Experiment: Anaero. fumarate as E acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1860 and Shew_1861 are separated by 6 nucleotides Shew_1861 and Shew_1862 overlap by 7 nucleotides Shew_1862 and Shew_1863 overlap by 4 nucleotides Shew_1863 and Shew_1864 are separated by 286 nucleotides
Shew_1860: Shew_1860 - alpha/beta hydrolase fold (RefSeq), at 2,139,297 to 2,140,130
_1860
Shew_1861: Shew_1861 - peptidase M15B and M15C, D,D-carboxypeptidase VanY/endolysin (RefSeq), at 2,140,137 to 2,140,889
_1861
Shew_1862: Shew_1862 - succinyl-diaminopimelate desuccinylase (RefSeq), at 2,140,883 to 2,142,013
_1862
Shew_1863: Shew_1863 - arsenate reductase and related (RefSeq), at 2,142,010 to 2,142,354
_1863
Shew_1864: Shew_1864 - glucose-specific PTS system component (RefSeq), at 2,142,641 to 2,143,150
_1864
Position (kb)
2140
2141
2142
2143 Strain fitness (log2 ratio)
-2
-1
0
1 at 2139.886 kb on - strand, within Shew_1860 at 2139.902 kb on - strand, within Shew_1860 at 2140.028 kb on - strand, within Shew_1860 at 2140.275 kb on - strand, within Shew_1861 at 2140.276 kb on - strand, within Shew_1861 at 2140.620 kb on + strand, within Shew_1861 at 2140.628 kb on - strand, within Shew_1861 at 2140.628 kb on - strand, within Shew_1861 at 2140.628 kb on - strand, within Shew_1861 at 2142.647 kb on - strand at 2142.896 kb on + strand, within Shew_1864 at 2143.003 kb on - strand, within Shew_1864
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. fumarate as E acceptor remove 2,139,886 - Shew_1860 0.71 +0.0 2,139,902 - Shew_1860 0.73 +0.1 2,140,028 - Shew_1860 0.88 -0.1 2,140,275 - Shew_1861 0.18 +0.6 2,140,276 - Shew_1861 0.18 +0.8 2,140,620 + Shew_1861 0.64 -2.3 2,140,628 - Shew_1861 0.65 +1.2 2,140,628 - Shew_1861 0.65 +1.2 2,140,628 - Shew_1861 0.65 -1.5 2,142,647 - +1.0 2,142,896 + Shew_1864 0.50 +0.1 2,143,003 - Shew_1864 0.71 +0.9
Or see this region's nucleotide sequence