Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06770 and MMP_RS06775 are separated by 320 nucleotides MMP_RS06775 and MMP_RS06780 are separated by 9 nucleotides MMP_RS06780 and MMP_RS06785 are separated by 99 nucleotides
MMP_RS06770: MMP_RS06770 - DHH family phosphoesterase, at 1,298,511 to 1,300,796
_RS06770
MMP_RS06775: MMP_RS06775 - 2-oxoacid:ferredoxin oxidoreductase subunit gamma, at 1,301,117 to 1,301,650
_RS06775
MMP_RS06780: MMP_RS06780 - 2-oxoacid:ferredoxin oxidoreductase subunit beta, at 1,301,660 to 1,302,457
_RS06780
MMP_RS06785: MMP_RS06785 - hypothetical protein, at 1,302,557 to 1,303,333
_RS06785
Position (kb)
1301
1302 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1300.150 kb on + strand, within MMP_RS06770 at 1300.461 kb on - strand, within MMP_RS06770 at 1300.623 kb on + strand at 1300.797 kb on + strand at 1300.799 kb on - strand at 1300.836 kb on + strand at 1300.878 kb on - strand at 1300.879 kb on + strand at 1300.881 kb on + strand at 1300.882 kb on - strand at 1300.884 kb on - strand at 1300.884 kb on - strand at 1300.958 kb on + strand at 1300.960 kb on + strand at 1301.108 kb on - strand at 1301.444 kb on + strand at 1301.588 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,300,150 + MMP_RS06770 0.72 -2.0 1,300,461 - MMP_RS06770 0.85 +0.3 1,300,623 + +1.5 1,300,797 + -0.4 1,300,799 - +0.1 1,300,836 + -0.3 1,300,878 - -4.7 1,300,879 + +1.8 1,300,881 + -2.7 1,300,882 - +1.2 1,300,884 - +1.5 1,300,884 - -1.0 1,300,958 + +0.2 1,300,960 + +1.5 1,301,108 - -1.7 1,301,444 + -0.9 1,301,588 - +0.8
Or see this region's nucleotide sequence