Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06735 and MMP_RS06740 are separated by 51 nucleotides MMP_RS06740 and MMP_RS06745 are separated by 75 nucleotides MMP_RS06745 and MMP_RS06750 are separated by 35 nucleotides
MMP_RS06735: MMP_RS06735 - class I SAM-dependent rRNA methyltransferase, at 1,293,490 to 1,294,632
_RS06735
MMP_RS06740: MMP_RS06740 - fructose-6-phosphate aldolase, at 1,294,684 to 1,295,331
_RS06740
MMP_RS06745: MMP_RS06745 - DUF2119 domain-containing protein, at 1,295,407 to 1,296,018
_RS06745
MMP_RS06750: MMP_RS06750 - IMP cyclohydrolase, at 1,296,054 to 1,296,659
_RS06750
Position (kb)
1294
1295
1296 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1293.856 kb on + strand, within MMP_RS06735 at 1294.295 kb on - strand, within MMP_RS06735 at 1294.300 kb on + strand, within MMP_RS06735 at 1294.308 kb on - strand, within MMP_RS06735 at 1294.508 kb on - strand, within MMP_RS06735 at 1294.509 kb on + strand, within MMP_RS06735 at 1294.552 kb on + strand at 1294.604 kb on - strand at 1294.685 kb on - strand at 1295.089 kb on - strand at 1295.171 kb on + strand at 1295.240 kb on + strand at 1295.370 kb on + strand at 1295.385 kb on - strand at 1295.389 kb on - strand at 1295.390 kb on + strand at 1295.395 kb on + strand at 1295.395 kb on + strand at 1295.468 kb on - strand at 1295.472 kb on - strand, within MMP_RS06745 at 1295.539 kb on - strand, within MMP_RS06745 at 1295.659 kb on - strand, within MMP_RS06745 at 1295.660 kb on + strand, within MMP_RS06745 at 1295.940 kb on + strand, within MMP_RS06745 at 1296.147 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,293,856 + MMP_RS06735 0.32 -0.3 1,294,295 - MMP_RS06735 0.70 +1.6 1,294,300 + MMP_RS06735 0.71 +1.1 1,294,308 - MMP_RS06735 0.72 +0.6 1,294,508 - MMP_RS06735 0.89 -0.8 1,294,509 + MMP_RS06735 0.89 +1.3 1,294,552 + +0.7 1,294,604 - +1.4 1,294,685 - +0.8 1,295,089 - -1.4 1,295,171 + -0.0 1,295,240 + -2.2 1,295,370 + -0.1 1,295,385 - -0.7 1,295,389 - -4.5 1,295,390 + -1.3 1,295,395 + -0.5 1,295,395 + +1.5 1,295,468 - +0.1 1,295,472 - MMP_RS06745 0.11 -1.6 1,295,539 - MMP_RS06745 0.22 +1.5 1,295,659 - MMP_RS06745 0.41 -4.1 1,295,660 + MMP_RS06745 0.41 -0.1 1,295,940 + MMP_RS06745 0.87 -4.2 1,296,147 - -1.0
Or see this region's nucleotide sequence