Experiment: MinMedia 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3311 and Shew_3312 are separated by 30 nucleotides Shew_3312 and Shew_3313 are separated by 2 nucleotides Shew_3313 and Shew_3314 overlap by 4 nucleotides Shew_3314 and Shew_3315 overlap by 8 nucleotides
Shew_3311: Shew_3311 - RNA polymerase factor sigma-54 (RefSeq), at 3,951,593 to 3,953,080
_3311
Shew_3312: Shew_3312 - sigma 54 modulation protein/ribosomal protein S30EA (RefSeq), at 3,953,111 to 3,953,398
_3312
Shew_3313: Shew_3313 - PTS IIA-like nitrogen-regulatory protein PtsN (RefSeq), at 3,953,401 to 3,953,844
_3313
Shew_3314: Shew_3314 - hypothetical protein (RefSeq), at 3,953,841 to 3,954,689
_3314
Shew_3315: Shew_3315 - phosphotransferase system, phosphocarrier protein HPr (RefSeq), at 3,954,682 to 3,954,957
_3315
Position (kb) 3953
3954 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 3952.738 kb on + strand, within Shew_3311 at 3953.051 kb on - strand at 3953.217 kb on - strand, within Shew_3312 at 3953.569 kb on + strand, within Shew_3313 at 3953.569 kb on + strand, within Shew_3313 at 3954.150 kb on - strand, within Shew_3314 at 3954.661 kb on - strand at 3954.696 kb on - strand at 3954.761 kb on - strand, within Shew_3315
Per-strain Table
Position Strand Gene LocusTag Fraction MinMedia 30C remove 3,952,738 + Shew_3311 0.77 -4.4 3,953,051 - -3.2 3,953,217 - Shew_3312 0.37 -3.9 3,953,569 + Shew_3313 0.38 -2.1 3,953,569 + Shew_3313 0.38 -3.3 3,954,150 - Shew_3314 0.36 -2.0 3,954,661 - -0.7 3,954,696 - -0.1 3,954,761 - Shew_3315 0.29 -0.5
Or see this region's nucleotide sequence