Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3046 and Dshi_3047 overlap by 4 nucleotides Dshi_3047 and Dshi_3048 are separated by 120 nucleotides Dshi_3048 and Dshi_3049 are separated by 3 nucleotides
Dshi_3046: Dshi_3046 - Hydroxypyruvate reductase (RefSeq), at 3,214,330 to 3,215,577
_3046
Dshi_3047: Dshi_3047 - 6-phosphogluconate dehydrogenase NAD-binding (RefSeq), at 3,215,574 to 3,216,458
_3047
Dshi_3048: Dshi_3048 - GntR domain protein (RefSeq), at 3,216,579 to 3,217,361
_3048
Dshi_3049: Dshi_3049 - NAD-dependent epimerase/dehydratase (RefSeq), at 3,217,365 to 3,218,309
_3049
Position (kb)
3215
3216
3217 Strain fitness (log2 ratio)
-1
0
1
2 at 3214.892 kb on + strand, within Dshi_3046 at 3215.072 kb on - strand, within Dshi_3046 at 3215.332 kb on - strand, within Dshi_3046 at 3215.389 kb on + strand, within Dshi_3046 at 3215.534 kb on + strand at 3215.615 kb on + strand at 3215.615 kb on + strand at 3215.623 kb on - strand at 3215.623 kb on - strand at 3215.707 kb on + strand, within Dshi_3047 at 3215.707 kb on + strand, within Dshi_3047 at 3215.715 kb on - strand, within Dshi_3047 at 3215.981 kb on - strand, within Dshi_3047 at 3215.988 kb on - strand, within Dshi_3047 at 3216.020 kb on + strand, within Dshi_3047 at 3216.181 kb on + strand, within Dshi_3047 at 3216.311 kb on + strand, within Dshi_3047 at 3216.366 kb on + strand, within Dshi_3047 at 3216.428 kb on + strand at 3216.727 kb on + strand, within Dshi_3048 at 3216.745 kb on + strand, within Dshi_3048 at 3216.946 kb on + strand, within Dshi_3048 at 3216.954 kb on - strand, within Dshi_3048 at 3216.973 kb on - strand, within Dshi_3048 at 3217.076 kb on - strand, within Dshi_3048 at 3217.242 kb on - strand, within Dshi_3048 at 3217.270 kb on + strand, within Dshi_3048
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 3,214,892 + Dshi_3046 0.45 +2.0 3,215,072 - Dshi_3046 0.59 -0.3 3,215,332 - Dshi_3046 0.80 +0.3 3,215,389 + Dshi_3046 0.85 +0.2 3,215,534 + -1.3 3,215,615 + +1.7 3,215,615 + +0.0 3,215,623 - -1.2 3,215,623 - -0.8 3,215,707 + Dshi_3047 0.15 -1.6 3,215,707 + Dshi_3047 0.15 +0.6 3,215,715 - Dshi_3047 0.16 +0.3 3,215,981 - Dshi_3047 0.46 -1.1 3,215,988 - Dshi_3047 0.47 +0.5 3,216,020 + Dshi_3047 0.50 -0.4 3,216,181 + Dshi_3047 0.69 -0.5 3,216,311 + Dshi_3047 0.83 +0.1 3,216,366 + Dshi_3047 0.89 +0.7 3,216,428 + -0.8 3,216,727 + Dshi_3048 0.19 +1.3 3,216,745 + Dshi_3048 0.21 -0.5 3,216,946 + Dshi_3048 0.47 +0.4 3,216,954 - Dshi_3048 0.48 -0.1 3,216,973 - Dshi_3048 0.50 -0.0 3,217,076 - Dshi_3048 0.63 +1.4 3,217,242 - Dshi_3048 0.85 -0.2 3,217,270 + Dshi_3048 0.88 +0.9
Or see this region's nucleotide sequence