Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05820 and MMP_RS05825 are separated by 31 nucleotides MMP_RS05825 and MMP_RS05830 are separated by 41 nucleotides MMP_RS05830 and MMP_RS05835 are separated by 114 nucleotides MMP_RS05835 and MMP_RS05840 are separated by 74 nucleotides
MMP_RS05820: MMP_RS05820 - RNA-binding transcriptional accessory protein, at 1,116,933 to 1,119,074
_RS05820
MMP_RS05825: MMP_RS05825 - peptidyl-prolyl cis-trans isomerase, at 1,119,106 to 1,119,717
_RS05825
MMP_RS05830: MMP_RS05830 - peptidyl-prolyl cis-trans isomerase, at 1,119,759 to 1,120,247
_RS05830
MMP_RS05835: MMP_RS05835 - secondary thiamine-phosphate synthase enzyme YjbQ, at 1,120,362 to 1,120,760
_RS05835
MMP_RS05840: MMP_RS05840 - peptide chain release factor aRF-1, at 1,120,835 to 1,122,094
_RS05840
Position (kb)
1119
1120
1121 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1118.903 kb on - strand at 1118.963 kb on - strand at 1118.964 kb on + strand at 1118.983 kb on - strand at 1118.983 kb on - strand at 1119.095 kb on - strand at 1119.099 kb on - strand at 1119.100 kb on + strand at 1119.145 kb on - strand at 1119.145 kb on - strand at 1119.285 kb on + strand, within MMP_RS05825 at 1119.539 kb on + strand, within MMP_RS05825 at 1119.608 kb on + strand, within MMP_RS05825 at 1119.649 kb on + strand, within MMP_RS05825 at 1119.786 kb on - strand at 1119.787 kb on + strand at 1119.941 kb on - strand, within MMP_RS05830 at 1119.973 kb on + strand, within MMP_RS05830 at 1120.111 kb on + strand, within MMP_RS05830 at 1120.113 kb on - strand, within MMP_RS05830 at 1120.247 kb on - strand at 1120.566 kb on - strand, within MMP_RS05835 at 1120.761 kb on + strand at 1120.822 kb on - strand at 1120.969 kb on - strand at 1120.969 kb on - strand at 1121.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,118,903 - +0.0 1,118,963 - +0.6 1,118,964 + -2.7 1,118,983 - -1.1 1,118,983 - -1.6 1,119,095 - -0.6 1,119,099 - -0.5 1,119,100 + +1.8 1,119,145 - +0.1 1,119,145 - +2.2 1,119,285 + MMP_RS05825 0.29 -2.8 1,119,539 + MMP_RS05825 0.71 -1.5 1,119,608 + MMP_RS05825 0.82 +0.3 1,119,649 + MMP_RS05825 0.89 +0.8 1,119,786 - -2.1 1,119,787 + -0.3 1,119,941 - MMP_RS05830 0.37 -0.0 1,119,973 + MMP_RS05830 0.44 +0.0 1,120,111 + MMP_RS05830 0.72 -0.6 1,120,113 - MMP_RS05830 0.72 -1.5 1,120,247 - -0.3 1,120,566 - MMP_RS05835 0.51 -0.5 1,120,761 + -2.1 1,120,822 - +1.5 1,120,969 - +1.2 1,120,969 - -1.2 1,121,134 - -0.2
Or see this region's nucleotide sequence