Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08065 and MMP_RS08070 are separated by 86 nucleotides MMP_RS08070 and MMP_RS08075 overlap by 23 nucleotides MMP_RS08075 and MMP_RS08080 are separated by 10 nucleotides MMP_RS08080 and MMP_RS08085 are separated by 8 nucleotides
MMP_RS08065: MMP_RS08065 - tetrahydromethanopterin S-methyltransferase subunit H, at 1,519,462 to 1,520,421
_RS08065
MMP_RS08070: MMP_RS08070 - DUF2096 domain-containing protein, at 1,520,508 to 1,521,041
_RS08070
MMP_RS08075: MMP_RS08075 - DUF749 domain-containing protein, at 1,521,019 to 1,521,327
_RS08075
MMP_RS08080: MMP_RS08080 - YchF/TatD family DNA exonuclease, at 1,521,338 to 1,522,093
_RS08080
MMP_RS08085: MMP_RS08085 - hypothetical protein, at 1,522,102 to 1,523,037
_RS08085
Position (kb)
1521
1522 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1520.424 kb on + strand at 1520.488 kb on - strand at 1520.488 kb on - strand at 1520.488 kb on - strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.510 kb on + strand at 1520.559 kb on - strand at 1520.639 kb on - strand, within MMP_RS08070 at 1520.670 kb on - strand, within MMP_RS08070 at 1520.727 kb on + strand, within MMP_RS08070 at 1520.838 kb on - strand, within MMP_RS08070 at 1520.909 kb on + strand, within MMP_RS08070 at 1520.912 kb on + strand, within MMP_RS08070 at 1520.915 kb on - strand, within MMP_RS08070 at 1521.003 kb on - strand at 1521.004 kb on + strand at 1521.004 kb on + strand at 1521.151 kb on - strand, within MMP_RS08075 at 1521.152 kb on + strand, within MMP_RS08075 at 1521.158 kb on - strand, within MMP_RS08075 at 1521.186 kb on - strand, within MMP_RS08075 at 1521.190 kb on + strand, within MMP_RS08075 at 1521.348 kb on - strand at 1521.350 kb on - strand at 1521.369 kb on - strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.373 kb on - strand at 1521.493 kb on - strand, within MMP_RS08080 at 1521.493 kb on - strand, within MMP_RS08080 at 1521.564 kb on + strand, within MMP_RS08080 at 1521.572 kb on + strand, within MMP_RS08080 at 1521.577 kb on + strand, within MMP_RS08080 at 1521.755 kb on - strand, within MMP_RS08080 at 1521.849 kb on - strand, within MMP_RS08080 at 1521.868 kb on - strand, within MMP_RS08080 at 1521.881 kb on + strand, within MMP_RS08080 at 1521.909 kb on + strand, within MMP_RS08080 at 1521.977 kb on - strand, within MMP_RS08080 at 1522.010 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.071 kb on - strand at 1522.076 kb on + strand at 1522.086 kb on + strand at 1522.123 kb on - strand at 1522.265 kb on + strand, within MMP_RS08085 at 1522.309 kb on - strand, within MMP_RS08085
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,520,424 + +1.0 1,520,488 - +0.7 1,520,488 - +1.2 1,520,488 - -0.5 1,520,489 + +0.4 1,520,489 + +1.0 1,520,489 + +0.1 1,520,489 + -2.1 1,520,489 + -1.6 1,520,489 + +0.6 1,520,489 + -3.8 1,520,510 + +1.1 1,520,559 - +0.1 1,520,639 - MMP_RS08070 0.25 +0.0 1,520,670 - MMP_RS08070 0.30 -0.3 1,520,727 + MMP_RS08070 0.41 +0.8 1,520,838 - MMP_RS08070 0.62 -3.5 1,520,909 + MMP_RS08070 0.75 -1.3 1,520,912 + MMP_RS08070 0.76 -2.1 1,520,915 - MMP_RS08070 0.76 -0.4 1,521,003 - -1.2 1,521,004 + -1.5 1,521,004 + -1.7 1,521,151 - MMP_RS08075 0.43 -0.5 1,521,152 + MMP_RS08075 0.43 +0.0 1,521,158 - MMP_RS08075 0.45 -0.2 1,521,186 - MMP_RS08075 0.54 -1.0 1,521,190 + MMP_RS08075 0.55 -1.6 1,521,348 - +0.3 1,521,350 - -0.2 1,521,369 - -0.8 1,521,370 + +0.6 1,521,370 + -1.2 1,521,370 + +1.6 1,521,373 - -0.5 1,521,493 - MMP_RS08080 0.21 -1.4 1,521,493 - MMP_RS08080 0.21 +1.5 1,521,564 + MMP_RS08080 0.30 +0.9 1,521,572 + MMP_RS08080 0.31 +2.1 1,521,577 + MMP_RS08080 0.32 -1.6 1,521,755 - MMP_RS08080 0.55 -0.8 1,521,849 - MMP_RS08080 0.68 -0.1 1,521,868 - MMP_RS08080 0.70 +0.9 1,521,881 + MMP_RS08080 0.72 +0.3 1,521,909 + MMP_RS08080 0.76 +1.6 1,521,977 - MMP_RS08080 0.85 +0.9 1,522,010 - MMP_RS08080 0.89 +0.2 1,522,015 - MMP_RS08080 0.90 -0.3 1,522,015 - MMP_RS08080 0.90 +0.7 1,522,071 - +0.2 1,522,076 + -0.6 1,522,086 + -0.7 1,522,123 - +0.2 1,522,265 + MMP_RS08085 0.17 +0.6 1,522,309 - MMP_RS08085 0.22 +1.9
Or see this region's nucleotide sequence