Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00970 and MMP_RS00975 overlap by 4 nucleotides MMP_RS00975 and MMP_RS00980 are separated by 142 nucleotides MMP_RS00980 and MMP_RS00985 are separated by 24 nucleotides
MMP_RS00970: MMP_RS00970 - coenzyme F430 synthase, at 180,497 to 181,699
_RS00970
MMP_RS00975: MMP_RS00975 - HD domain-containing protein, at 181,696 to 183,072
_RS00975
MMP_RS00980: MMP_RS00980 - hypothetical protein, at 183,215 to 183,679
_RS00980
MMP_RS00985: MMP_RS00985 - CDC48 family AAA ATPase, at 183,704 to 186,070
_RS00985
Position (kb)
181
182
183
184 Strain fitness (log2 ratio)
-2
-1
0
1 at 180.924 kb on - strand at 180.933 kb on - strand at 181.086 kb on - strand at 181.086 kb on - strand at 181.149 kb on - strand at 181.151 kb on - strand at 181.152 kb on + strand at 181.291 kb on + strand at 181.406 kb on + strand at 181.434 kb on - strand at 181.464 kb on - strand at 181.482 kb on - strand at 181.501 kb on + strand at 181.621 kb on - strand at 181.636 kb on + strand at 181.666 kb on - strand at 181.731 kb on - strand at 181.883 kb on + strand, within MMP_RS00975 at 181.896 kb on - strand, within MMP_RS00975 at 182.019 kb on + strand, within MMP_RS00975 at 182.091 kb on - strand, within MMP_RS00975 at 182.092 kb on + strand, within MMP_RS00975 at 182.132 kb on + strand, within MMP_RS00975 at 182.198 kb on + strand, within MMP_RS00975 at 182.281 kb on - strand, within MMP_RS00975 at 182.293 kb on + strand, within MMP_RS00975 at 182.328 kb on - strand, within MMP_RS00975 at 182.328 kb on - strand, within MMP_RS00975 at 182.329 kb on + strand, within MMP_RS00975 at 182.332 kb on - strand, within MMP_RS00975 at 182.359 kb on - strand, within MMP_RS00975 at 182.399 kb on + strand, within MMP_RS00975 at 182.579 kb on + strand, within MMP_RS00975 at 182.621 kb on + strand, within MMP_RS00975 at 182.696 kb on + strand, within MMP_RS00975 at 182.714 kb on - strand, within MMP_RS00975 at 182.715 kb on + strand, within MMP_RS00975 at 182.782 kb on - strand, within MMP_RS00975 at 182.895 kb on - strand, within MMP_RS00975 at 182.950 kb on + strand at 183.001 kb on - strand at 183.007 kb on - strand at 183.022 kb on + strand at 183.031 kb on + strand at 183.082 kb on - strand at 183.096 kb on - strand at 183.114 kb on - strand at 183.114 kb on - strand at 183.160 kb on - strand at 183.160 kb on - strand at 183.161 kb on + strand at 183.164 kb on - strand at 183.164 kb on - strand at 183.165 kb on + strand at 183.165 kb on + strand at 183.172 kb on + strand at 183.192 kb on - strand at 183.198 kb on - strand at 183.198 kb on - strand at 183.198 kb on - strand at 183.236 kb on - strand at 183.248 kb on + strand at 183.248 kb on + strand at 183.344 kb on - strand, within MMP_RS00980 at 183.345 kb on + strand, within MMP_RS00980 at 183.345 kb on + strand, within MMP_RS00980 at 183.350 kb on - strand, within MMP_RS00980 at 183.351 kb on + strand, within MMP_RS00980 at 183.370 kb on - strand, within MMP_RS00980 at 183.371 kb on + strand, within MMP_RS00980 at 183.467 kb on - strand, within MMP_RS00980 at 183.540 kb on - strand, within MMP_RS00980 at 183.552 kb on - strand, within MMP_RS00980 at 183.611 kb on - strand, within MMP_RS00980 at 183.611 kb on - strand, within MMP_RS00980 at 183.612 kb on + strand, within MMP_RS00980 at 183.613 kb on - strand, within MMP_RS00980 at 183.613 kb on - strand, within MMP_RS00980 at 183.613 kb on - strand, within MMP_RS00980 at 183.614 kb on + strand, within MMP_RS00980 at 183.618 kb on - strand, within MMP_RS00980 at 183.619 kb on + strand, within MMP_RS00980 at 183.619 kb on + strand, within MMP_RS00980 at 183.634 kb on - strand at 183.689 kb on + strand at 183.689 kb on + strand at 183.800 kb on + strand at 183.813 kb on + strand at 183.923 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 180,924 - -1.3 180,933 - -2.0 181,086 - -2.6 181,086 - -1.1 181,149 - -0.8 181,151 - -1.0 181,152 + -1.2 181,291 + -0.5 181,406 + -1.6 181,434 - -1.3 181,464 - -1.3 181,482 - -1.1 181,501 + -2.1 181,621 - -0.9 181,636 + -0.7 181,666 - -2.7 181,731 - -0.2 181,883 + MMP_RS00975 0.14 +0.4 181,896 - MMP_RS00975 0.15 +0.2 182,019 + MMP_RS00975 0.23 +0.1 182,091 - MMP_RS00975 0.29 +0.2 182,092 + MMP_RS00975 0.29 +0.5 182,132 + MMP_RS00975 0.32 -0.2 182,198 + MMP_RS00975 0.36 -0.2 182,281 - MMP_RS00975 0.42 -0.1 182,293 + MMP_RS00975 0.43 +0.6 182,328 - MMP_RS00975 0.46 +0.2 182,328 - MMP_RS00975 0.46 -0.3 182,329 + MMP_RS00975 0.46 -0.1 182,332 - MMP_RS00975 0.46 +0.0 182,359 - MMP_RS00975 0.48 +0.1 182,399 + MMP_RS00975 0.51 +0.1 182,579 + MMP_RS00975 0.64 +0.1 182,621 + MMP_RS00975 0.67 +0.8 182,696 + MMP_RS00975 0.73 -0.2 182,714 - MMP_RS00975 0.74 -0.2 182,715 + MMP_RS00975 0.74 +0.1 182,782 - MMP_RS00975 0.79 -0.1 182,895 - MMP_RS00975 0.87 -0.1 182,950 + -0.2 183,001 - -0.7 183,007 - -0.1 183,022 + -0.0 183,031 + +0.3 183,082 - +1.1 183,096 - +0.5 183,114 - -0.2 183,114 - -0.0 183,160 - +0.5 183,160 - -0.4 183,161 + +0.2 183,164 - +0.1 183,164 - +0.1 183,165 + +0.3 183,165 + -0.0 183,172 + +0.1 183,192 - -0.7 183,198 - -0.5 183,198 - -0.0 183,198 - +0.1 183,236 - +0.8 183,248 + +0.1 183,248 + -0.2 183,344 - MMP_RS00980 0.28 -0.1 183,345 + MMP_RS00980 0.28 -0.6 183,345 + MMP_RS00980 0.28 +0.1 183,350 - MMP_RS00980 0.29 -0.1 183,351 + MMP_RS00980 0.29 +0.5 183,370 - MMP_RS00980 0.33 +0.6 183,371 + MMP_RS00980 0.34 -0.1 183,467 - MMP_RS00980 0.54 -0.1 183,540 - MMP_RS00980 0.70 -0.3 183,552 - MMP_RS00980 0.72 -0.3 183,611 - MMP_RS00980 0.85 +0.5 183,611 - MMP_RS00980 0.85 -0.1 183,612 + MMP_RS00980 0.85 -0.1 183,613 - MMP_RS00980 0.86 -0.2 183,613 - MMP_RS00980 0.86 -0.4 183,613 - MMP_RS00980 0.86 +0.1 183,614 + MMP_RS00980 0.86 +0.5 183,618 - MMP_RS00980 0.87 +0.1 183,619 + MMP_RS00980 0.87 -0.0 183,619 + MMP_RS00980 0.87 +0.0 183,634 - -0.2 183,689 + +0.2 183,689 + +0.3 183,800 + -0.1 183,813 + -0.2 183,923 - -0.0
Or see this region's nucleotide sequence