Experiment: Anaero. fumarate as E acceptor
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0644 and Shew_0645 are separated by 9 nucleotides Shew_0645 and Shew_0646 overlap by 4 nucleotides Shew_0646 and Shew_0647 are separated by 14 nucleotides
Shew_0644: Shew_0644 - FHA domain-containing protein (RefSeq), at 732,169 to 733,878
_0644
Shew_0645: Shew_0645 - type II and III secretion system protein (RefSeq), at 733,888 to 735,198
_0645
Shew_0646: Shew_0646 - SAF domain-containing protein (RefSeq), at 735,195 to 736,091
_0646
Shew_0647: Shew_0647 - hypothetical protein (RefSeq), at 736,106 to 736,297
_0647
Position (kb) 733
734
735
736 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 733.265 kb on - strand, within Shew_0644 at 733.371 kb on + strand, within Shew_0644 at 733.469 kb on + strand, within Shew_0644 at 733.541 kb on - strand, within Shew_0644 at 733.884 kb on + strand at 733.987 kb on - strand at 733.987 kb on - strand at 734.132 kb on - strand, within Shew_0645 at 734.132 kb on - strand, within Shew_0645 at 734.690 kb on + strand, within Shew_0645 at 734.931 kb on + strand, within Shew_0645 at 735.233 kb on - strand at 735.270 kb on + strand at 735.539 kb on + strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.875 kb on - strand, within Shew_0646 at 735.952 kb on + strand, within Shew_0646 at 736.189 kb on - strand, within Shew_0647
Per-strain Table
Position Strand Gene LocusTag Fraction Anaero. fumarate as E acceptor remove 733,265 - Shew_0644 0.64 +0.6 733,371 + Shew_0644 0.70 +0.8 733,469 + Shew_0644 0.76 +0.5 733,541 - Shew_0644 0.80 -0.3 733,884 + +0.6 733,987 - -1.6 733,987 - -0.8 734,132 - Shew_0645 0.19 -3.9 734,132 - Shew_0645 0.19 -1.7 734,690 + Shew_0645 0.61 +0.2 734,931 + Shew_0645 0.80 +0.2 735,233 - -1.9 735,270 + -0.6 735,539 + Shew_0646 0.38 +1.0 735,875 - Shew_0646 0.76 -1.0 735,875 - Shew_0646 0.76 -0.0 735,952 + Shew_0646 0.84 -0.3 736,189 - Shew_0647 0.43 +0.8
Or see this region's nucleotide sequence