Experiment: With vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13410 and BPHYT_RS13415 are separated by 26 nucleotides BPHYT_RS13415 and BPHYT_RS13420 are separated by 124 nucleotides BPHYT_RS13420 and BPHYT_RS13425 overlap by 4 nucleotides BPHYT_RS13425 and BPHYT_RS13430 are separated by 2 nucleotides
BPHYT_RS13410: BPHYT_RS13410 - FAD-dependent oxidoreductase, at 3,063,017 to 3,064,306
_RS13410
BPHYT_RS13415: BPHYT_RS13415 - hypothetical protein, at 3,064,333 to 3,064,617
_RS13415
BPHYT_RS13420: BPHYT_RS13420 - hypothetical protein, at 3,064,742 to 3,065,725
_RS13420
BPHYT_RS13425: BPHYT_RS13425 - histone acetyltransferase, at 3,065,722 to 3,066,333
_RS13425
BPHYT_RS13430: BPHYT_RS13430 - radical SAM protein, at 3,066,336 to 3,067,406
_RS13430
Position (kb) 3064
3065
3066 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 3064.033 kb on - strand, within BPHYT_RS13410 at 3064.267 kb on - strand at 3064.515 kb on - strand, within BPHYT_RS13415 at 3064.886 kb on + strand, within BPHYT_RS13420 at 3065.059 kb on + strand, within BPHYT_RS13420 at 3065.451 kb on + strand, within BPHYT_RS13420 at 3065.455 kb on + strand, within BPHYT_RS13420 at 3066.151 kb on - strand, within BPHYT_RS13425 at 3066.347 kb on - strand at 3066.607 kb on + strand, within BPHYT_RS13430 at 3066.665 kb on - strand, within BPHYT_RS13430
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t1 remove 3,064,033 - BPHYT_RS13410 0.79 -0.2 3,064,267 - +0.1 3,064,515 - BPHYT_RS13415 0.64 +2.9 3,064,886 + BPHYT_RS13420 0.15 -1.1 3,065,059 + BPHYT_RS13420 0.32 -0.1 3,065,451 + BPHYT_RS13420 0.72 -1.0 3,065,455 + BPHYT_RS13420 0.72 +0.2 3,066,151 - BPHYT_RS13425 0.70 -1.9 3,066,347 - +0.9 3,066,607 + BPHYT_RS13430 0.25 +0.6 3,066,665 - BPHYT_RS13430 0.31 +0.7
Or see this region's nucleotide sequence