand at 1808.799 kb on - strand at 1808.799 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction thiosulfate 0.25 M remove 1,806,570 - Pf6N2E2_1547 0.15 +1.6 1,806,771 + Pf6N2E2_1547 0.36 -0.7 1,806,772 - Pf6N2E2_1547 0.36 -0.1 1,806,858 + Pf6N2E2_1547 0.45 +1.0 1,806,858 + Pf6N2E2_1547 0.45 -1.0 1,806,859 - Pf6N2E2_1547 0.45 +0.3 1,806,989 + Pf6N2E2_1547 0.58 -0.8 1,806,990 - Pf6N2E2_1547 0.58 +0.9 1,806,990 - Pf6N2E2_1547 0.58 -0.6 1,806,990 - Pf6N2E2_1547 0.58 -0.7 1,807,223 + Pf6N2E2_1547 0.83 +0.4 1,807,223 + Pf6N2E2_1547 0.83 -1.9 1,807,224 - Pf6N2E2_1547 0.83 -1.6 1,807,414 - +2.7 1,807,414 - -0.5 1,807,414 - +0.4 1,807,414 - -0.8 1,807,414 - +0.7 1,807,427 + -0.6 1,807,498 + +0.4 1,807,499 - +0.9 1,807,499 - +0.1 1,807,732 - Pf6N2E2_1548 0.62 -2.5 1,807,804 - Pf6N2E2_1548 0.81 -2.9 1,807,804 - Pf6N2E2_1548 0.81 -1.5 1,807,806 + Pf6N2E2_1548 0.81 +0.7 1,807,815 + Pf6N2E2_1548 0.84 -0.0 1,807,816 - Pf6N2E2_1548 0.84 -1.0 1,807,875 + -1.2 1,807,875 + -0.8 1,807,961 + -1.0 1,807,962 - -0.8 1,808,097 - Pf6N2E2_1549 0.21 +2.7 1,808,244 + Pf6N2E2_1549 0.37 +1.1 1,808,307 + Pf6N2E2_1549 0.44 -0.2 1,808,308 - Pf6N2E2_1549 0.44 -0.5 1,808,431 + Pf6N2E2_1549 0.57 +0.7 1,808,432 - Pf6N2E2_1549 0.57 -2.2 1,808,497 + Pf6N2E2_1549 0.64 -0.7 1,808,623 + Pf6N2E2_1549 0.77 +0.7 1,808,672 - Pf6N2E2_1549 0.83 -2.7 1,808,672 - Pf6N2E2_1549 0.83 +0.3 1,808,672 - Pf6N2E2_1549 0.83 +1.7 1,808,798 + +1.2 1,808,798 + +0.8 1,808,798 + -0.1 1,808,799 - +1.3 1,808,799 - +0.2 1,808,799 - -0.1
Or see this region's nucleotide sequence