Experiment: Anaero. fumarate as E acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rplT and rpmI are separated by 18 nucleotides rpmI and Shew_2031 are separated by 87 nucleotides Shew_2031 and Shew_2032 are separated by 24 nucleotides Shew_2032 and Shew_2033 are separated by 315 nucleotides Shew_2033 and Shew_2034 are separated by 150 nucleotides
Shew_2029: rplT - 50S ribosomal protein L20 (RefSeq), at 2,343,311 to 2,343,670
rplT
Shew_2030: rpmI - 50S ribosomal protein L35 (RefSeq), at 2,343,689 to 2,343,883
rpmI
Shew_2031: Shew_2031 - translation initiation factor IF-3 (RefSeq), at 2,343,971 to 2,344,492
_2031
Shew_2032: Shew_2032 - threonyl-tRNA synthetase (RefSeq), at 2,344,517 to 2,346,445
_2032
Shew_2033: Shew_2033 - hypothetical protein (RefSeq), at 2,346,761 to 2,347,036
_2033
Shew_2034: Shew_2034 - short-chain dehydrogenase/reductase SDR (RefSeq), at 2,347,187 to 2,347,933
_2034
Position (kb)
2344
2345
2346
2347 Strain fitness (log2 ratio)
-1
0
1 at 2346.595 kb on + strand at 2346.604 kb on - strand at 2346.967 kb on + strand, within Shew_2033 at 2346.967 kb on + strand, within Shew_2033 at 2347.093 kb on + strand at 2347.208 kb on - strand at 2347.409 kb on - strand, within Shew_2034
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. fumarate as E acceptor remove 2,346,595 + +0.2 2,346,604 - +1.3 2,346,967 + Shew_2033 0.75 +0.0 2,346,967 + Shew_2033 0.75 +1.2 2,347,093 + -0.2 2,347,208 - -0.7 2,347,409 - Shew_2034 0.30 -1.1
Or see this region's nucleotide sequence