Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02475 and MMP_RS02480 are separated by 98 nucleotides MMP_RS02480 and MMP_RS02485 are separated by 2 nucleotides MMP_RS02485 and MMP_RS02490 overlap by 14 nucleotides MMP_RS02490 and MMP_RS02495 are separated by 11 nucleotides MMP_RS02495 and MMP_RS02500 are separated by 1 nucleotides
MMP_RS02475: MMP_RS02475 - hypothetical protein, at 464,129 to 464,380
_RS02475
MMP_RS02480: MMP_RS02480 - hypothetical protein, at 464,479 to 464,997
_RS02480
MMP_RS02485: MMP_RS02485 - DUF2080 family transposase-associated protein, at 465,000 to 465,224
_RS02485
MMP_RS02490: MMP_RS02490 - hypothetical protein, at 465,211 to 465,405
_RS02490
MMP_RS02495: MMP_RS02495 - hypothetical protein, at 465,417 to 465,800
_RS02495
MMP_RS02500: MMP_RS02500 - minichromosome maintenance protein MCM, at 465,802 to 467,826
_RS02500
Position (kb) 465
466 Strain fitness (log2 ratio) -1
0
1 at 464.236 kb on - strand, within MMP_RS02475 at 464.364 kb on + strand at 464.455 kb on - strand at 464.676 kb on + strand, within MMP_RS02480 at 464.825 kb on - strand, within MMP_RS02480 at 465.306 kb on - strand, within MMP_RS02490 at 465.306 kb on - strand, within MMP_RS02490 at 465.307 kb on + strand, within MMP_RS02490 at 465.432 kb on - strand at 465.679 kb on + strand, within MMP_RS02495 at 465.682 kb on - strand, within MMP_RS02495 at 465.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 464,236 - MMP_RS02475 0.42 +0.8 464,364 + +0.3 464,455 - -0.2 464,676 + MMP_RS02480 0.38 +0.7 464,825 - MMP_RS02480 0.67 +0.1 465,306 - MMP_RS02490 0.49 +0.4 465,306 - MMP_RS02490 0.49 -0.2 465,307 + MMP_RS02490 0.49 -0.1 465,432 - -0.1 465,679 + MMP_RS02495 0.68 +0.2 465,682 - MMP_RS02495 0.69 -0.2 465,851 - +0.8
Or see this region's nucleotide sequence