Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05965 and MMP_RS05970 are separated by 13 nucleotides MMP_RS05970 and MMP_RS05975 are separated by 20 nucleotides MMP_RS05975 and MMP_RS05980 are separated by 22 nucleotides MMP_RS05980 and MMP_RS05985 are separated by 18 nucleotides MMP_RS05985 and MMP_RS05990 are separated by 35 nucleotides MMP_RS05990 and MMP_RS05995 are separated by 38 nucleotides
MMP_RS05965: MMP_RS05965 - carboxymuconolactone decarboxylase family protein, at 1,144,326 to 1,144,640
_RS05965
MMP_RS05970: MMP_RS05970 - class II SORL domain-containing protein, at 1,144,654 to 1,145,010
_RS05970
MMP_RS05975: MMP_RS05975 - hypothetical protein, at 1,145,031 to 1,145,297
_RS05975
MMP_RS05980: MMP_RS05980 - ferritin, at 1,145,320 to 1,145,835
_RS05980
MMP_RS05985: MMP_RS05985 - hypothetical protein, at 1,145,854 to 1,146,042
_RS05985
MMP_RS05990: MMP_RS05990 - DsrE family protein, at 1,146,078 to 1,146,428
_RS05990
MMP_RS05995: MMP_RS05995 - FprA family A-type flavoprotein, at 1,146,467 to 1,147,630
_RS05995
Position (kb)
1145
1146 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1144.544 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.580 kb on + strand, within MMP_RS05965 at 1144.653 kb on + strand at 1144.785 kb on + strand, within MMP_RS05970 at 1144.829 kb on - strand, within MMP_RS05970 at 1144.830 kb on + strand, within MMP_RS05970 at 1144.853 kb on - strand, within MMP_RS05970 at 1145.108 kb on - strand, within MMP_RS05975 at 1145.288 kb on - strand at 1145.292 kb on + strand at 1145.303 kb on + strand at 1145.319 kb on - strand at 1145.319 kb on - strand at 1145.320 kb on + strand at 1145.357 kb on - strand at 1145.357 kb on - strand at 1145.357 kb on - strand at 1145.358 kb on + strand at 1145.358 kb on + strand at 1145.362 kb on + strand at 1145.380 kb on - strand, within MMP_RS05980 at 1145.380 kb on - strand, within MMP_RS05980 at 1145.447 kb on - strand, within MMP_RS05980 at 1145.539 kb on + strand, within MMP_RS05980 at 1145.567 kb on - strand, within MMP_RS05980 at 1145.568 kb on + strand, within MMP_RS05980 at 1145.568 kb on + strand, within MMP_RS05980 at 1145.635 kb on + strand, within MMP_RS05980 at 1145.648 kb on - strand, within MMP_RS05980 at 1145.676 kb on + strand, within MMP_RS05980 at 1145.688 kb on + strand, within MMP_RS05980 at 1145.745 kb on + strand, within MMP_RS05980 at 1145.765 kb on + strand, within MMP_RS05980 at 1145.765 kb on + strand, within MMP_RS05980 at 1145.765 kb on + strand, within MMP_RS05980 at 1145.843 kb on + strand at 1145.859 kb on + strand at 1145.961 kb on + strand, within MMP_RS05985 at 1145.961 kb on + strand, within MMP_RS05985 at 1145.961 kb on + strand, within MMP_RS05985 at 1146.039 kb on + strand at 1146.041 kb on + strand at 1146.052 kb on - strand at 1146.053 kb on + strand at 1146.073 kb on - strand at 1146.105 kb on - strand at 1146.292 kb on - strand, within MMP_RS05990 at 1146.312 kb on + strand, within MMP_RS05990 at 1146.335 kb on - strand, within MMP_RS05990 at 1146.350 kb on + strand, within MMP_RS05990 at 1146.377 kb on - strand, within MMP_RS05990 at 1146.432 kb on - strand at 1146.435 kb on - strand at 1146.435 kb on - strand at 1146.461 kb on - strand at 1146.462 kb on + strand at 1146.573 kb on + strand at 1146.578 kb on + strand at 1146.603 kb on - strand, within MMP_RS05995 at 1146.760 kb on + strand, within MMP_RS05995 at 1146.797 kb on - strand, within MMP_RS05995 at 1146.798 kb on + strand, within MMP_RS05995 at 1146.820 kb on - strand, within MMP_RS05995 at 1146.823 kb on + strand, within MMP_RS05995
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,144,544 + MMP_RS05965 0.69 +1.0 1,144,580 + MMP_RS05965 0.81 -0.5 1,144,580 + MMP_RS05965 0.81 +0.8 1,144,653 + -1.3 1,144,785 + MMP_RS05970 0.37 -0.5 1,144,829 - MMP_RS05970 0.49 -1.4 1,144,830 + MMP_RS05970 0.49 +1.1 1,144,853 - MMP_RS05970 0.56 +1.0 1,145,108 - MMP_RS05975 0.29 +1.6 1,145,288 - +0.9 1,145,292 + +0.2 1,145,303 + -0.6 1,145,319 - -0.4 1,145,319 - -1.4 1,145,320 + -1.8 1,145,357 - -0.5 1,145,357 - -2.0 1,145,357 - -3.4 1,145,358 + -0.2 1,145,358 + +0.1 1,145,362 + -0.3 1,145,380 - MMP_RS05980 0.12 +0.9 1,145,380 - MMP_RS05980 0.12 -1.1 1,145,447 - MMP_RS05980 0.25 +0.5 1,145,539 + MMP_RS05980 0.42 -1.0 1,145,567 - MMP_RS05980 0.48 -1.7 1,145,568 + MMP_RS05980 0.48 -1.7 1,145,568 + MMP_RS05980 0.48 +0.1 1,145,635 + MMP_RS05980 0.61 -0.4 1,145,648 - MMP_RS05980 0.64 -2.2 1,145,676 + MMP_RS05980 0.69 -1.5 1,145,688 + MMP_RS05980 0.71 +0.4 1,145,745 + MMP_RS05980 0.82 +0.2 1,145,765 + MMP_RS05980 0.86 +0.5 1,145,765 + MMP_RS05980 0.86 -0.1 1,145,765 + MMP_RS05980 0.86 -0.7 1,145,843 + -5.2 1,145,859 + +1.7 1,145,961 + MMP_RS05985 0.57 +0.7 1,145,961 + MMP_RS05985 0.57 -1.4 1,145,961 + MMP_RS05985 0.57 +1.3 1,146,039 + -0.8 1,146,041 + +0.3 1,146,052 - +0.3 1,146,053 + +0.7 1,146,073 - +1.3 1,146,105 - +0.2 1,146,292 - MMP_RS05990 0.61 -1.6 1,146,312 + MMP_RS05990 0.67 -0.1 1,146,335 - MMP_RS05990 0.73 +0.6 1,146,350 + MMP_RS05990 0.77 -0.9 1,146,377 - MMP_RS05990 0.85 +2.5 1,146,432 - +0.1 1,146,435 - -0.2 1,146,435 - -1.6 1,146,461 - +0.4 1,146,462 + +1.8 1,146,573 + -0.4 1,146,578 + -1.2 1,146,603 - MMP_RS05995 0.12 -0.7 1,146,760 + MMP_RS05995 0.25 +0.7 1,146,797 - MMP_RS05995 0.28 +0.2 1,146,798 + MMP_RS05995 0.28 -2.1 1,146,820 - MMP_RS05995 0.30 -0.6 1,146,823 + MMP_RS05995 0.31 +1.4
Or see this region's nucleotide sequence