Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05900 and MMP_RS05905 are separated by 27 nucleotides MMP_RS05905 and MMP_RS05910 are separated by 154 nucleotides MMP_RS05910 and MMP_RS05915 are separated by 50 nucleotides
MMP_RS05900: MMP_RS05900 - tetratricopeptide repeat protein, at 1,133,478 to 1,133,846
_RS05900
MMP_RS05905: MMP_RS05905 - undecaprenyl-diphosphate phosphatase, at 1,133,874 to 1,134,623
_RS05905
MMP_RS05910: MMP_RS05910 - cell division ATPase MinD, at 1,134,778 to 1,135,566
_RS05910
MMP_RS05915: MMP_RS05915 - amidophosphoribosyltransferase, at 1,135,617 to 1,136,996
_RS05915
Position (kb)
1134
1135
1136 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1133.890 kb on + strand at 1134.202 kb on + strand, within MMP_RS05905 at 1134.227 kb on - strand, within MMP_RS05905 at 1134.246 kb on + strand, within MMP_RS05905 at 1134.349 kb on + strand, within MMP_RS05905 at 1134.359 kb on - strand, within MMP_RS05905 at 1134.402 kb on - strand, within MMP_RS05905 at 1134.402 kb on - strand, within MMP_RS05905 at 1134.435 kb on - strand, within MMP_RS05905 at 1134.547 kb on + strand, within MMP_RS05905 at 1134.587 kb on - strand at 1134.643 kb on + strand at 1134.643 kb on + strand at 1134.664 kb on - strand at 1134.665 kb on + strand at 1134.667 kb on - strand at 1134.668 kb on + strand at 1134.679 kb on - strand at 1134.680 kb on + strand at 1134.702 kb on - strand at 1134.710 kb on - strand at 1134.730 kb on - strand at 1134.730 kb on - strand at 1134.731 kb on + strand at 1134.737 kb on - strand at 1135.181 kb on - strand, within MMP_RS05910 at 1135.322 kb on - strand, within MMP_RS05910 at 1135.444 kb on - strand, within MMP_RS05910 at 1135.461 kb on + strand, within MMP_RS05910 at 1135.592 kb on - strand at 1135.593 kb on + strand at 1135.801 kb on + strand at 1136.151 kb on - strand at 1136.318 kb on - strand at 1136.318 kb on - strand at 1136.487 kb on + strand at 1136.552 kb on - strand at 1136.553 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,133,890 + +0.9 1,134,202 + MMP_RS05905 0.44 -0.1 1,134,227 - MMP_RS05905 0.47 +0.6 1,134,246 + MMP_RS05905 0.50 -0.8 1,134,349 + MMP_RS05905 0.63 -1.6 1,134,359 - MMP_RS05905 0.65 -0.0 1,134,402 - MMP_RS05905 0.70 +0.4 1,134,402 - MMP_RS05905 0.70 +0.3 1,134,435 - MMP_RS05905 0.75 -1.1 1,134,547 + MMP_RS05905 0.90 +0.4 1,134,587 - -3.2 1,134,643 + -1.5 1,134,643 + +0.6 1,134,664 - -3.3 1,134,665 + +0.3 1,134,667 - -1.1 1,134,668 + +1.3 1,134,679 - +2.1 1,134,680 + +0.6 1,134,702 - +1.5 1,134,710 - -0.3 1,134,730 - -0.1 1,134,730 - -0.4 1,134,731 + -1.8 1,134,737 - -0.1 1,135,181 - MMP_RS05910 0.51 -2.0 1,135,322 - MMP_RS05910 0.69 -1.2 1,135,444 - MMP_RS05910 0.84 -2.2 1,135,461 + MMP_RS05910 0.87 +0.5 1,135,592 - +2.0 1,135,593 + -2.4 1,135,801 + -2.9 1,136,151 - -2.0 1,136,318 - +1.5 1,136,318 - -1.8 1,136,487 + -1.0 1,136,552 - -1.5 1,136,553 + -0.8
Or see this region's nucleotide sequence