Experiment: Thymine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0012 and Dshi_0013 overlap by 4 nucleotides Dshi_0013 and Dshi_0014 overlap by 1 nucleotides Dshi_0014 and Dshi_0015 are separated by 200 nucleotides
Dshi_0012: Dshi_0012 - succinyl-diaminopimelate desuccinylase (RefSeq), at 13,129 to 14,268
_0012
Dshi_0013: Dshi_0013 - GCN5-related N-acetyltransferase (RefSeq), at 14,265 to 14,693
_0013
Dshi_0014: Dshi_0014 - hypothetical protein (RefSeq), at 14,693 to 15,271
_0014
Dshi_0015: Dshi_0015 - ribonuclease R (RefSeq), at 15,472 to 17,727
_0015
Position (kb) 14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 14.307 kb on - strand at 14.342 kb on + strand, within Dshi_0013 at 14.528 kb on - strand, within Dshi_0013 at 14.591 kb on + strand, within Dshi_0013 at 14.624 kb on - strand, within Dshi_0013 at 14.729 kb on + strand at 14.783 kb on + strand, within Dshi_0014 at 14.849 kb on - strand, within Dshi_0014 at 14.898 kb on - strand, within Dshi_0014 at 14.967 kb on + strand, within Dshi_0014 at 15.068 kb on + strand, within Dshi_0014 at 15.076 kb on - strand, within Dshi_0014 at 15.140 kb on + strand, within Dshi_0014 at 15.292 kb on - strand at 15.324 kb on + strand at 15.390 kb on + strand at 15.398 kb on - strand at 15.399 kb on - strand at 15.616 kb on + strand at 15.616 kb on + strand at 15.658 kb on + strand at 15.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thymine (N) remove 14,307 - -0.8 14,342 + Dshi_0013 0.18 -0.4 14,528 - Dshi_0013 0.61 -2.3 14,591 + Dshi_0013 0.76 -0.7 14,624 - Dshi_0013 0.84 -1.1 14,729 + -0.5 14,783 + Dshi_0014 0.16 -0.2 14,849 - Dshi_0014 0.27 +0.1 14,898 - Dshi_0014 0.35 +0.3 14,967 + Dshi_0014 0.47 +0.6 15,068 + Dshi_0014 0.65 +0.5 15,076 - Dshi_0014 0.66 +0.9 15,140 + Dshi_0014 0.77 -0.1 15,292 - -1.1 15,324 + +0.2 15,390 + -1.9 15,398 - +0.4 15,399 - -0.4 15,616 + +0.8 15,616 + +1.3 15,658 + +0.5 15,666 - -0.2
Or see this region's nucleotide sequence