Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07395 and MMP_RS09050 are separated by 487 nucleotides MMP_RS09050 and MMP_RS09055 are separated by 267 nucleotides MMP_RS09055 and MMP_RS07405 are separated by 151 nucleotides
MMP_RS07395: MMP_RS07395 - DNA topoisomerase IV subunit A, at 1,407,876 to 1,408,973
_RS07395
MMP_RS09050: MMP_RS09050 - CAP domain-containing protein, at 1,409,461 to 1,409,646
_RS09050
MMP_RS09055: MMP_RS09055 - CAP domain-containing protein, at 1,409,914 to 1,410,327
_RS09055
MMP_RS07405: MMP_RS07405 - 2 3-bisphosphoglycerate-independent phosphoglycerate mutase, at 1,410,479 to 1,411,699
_RS07405
Position (kb)
1409
1410
1411 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1408.994 kb on + strand at 1409.049 kb on + strand at 1409.331 kb on + strand at 1409.388 kb on - strand at 1409.388 kb on - strand at 1409.395 kb on - strand at 1409.395 kb on - strand at 1409.396 kb on + strand at 1409.447 kb on + strand at 1409.460 kb on - strand at 1409.485 kb on + strand, within MMP_RS09050 at 1409.508 kb on - strand, within MMP_RS09050 at 1409.532 kb on - strand, within MMP_RS09050 at 1409.703 kb on - strand at 1409.777 kb on - strand at 1409.868 kb on + strand at 1409.895 kb on - strand at 1409.895 kb on - strand at 1409.896 kb on + strand at 1409.896 kb on + strand at 1409.896 kb on + strand at 1409.907 kb on - strand at 1409.907 kb on - strand at 1409.908 kb on + strand at 1409.908 kb on + strand at 1409.908 kb on + strand at 1409.911 kb on + strand at 1409.949 kb on + strand at 1409.949 kb on + strand at 1410.024 kb on + strand, within MMP_RS09055 at 1410.298 kb on - strand at 1410.299 kb on + strand at 1410.299 kb on + strand at 1410.302 kb on + strand at 1410.343 kb on + strand at 1410.379 kb on + strand at 1410.479 kb on + strand at 1410.479 kb on + strand at 1410.501 kb on - strand at 1410.502 kb on + strand at 1410.788 kb on + strand at 1410.793 kb on + strand at 1411.131 kb on - strand at 1411.131 kb on - strand at 1411.131 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,408,994 + +2.4 1,409,049 + +2.5 1,409,331 + -1.3 1,409,388 - +0.2 1,409,388 - -1.0 1,409,395 - +0.9 1,409,395 - +0.7 1,409,396 + -1.1 1,409,447 + -1.3 1,409,460 - -0.1 1,409,485 + MMP_RS09050 0.13 +0.3 1,409,508 - MMP_RS09050 0.25 -2.1 1,409,532 - MMP_RS09050 0.38 +0.6 1,409,703 - +0.1 1,409,777 - -1.0 1,409,868 + +0.0 1,409,895 - +0.4 1,409,895 - -1.7 1,409,896 + +1.2 1,409,896 + -2.1 1,409,896 + +0.2 1,409,907 - -0.3 1,409,907 - -0.6 1,409,908 + -1.4 1,409,908 + +1.6 1,409,908 + -0.4 1,409,911 + +0.2 1,409,949 + +0.4 1,409,949 + +0.5 1,410,024 + MMP_RS09055 0.27 -4.0 1,410,298 - +0.4 1,410,299 + +0.3 1,410,299 + -1.7 1,410,302 + +1.9 1,410,343 + -0.1 1,410,379 + +1.6 1,410,479 + +0.2 1,410,479 + -0.5 1,410,501 - -0.6 1,410,502 + -1.4 1,410,788 + -0.5 1,410,793 + -1.4 1,411,131 - -0.9 1,411,131 - -0.9 1,411,131 - -0.8
Or see this region's nucleotide sequence