Experiment: MinMedia 30C
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cobT and Shew_0715 are separated by 41 nucleotides Shew_0715 and Shew_0716 overlap by 4 nucleotides Shew_0716 and Shew_0717 are separated by 12 nucleotides
Shew_0714: cobT - nicotinate-nucleotide--dimethylbenzimidazolephosphoribosyltransferase (RefSeq), at 814,495 to 815,523
cobT
Shew_0715: Shew_0715 - cobalamin 5'-phosphate synthase (RefSeq), at 815,565 to 816,332
_0715
Shew_0716: Shew_0716 - cobalbumin biosynthesis enzyme (RefSeq), at 816,329 to 816,877
_0716
Shew_0717: Shew_0717 - cobyric acid synthase (RefSeq), at 816,890 to 818,494
_0717
Position (kb) 815
816
817 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 814.781 kb on - strand, within cobT at 814.879 kb on + strand, within cobT at 815.578 kb on + strand at 815.720 kb on + strand, within Shew_0715 at 815.728 kb on - strand, within Shew_0715 at 815.944 kb on - strand, within Shew_0715 at 816.002 kb on - strand, within Shew_0715 at 816.079 kb on - strand, within Shew_0715 at 816.304 kb on - strand at 816.411 kb on + strand, within Shew_0716 at 816.485 kb on + strand, within Shew_0716 at 816.518 kb on + strand, within Shew_0716 at 816.526 kb on - strand, within Shew_0716 at 816.849 kb on - strand at 817.159 kb on + strand, within Shew_0717 at 817.206 kb on - strand, within Shew_0717 at 817.248 kb on - strand, within Shew_0717
Per-strain Table
Position Strand Gene LocusTag Fraction MinMedia 30C remove 814,781 - cobT Shew_0714 0.28 -2.2 814,879 + cobT Shew_0714 0.37 -0.2 815,578 + -0.2 815,720 + Shew_0715 0.20 -0.7 815,728 - Shew_0715 0.21 -3.5 815,944 - Shew_0715 0.49 -2.8 816,002 - Shew_0715 0.57 -4.4 816,079 - Shew_0715 0.67 -4.0 816,304 - -3.5 816,411 + Shew_0716 0.15 -0.2 816,485 + Shew_0716 0.28 -0.2 816,518 + Shew_0716 0.34 +0.1 816,526 - Shew_0716 0.36 -1.4 816,849 - -5.7 817,159 + Shew_0717 0.17 +0.1 817,206 - Shew_0717 0.20 -4.9 817,248 - Shew_0717 0.22 -3.8
Or see this region's nucleotide sequence