Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07690 and MMP_RS07695 are separated by 24 nucleotides MMP_RS07695 and MMP_RS07700 overlap by 4 nucleotides MMP_RS07700 and MMP_RS07705 are separated by 17 nucleotides MMP_RS07705 and MMP_RS07710 are separated by 48 nucleotides
MMP_RS07690: MMP_RS07690 - phenylalanine--tRNA ligase subunit alpha, at 1,457,002 to 1,458,504
_RS07690
MMP_RS07695: MMP_RS07695 - DUF3236 domain-containing protein, at 1,458,529 to 1,459,008
_RS07695
MMP_RS07700: MMP_RS07700 - DUF1188 domain-containing protein, at 1,459,005 to 1,459,787
_RS07700
MMP_RS07705: MMP_RS07705 - transcriptional regulator, at 1,459,805 to 1,460,317
_RS07705
MMP_RS07710: MMP_RS07710 - cell division protein FtsZ, at 1,460,366 to 1,461,463
_RS07710
Position (kb)
1459
1460 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1458.101 kb on - strand at 1458.439 kb on + strand at 1458.630 kb on + strand, within MMP_RS07695 at 1458.654 kb on + strand, within MMP_RS07695 at 1458.683 kb on - strand, within MMP_RS07695 at 1458.789 kb on + strand, within MMP_RS07695 at 1458.818 kb on - strand, within MMP_RS07695 at 1458.824 kb on - strand, within MMP_RS07695 at 1458.941 kb on - strand, within MMP_RS07695 at 1458.942 kb on + strand, within MMP_RS07695 at 1459.019 kb on - strand at 1459.108 kb on + strand, within MMP_RS07700 at 1459.115 kb on + strand, within MMP_RS07700 at 1459.131 kb on + strand, within MMP_RS07700 at 1459.144 kb on - strand, within MMP_RS07700 at 1459.170 kb on - strand, within MMP_RS07700 at 1459.208 kb on - strand, within MMP_RS07700 at 1459.234 kb on - strand, within MMP_RS07700 at 1459.235 kb on + strand, within MMP_RS07700 at 1459.297 kb on - strand, within MMP_RS07700 at 1459.347 kb on + strand, within MMP_RS07700 at 1459.370 kb on + strand, within MMP_RS07700 at 1459.449 kb on + strand, within MMP_RS07700 at 1459.452 kb on + strand, within MMP_RS07700 at 1459.511 kb on - strand, within MMP_RS07700 at 1459.555 kb on - strand, within MMP_RS07700 at 1459.556 kb on + strand, within MMP_RS07700 at 1459.556 kb on + strand, within MMP_RS07700 at 1459.589 kb on + strand, within MMP_RS07700 at 1459.699 kb on - strand, within MMP_RS07700 at 1459.722 kb on - strand at 1459.722 kb on - strand at 1459.763 kb on + strand at 1459.763 kb on + strand at 1459.841 kb on + strand at 1459.861 kb on - strand, within MMP_RS07705 at 1459.907 kb on - strand, within MMP_RS07705 at 1459.908 kb on + strand, within MMP_RS07705 at 1459.954 kb on + strand, within MMP_RS07705 at 1459.956 kb on + strand, within MMP_RS07705 at 1459.984 kb on - strand, within MMP_RS07705 at 1459.985 kb on + strand, within MMP_RS07705 at 1460.055 kb on + strand, within MMP_RS07705 at 1460.122 kb on - strand, within MMP_RS07705 at 1460.182 kb on + strand, within MMP_RS07705 at 1460.188 kb on - strand, within MMP_RS07705 at 1460.188 kb on - strand, within MMP_RS07705 at 1460.189 kb on + strand, within MMP_RS07705 at 1460.192 kb on + strand, within MMP_RS07705 at 1460.192 kb on + strand, within MMP_RS07705 at 1460.208 kb on + strand, within MMP_RS07705 at 1460.208 kb on + strand, within MMP_RS07705 at 1460.294 kb on - strand at 1460.294 kb on - strand at 1460.294 kb on - strand at 1460.317 kb on - strand at 1460.328 kb on - strand at 1460.359 kb on + strand at 1460.369 kb on - strand at 1460.374 kb on - strand at 1460.377 kb on + strand at 1460.467 kb on - strand at 1460.490 kb on + strand, within MMP_RS07710 at 1460.536 kb on - strand, within MMP_RS07710 at 1460.550 kb on + strand, within MMP_RS07710 at 1460.566 kb on - strand, within MMP_RS07710 at 1460.587 kb on - strand, within MMP_RS07710 at 1460.664 kb on + strand, within MMP_RS07710 at 1460.679 kb on + strand, within MMP_RS07710 at 1460.683 kb on - strand, within MMP_RS07710 at 1460.694 kb on + strand, within MMP_RS07710 at 1460.717 kb on + strand, within MMP_RS07710
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,458,101 - +0.4 1,458,439 + -0.6 1,458,630 + MMP_RS07695 0.21 -1.7 1,458,654 + MMP_RS07695 0.26 +1.3 1,458,683 - MMP_RS07695 0.32 +0.0 1,458,789 + MMP_RS07695 0.54 +0.5 1,458,818 - MMP_RS07695 0.60 -3.7 1,458,824 - MMP_RS07695 0.61 -1.3 1,458,941 - MMP_RS07695 0.86 +0.0 1,458,942 + MMP_RS07695 0.86 -0.6 1,459,019 - -3.1 1,459,108 + MMP_RS07700 0.13 -0.2 1,459,115 + MMP_RS07700 0.14 -2.3 1,459,131 + MMP_RS07700 0.16 -2.5 1,459,144 - MMP_RS07700 0.18 -1.0 1,459,170 - MMP_RS07700 0.21 -3.7 1,459,208 - MMP_RS07700 0.26 -1.5 1,459,234 - MMP_RS07700 0.29 +0.3 1,459,235 + MMP_RS07700 0.29 +0.1 1,459,297 - MMP_RS07700 0.37 -1.3 1,459,347 + MMP_RS07700 0.44 +0.9 1,459,370 + MMP_RS07700 0.47 -3.0 1,459,449 + MMP_RS07700 0.57 +0.4 1,459,452 + MMP_RS07700 0.57 -3.9 1,459,511 - MMP_RS07700 0.65 -0.2 1,459,555 - MMP_RS07700 0.70 -0.6 1,459,556 + MMP_RS07700 0.70 -0.5 1,459,556 + MMP_RS07700 0.70 -1.5 1,459,589 + MMP_RS07700 0.75 -3.3 1,459,699 - MMP_RS07700 0.89 +0.1 1,459,722 - -0.7 1,459,722 - +1.3 1,459,763 + -1.2 1,459,763 + -1.5 1,459,841 + +1.7 1,459,861 - MMP_RS07705 0.11 -1.9 1,459,907 - MMP_RS07705 0.20 -1.3 1,459,908 + MMP_RS07705 0.20 +1.1 1,459,954 + MMP_RS07705 0.29 +0.0 1,459,956 + MMP_RS07705 0.29 -2.3 1,459,984 - MMP_RS07705 0.35 -1.6 1,459,985 + MMP_RS07705 0.35 +1.1 1,460,055 + MMP_RS07705 0.49 -2.0 1,460,122 - MMP_RS07705 0.62 +0.9 1,460,182 + MMP_RS07705 0.73 -3.0 1,460,188 - MMP_RS07705 0.75 +0.3 1,460,188 - MMP_RS07705 0.75 -2.3 1,460,189 + MMP_RS07705 0.75 -1.8 1,460,192 + MMP_RS07705 0.75 -2.7 1,460,192 + MMP_RS07705 0.75 -0.4 1,460,208 + MMP_RS07705 0.79 +2.5 1,460,208 + MMP_RS07705 0.79 -1.3 1,460,294 - +2.2 1,460,294 - +0.9 1,460,294 - -0.8 1,460,317 - -1.3 1,460,328 - -0.3 1,460,359 + -1.5 1,460,369 - -1.7 1,460,374 - +1.3 1,460,377 + -0.5 1,460,467 - -0.4 1,460,490 + MMP_RS07710 0.11 +0.1 1,460,536 - MMP_RS07710 0.15 +1.9 1,460,550 + MMP_RS07710 0.17 -1.8 1,460,566 - MMP_RS07710 0.18 -1.2 1,460,587 - MMP_RS07710 0.20 -0.5 1,460,664 + MMP_RS07710 0.27 +0.5 1,460,679 + MMP_RS07710 0.29 -2.0 1,460,683 - MMP_RS07710 0.29 -1.5 1,460,694 + MMP_RS07710 0.30 +1.0 1,460,717 + MMP_RS07710 0.32 +0.9
Or see this region's nucleotide sequence