Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3319 and Dshi_3320 are separated by 13 nucleotides Dshi_3320 and Dshi_3321 are separated by 197 nucleotides Dshi_3321 and Dshi_3322 overlap by 4 nucleotides
Dshi_3319: Dshi_3319 - metallophosphoesterase (RefSeq), at 3,482,662 to 3,483,396
_3319
Dshi_3320: Dshi_3320 - hypothetical protein (RefSeq), at 3,483,410 to 3,483,616
_3320
Dshi_3321: Dshi_3321 - CreA family protein (RefSeq), at 3,483,814 to 3,484,269
_3321
Dshi_3322: Dshi_3322 - transcriptional regulator, LacI family (RefSeq), at 3,484,266 to 3,485,303
_3322
Position (kb)
3483
3484
3485 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3483.120 kb on - strand, within Dshi_3319 at 3483.165 kb on + strand, within Dshi_3319 at 3483.175 kb on - strand, within Dshi_3319 at 3483.250 kb on - strand, within Dshi_3319 at 3483.259 kb on + strand, within Dshi_3319 at 3483.341 kb on - strand at 3483.341 kb on - strand at 3483.416 kb on - strand at 3483.555 kb on - strand, within Dshi_3320 at 3483.659 kb on + strand at 3483.659 kb on + strand at 3483.667 kb on - strand at 3483.667 kb on - strand at 3483.667 kb on - strand at 3483.674 kb on + strand at 3483.680 kb on + strand at 3483.682 kb on - strand at 3483.682 kb on - strand at 3483.688 kb on + strand at 3483.842 kb on + strand at 3483.880 kb on - strand, within Dshi_3321 at 3483.955 kb on + strand, within Dshi_3321 at 3483.965 kb on + strand, within Dshi_3321 at 3484.103 kb on + strand, within Dshi_3321 at 3484.257 kb on + strand at 3484.445 kb on - strand, within Dshi_3322 at 3484.483 kb on + strand, within Dshi_3322 at 3484.499 kb on - strand, within Dshi_3322 at 3484.529 kb on + strand, within Dshi_3322 at 3484.574 kb on - strand, within Dshi_3322 at 3484.689 kb on - strand, within Dshi_3322 at 3484.732 kb on - strand, within Dshi_3322 at 3484.781 kb on - strand, within Dshi_3322 at 3484.885 kb on + strand, within Dshi_3322 at 3484.893 kb on - strand, within Dshi_3322 at 3484.932 kb on + strand, within Dshi_3322 at 3485.157 kb on + strand, within Dshi_3322 at 3485.165 kb on - strand, within Dshi_3322 at 3485.167 kb on - strand, within Dshi_3322 at 3485.227 kb on - strand at 3485.234 kb on + strand at 3485.234 kb on + strand at 3485.242 kb on - strand at 3485.263 kb on + strand at 3485.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 3,483,120 - Dshi_3319 0.62 -1.5 3,483,165 + Dshi_3319 0.68 +1.5 3,483,175 - Dshi_3319 0.70 +0.1 3,483,250 - Dshi_3319 0.80 -0.6 3,483,259 + Dshi_3319 0.81 -0.1 3,483,341 - +0.8 3,483,341 - -0.8 3,483,416 - -1.2 3,483,555 - Dshi_3320 0.70 +0.5 3,483,659 + -1.4 3,483,659 + -0.9 3,483,667 - -1.3 3,483,667 - -0.4 3,483,667 - -1.0 3,483,674 + -0.8 3,483,680 + -0.5 3,483,682 - -1.0 3,483,682 - -0.0 3,483,688 + -0.5 3,483,842 + -1.3 3,483,880 - Dshi_3321 0.14 +0.1 3,483,955 + Dshi_3321 0.31 +0.5 3,483,965 + Dshi_3321 0.33 +0.3 3,484,103 + Dshi_3321 0.63 +0.8 3,484,257 + -1.5 3,484,445 - Dshi_3322 0.17 -1.3 3,484,483 + Dshi_3322 0.21 -0.5 3,484,499 - Dshi_3322 0.22 +1.1 3,484,529 + Dshi_3322 0.25 -0.1 3,484,574 - Dshi_3322 0.30 -1.0 3,484,689 - Dshi_3322 0.41 -0.7 3,484,732 - Dshi_3322 0.45 -3.5 3,484,781 - Dshi_3322 0.50 -0.3 3,484,885 + Dshi_3322 0.60 +0.8 3,484,893 - Dshi_3322 0.60 -2.9 3,484,932 + Dshi_3322 0.64 +0.7 3,485,157 + Dshi_3322 0.86 +0.1 3,485,165 - Dshi_3322 0.87 -0.8 3,485,167 - Dshi_3322 0.87 -2.3 3,485,227 - -1.3 3,485,234 + -0.5 3,485,234 + -0.1 3,485,242 - -0.7 3,485,263 + +0.2 3,485,269 - -0.2
Or see this region's nucleotide sequence