Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07520 and MMP_RS07525 are separated by 28 nucleotides MMP_RS07525 and MMP_RS07530 overlap by 8 nucleotides MMP_RS07530 and MMP_RS07535 are separated by 20 nucleotides
MMP_RS07520: MMP_RS07520 - nickel-dependent hydrogenase large subunit, at 1,424,070 to 1,425,197
_RS07520
MMP_RS07525: MMP_RS07525 - 4Fe-4S binding protein, at 1,425,226 to 1,426,407
_RS07525
MMP_RS07530: MMP_RS07530 - 4Fe-4S binding protein, at 1,426,400 to 1,426,885
_RS07530
MMP_RS07535: MMP_RS07535 - 4Fe-4S binding protein, at 1,426,906 to 1,427,664
_RS07535
Position (kb)
1425
1426
1427 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1424.268 kb on - strand at 1424.268 kb on - strand at 1424.345 kb on + strand at 1424.463 kb on + strand at 1424.543 kb on + strand at 1424.946 kb on - strand at 1424.973 kb on - strand at 1425.060 kb on - strand at 1425.060 kb on - strand at 1425.068 kb on + strand at 1425.105 kb on - strand at 1425.105 kb on - strand at 1425.105 kb on - strand at 1425.107 kb on - strand at 1425.180 kb on + strand at 1425.187 kb on + strand at 1425.198 kb on - strand at 1425.203 kb on + strand at 1425.203 kb on + strand at 1425.221 kb on - strand at 1425.225 kb on - strand at 1425.225 kb on - strand at 1425.225 kb on - strand at 1425.225 kb on - strand at 1425.226 kb on + strand at 1425.226 kb on + strand at 1425.226 kb on + strand at 1425.226 kb on + strand at 1425.315 kb on - strand at 1425.372 kb on - strand, within MMP_RS07525 at 1425.479 kb on - strand, within MMP_RS07525 at 1425.587 kb on - strand, within MMP_RS07525 at 1425.588 kb on + strand, within MMP_RS07525 at 1425.591 kb on + strand, within MMP_RS07525 at 1425.854 kb on - strand, within MMP_RS07525 at 1425.945 kb on - strand, within MMP_RS07525 at 1425.946 kb on + strand, within MMP_RS07525 at 1425.961 kb on + strand, within MMP_RS07525 at 1426.004 kb on - strand, within MMP_RS07525 at 1426.051 kb on - strand, within MMP_RS07525 at 1426.095 kb on - strand, within MMP_RS07525 at 1426.095 kb on - strand, within MMP_RS07525 at 1426.105 kb on + strand, within MMP_RS07525 at 1426.131 kb on - strand, within MMP_RS07525 at 1426.219 kb on - strand, within MMP_RS07525 at 1426.325 kb on - strand at 1426.325 kb on - strand at 1426.518 kb on - strand, within MMP_RS07530 at 1426.518 kb on - strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.519 kb on + strand, within MMP_RS07530 at 1426.522 kb on - strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.523 kb on + strand, within MMP_RS07530 at 1426.526 kb on + strand, within MMP_RS07530 at 1426.526 kb on + strand, within MMP_RS07530 at 1426.526 kb on + strand, within MMP_RS07530 at 1426.594 kb on - strand, within MMP_RS07530 at 1426.743 kb on - strand, within MMP_RS07530 at 1426.744 kb on + strand, within MMP_RS07530 at 1426.886 kb on - strand at 1426.947 kb on - strand at 1427.029 kb on - strand, within MMP_RS07535 at 1427.053 kb on + strand, within MMP_RS07535 at 1427.228 kb on - strand, within MMP_RS07535 at 1427.229 kb on + strand, within MMP_RS07535 at 1427.256 kb on + strand, within MMP_RS07535 at 1427.380 kb on + strand, within MMP_RS07535 at 1427.381 kb on - strand, within MMP_RS07535 at 1427.381 kb on - strand, within MMP_RS07535 at 1427.382 kb on + strand, within MMP_RS07535
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,424,268 - -1.0 1,424,268 - -0.7 1,424,345 + -2.6 1,424,463 + -0.3 1,424,543 + -0.2 1,424,946 - -0.2 1,424,973 - -0.4 1,425,060 - -0.2 1,425,060 - -1.8 1,425,068 + -1.8 1,425,105 - -0.9 1,425,105 - -1.3 1,425,105 - -0.9 1,425,107 - -2.7 1,425,180 + -1.5 1,425,187 + -0.3 1,425,198 - -3.2 1,425,203 + -0.3 1,425,203 + -3.6 1,425,221 - -1.8 1,425,225 - -0.9 1,425,225 - -4.1 1,425,225 - -0.8 1,425,225 - -2.4 1,425,226 + -0.0 1,425,226 + -1.5 1,425,226 + +0.1 1,425,226 + -0.8 1,425,315 - -1.9 1,425,372 - MMP_RS07525 0.12 -2.7 1,425,479 - MMP_RS07525 0.21 -3.4 1,425,587 - MMP_RS07525 0.31 -1.6 1,425,588 + MMP_RS07525 0.31 -1.0 1,425,591 + MMP_RS07525 0.31 -2.5 1,425,854 - MMP_RS07525 0.53 -0.8 1,425,945 - MMP_RS07525 0.61 -0.2 1,425,946 + MMP_RS07525 0.61 -1.2 1,425,961 + MMP_RS07525 0.62 -0.8 1,426,004 - MMP_RS07525 0.66 -1.9 1,426,051 - MMP_RS07525 0.70 -0.4 1,426,095 - MMP_RS07525 0.74 -0.0 1,426,095 - MMP_RS07525 0.74 -2.7 1,426,105 + MMP_RS07525 0.74 -1.5 1,426,131 - MMP_RS07525 0.77 -3.4 1,426,219 - MMP_RS07525 0.84 -0.5 1,426,325 - +1.3 1,426,325 - +0.5 1,426,518 - MMP_RS07530 0.24 -0.7 1,426,518 - MMP_RS07530 0.24 +1.2 1,426,519 + MMP_RS07530 0.24 +0.8 1,426,519 + MMP_RS07530 0.24 -2.5 1,426,519 + MMP_RS07530 0.24 +0.9 1,426,519 + MMP_RS07530 0.24 -2.9 1,426,522 - MMP_RS07530 0.25 +0.0 1,426,523 + MMP_RS07530 0.25 +0.1 1,426,523 + MMP_RS07530 0.25 -0.9 1,426,523 + MMP_RS07530 0.25 +0.3 1,426,523 + MMP_RS07530 0.25 +0.0 1,426,523 + MMP_RS07530 0.25 +0.4 1,426,526 + MMP_RS07530 0.26 -1.5 1,426,526 + MMP_RS07530 0.26 +1.2 1,426,526 + MMP_RS07530 0.26 -2.5 1,426,594 - MMP_RS07530 0.40 -0.1 1,426,743 - MMP_RS07530 0.71 -0.3 1,426,744 + MMP_RS07530 0.71 +1.1 1,426,886 - +0.2 1,426,947 - -0.7 1,427,029 - MMP_RS07535 0.16 -0.5 1,427,053 + MMP_RS07535 0.19 -1.4 1,427,228 - MMP_RS07535 0.42 +0.6 1,427,229 + MMP_RS07535 0.43 -0.4 1,427,256 + MMP_RS07535 0.46 +0.7 1,427,380 + MMP_RS07535 0.62 -3.6 1,427,381 - MMP_RS07535 0.63 +0.5 1,427,381 - MMP_RS07535 0.63 -0.2 1,427,382 + MMP_RS07535 0.63 -1.3
Or see this region's nucleotide sequence