Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08100 and MMP_RS08105 are separated by 8 nucleotides MMP_RS08105 and MMP_RS09035 are separated by 186 nucleotides MMP_RS09035 and MMP_RS08110 are separated by 15 nucleotides
MMP_RS08100: MMP_RS08100 - 8-amino-7-oxononanoate synthase, at 1,524,069 to 1,525,181
_RS08100
MMP_RS08105: MMP_RS08105 - 6-carboxyhexanoate--CoA ligase, at 1,525,190 to 1,525,924
_RS08105
MMP_RS09035: MMP_RS09035 - hypothetical protein, at 1,526,111 to 1,526,266
_RS09035
MMP_RS08110: MMP_RS08110 - tRNA uridine(34) 5-carboxymethylaminomethyl modification radical SAM/GNAT enzyme Elp3, at 1,526,282 to 1,527,904
_RS08110
Position (kb)
1525
1526 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1524.254 kb on - strand, within MMP_RS08100 at 1524.424 kb on - strand, within MMP_RS08100 at 1524.428 kb on - strand, within MMP_RS08100 at 1524.443 kb on - strand, within MMP_RS08100 at 1524.550 kb on + strand, within MMP_RS08100 at 1524.631 kb on - strand, within MMP_RS08100 at 1524.732 kb on + strand, within MMP_RS08100 at 1524.748 kb on + strand, within MMP_RS08100 at 1524.915 kb on - strand, within MMP_RS08100 at 1525.392 kb on + strand, within MMP_RS08105 at 1525.437 kb on + strand, within MMP_RS08105 at 1525.544 kb on - strand, within MMP_RS08105 at 1525.565 kb on + strand, within MMP_RS08105 at 1525.565 kb on + strand, within MMP_RS08105 at 1525.585 kb on - strand, within MMP_RS08105 at 1525.602 kb on + strand, within MMP_RS08105 at 1525.621 kb on - strand, within MMP_RS08105 at 1525.673 kb on - strand, within MMP_RS08105 at 1525.722 kb on + strand, within MMP_RS08105 at 1526.061 kb on + strand at 1526.064 kb on - strand at 1526.066 kb on - strand at 1526.067 kb on + strand at 1526.069 kb on + strand at 1526.069 kb on + strand at 1526.085 kb on + strand at 1526.119 kb on - strand at 1526.129 kb on + strand, within MMP_RS09035 at 1526.235 kb on - strand, within MMP_RS09035 at 1526.236 kb on + strand, within MMP_RS09035 at 1526.242 kb on - strand, within MMP_RS09035 at 1526.243 kb on + strand, within MMP_RS09035 at 1526.254 kb on + strand at 1526.269 kb on - strand at 1526.283 kb on - strand at 1526.337 kb on - strand at 1526.338 kb on + strand at 1526.341 kb on + strand at 1526.563 kb on - strand at 1526.571 kb on + strand at 1526.882 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,524,254 - MMP_RS08100 0.17 -0.8 1,524,424 - MMP_RS08100 0.32 +0.4 1,524,428 - MMP_RS08100 0.32 +0.0 1,524,443 - MMP_RS08100 0.34 -1.1 1,524,550 + MMP_RS08100 0.43 +1.3 1,524,631 - MMP_RS08100 0.50 +1.1 1,524,732 + MMP_RS08100 0.60 +0.2 1,524,748 + MMP_RS08100 0.61 -0.9 1,524,915 - MMP_RS08100 0.76 -3.7 1,525,392 + MMP_RS08105 0.27 +0.4 1,525,437 + MMP_RS08105 0.34 +1.4 1,525,544 - MMP_RS08105 0.48 -0.9 1,525,565 + MMP_RS08105 0.51 -0.7 1,525,565 + MMP_RS08105 0.51 -1.8 1,525,585 - MMP_RS08105 0.54 -0.4 1,525,602 + MMP_RS08105 0.56 -1.2 1,525,621 - MMP_RS08105 0.59 -2.5 1,525,673 - MMP_RS08105 0.66 -0.4 1,525,722 + MMP_RS08105 0.72 -1.3 1,526,061 + +1.0 1,526,064 - +0.1 1,526,066 - +1.0 1,526,067 + +1.3 1,526,069 + +0.6 1,526,069 + -2.3 1,526,085 + +0.8 1,526,119 - +0.4 1,526,129 + MMP_RS09035 0.12 -1.6 1,526,235 - MMP_RS09035 0.79 +1.6 1,526,236 + MMP_RS09035 0.80 -3.1 1,526,242 - MMP_RS09035 0.84 +0.1 1,526,243 + MMP_RS09035 0.85 +2.1 1,526,254 + -2.0 1,526,269 - +0.9 1,526,283 - +1.4 1,526,337 - -3.0 1,526,338 + +0.7 1,526,341 + -2.6 1,526,563 - -2.2 1,526,571 + -2.3 1,526,882 + -2.5
Or see this region's nucleotide sequence