Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06745 and MMP_RS06750 are separated by 35 nucleotides MMP_RS06750 and MMP_RS06755 are separated by 29 nucleotides MMP_RS06755 and MMP_RS06760 are separated by 17 nucleotides MMP_RS06760 and MMP_RS06765 are separated by 8 nucleotides
MMP_RS06745: MMP_RS06745 - DUF2119 domain-containing protein, at 1,295,407 to 1,296,018
_RS06745
MMP_RS06750: MMP_RS06750 - IMP cyclohydrolase, at 1,296,054 to 1,296,659
_RS06750
MMP_RS06755: MMP_RS06755 - 4Fe-4S binding protein, at 1,296,689 to 1,296,847
_RS06755
MMP_RS06760: MMP_RS06760 - hypothetical protein, at 1,296,865 to 1,297,491
_RS06760
MMP_RS06765: MMP_RS06765 - flap endonuclease-1, at 1,297,500 to 1,298,474
_RS06765
Position (kb)
1296
1297
1298 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1295.940 kb on + strand, within MMP_RS06745 at 1296.147 kb on - strand at 1296.489 kb on + strand at 1296.655 kb on - strand at 1296.656 kb on + strand at 1296.729 kb on + strand at 1296.824 kb on - strand at 1296.861 kb on - strand at 1296.862 kb on + strand at 1296.919 kb on + strand at 1297.159 kb on + strand, within MMP_RS06760 at 1297.263 kb on - strand, within MMP_RS06760 at 1297.388 kb on + strand, within MMP_RS06760 at 1297.496 kb on + strand at 1297.650 kb on - strand, within MMP_RS06765 at 1297.706 kb on + strand, within MMP_RS06765 at 1297.965 kb on - strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.966 kb on + strand, within MMP_RS06765 at 1297.967 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.034 kb on - strand, within MMP_RS06765 at 1298.035 kb on + strand, within MMP_RS06765 at 1298.038 kb on - strand, within MMP_RS06765 at 1298.411 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,295,940 + MMP_RS06745 0.87 -4.2 1,296,147 - -1.0 1,296,489 + -1.4 1,296,655 - -1.3 1,296,656 + -0.1 1,296,729 + +0.2 1,296,824 - -2.8 1,296,861 - +0.4 1,296,862 + +1.0 1,296,919 + +1.7 1,297,159 + MMP_RS06760 0.47 -1.7 1,297,263 - MMP_RS06760 0.63 -1.7 1,297,388 + MMP_RS06760 0.83 -3.2 1,297,496 + -0.1 1,297,650 - MMP_RS06765 0.15 +0.6 1,297,706 + MMP_RS06765 0.21 +2.5 1,297,965 - MMP_RS06765 0.48 +2.4 1,297,966 + MMP_RS06765 0.48 -1.4 1,297,966 + MMP_RS06765 0.48 +0.3 1,297,967 - MMP_RS06765 0.48 +1.4 1,298,034 - MMP_RS06765 0.55 -1.1 1,298,034 - MMP_RS06765 0.55 -2.6 1,298,035 + MMP_RS06765 0.55 +1.4 1,298,038 - MMP_RS06765 0.55 +0.2 1,298,411 - -0.5
Or see this region's nucleotide sequence