Experiment: MinMedia 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2764 and Shew_2765 are separated by 182 nucleotides Shew_2765 and Shew_2766 are separated by 371 nucleotides Shew_2766 and motB are separated by 254 nucleotides
Shew_2764: Shew_2764 - DNA-binding transcriptional activator GcvA (RefSeq), at 3,269,102 to 3,270,013
_2764
Shew_2765: Shew_2765 - hypothetical protein (RefSeq), at 3,270,196 to 3,270,426
_2765
Shew_2766: Shew_2766 - thiamine biosynthesis protein ThiI (RefSeq), at 3,270,798 to 3,272,255
_2766
Shew_2767: motB - flagellar motor protein MotB (RefSeq), at 3,272,510 to 3,273,454
motB
Position (kb)
3270
3271
3272
3273 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3270.528 kb on + strand at 3270.528 kb on + strand at 3270.528 kb on + strand at 3270.536 kb on - strand at 3270.987 kb on + strand, within Shew_2766 at 3271.109 kb on + strand, within Shew_2766 at 3271.134 kb on + strand, within Shew_2766 at 3271.797 kb on - strand, within Shew_2766 at 3272.128 kb on + strand at 3272.530 kb on + strand at 3272.799 kb on + strand, within motB at 3272.799 kb on + strand, within motB at 3272.799 kb on + strand, within motB at 3272.807 kb on - strand, within motB at 3272.846 kb on - strand, within motB
Per-strain Table
Position Strand Gene LocusTag Fraction MinMedia 30C remove 3,270,528 + -0.2 3,270,528 + -0.6 3,270,528 + -0.6 3,270,536 - -0.2 3,270,987 + Shew_2766 0.13 -4.5 3,271,109 + Shew_2766 0.21 -3.1 3,271,134 + Shew_2766 0.23 -2.6 3,271,797 - Shew_2766 0.69 -4.6 3,272,128 + -4.3 3,272,530 + +1.1 3,272,799 + motB Shew_2767 0.31 +1.8 3,272,799 + motB Shew_2767 0.31 +2.0 3,272,799 + motB Shew_2767 0.31 +1.7 3,272,807 - motB Shew_2767 0.31 +1.6 3,272,846 - motB Shew_2767 0.36 +1.9
Or see this region's nucleotide sequence