Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05655 and MMP_RS05660 are separated by 243 nucleotides MMP_RS05660 and MMP_RS05665 are separated by 81 nucleotides MMP_RS05665 and MMP_RS05670 are separated by 12 nucleotides
MMP_RS05655: MMP_RS05655 - phosphoenolpyruvate synthase, at 1,085,885 to 1,088,161
_RS05655
MMP_RS05660: MMP_RS05660 - phosphate ABC transporter substrate-binding protein, at 1,088,405 to 1,089,244
_RS05660
MMP_RS05665: MMP_RS05665 - phosphate ABC transporter permease subunit PstC, at 1,089,326 to 1,090,201
_RS05665
MMP_RS05670: MMP_RS05670 - phosphate ABC transporter permease PstA, at 1,090,214 to 1,091,080
_RS05670
Position (kb)
1088
1089
1090 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1087.469 kb on + strand at 1087.557 kb on + strand at 1087.557 kb on + strand at 1087.858 kb on + strand at 1087.918 kb on - strand at 1088.210 kb on - strand at 1088.243 kb on - strand at 1088.244 kb on + strand at 1088.245 kb on - strand at 1088.245 kb on - strand at 1088.246 kb on + strand at 1088.246 kb on + strand at 1088.247 kb on - strand at 1088.247 kb on - strand at 1088.248 kb on + strand at 1088.248 kb on + strand at 1088.283 kb on + strand at 1088.286 kb on + strand at 1088.418 kb on - strand at 1088.418 kb on - strand at 1088.419 kb on + strand at 1088.452 kb on + strand at 1088.492 kb on + strand, within MMP_RS05660 at 1088.516 kb on + strand, within MMP_RS05660 at 1088.536 kb on - strand, within MMP_RS05660 at 1088.598 kb on + strand, within MMP_RS05660 at 1088.758 kb on - strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.110 kb on - strand, within MMP_RS05660 at 1089.129 kb on - strand, within MMP_RS05660 at 1089.190 kb on + strand at 1089.216 kb on - strand at 1089.217 kb on + strand at 1089.229 kb on - strand at 1089.230 kb on + strand at 1089.243 kb on + strand at 1089.247 kb on - strand at 1089.248 kb on + strand at 1089.253 kb on + strand at 1089.313 kb on + strand at 1089.634 kb on - strand, within MMP_RS05665 at 1089.783 kb on - strand, within MMP_RS05665 at 1089.802 kb on - strand, within MMP_RS05665 at 1089.808 kb on + strand, within MMP_RS05665 at 1090.054 kb on - strand, within MMP_RS05665 at 1090.062 kb on - strand, within MMP_RS05665 at 1090.155 kb on - strand at 1090.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,087,469 + -0.5 1,087,557 + -1.0 1,087,557 + -0.3 1,087,858 + +0.5 1,087,918 - -1.1 1,088,210 - -1.1 1,088,243 - +0.5 1,088,244 + -0.2 1,088,245 - +0.4 1,088,245 - +1.4 1,088,246 + -1.0 1,088,246 + -0.8 1,088,247 - +0.4 1,088,247 - -0.0 1,088,248 + +0.0 1,088,248 + -0.5 1,088,283 + -2.6 1,088,286 + -1.7 1,088,418 - +1.1 1,088,418 - +0.7 1,088,419 + -1.9 1,088,452 + -0.5 1,088,492 + MMP_RS05660 0.10 +1.1 1,088,516 + MMP_RS05660 0.13 -0.6 1,088,536 - MMP_RS05660 0.16 -2.7 1,088,598 + MMP_RS05660 0.23 -0.3 1,088,758 - MMP_RS05660 0.42 -0.6 1,089,097 + MMP_RS05660 0.82 -4.5 1,089,097 + MMP_RS05660 0.82 -2.2 1,089,097 + MMP_RS05660 0.82 -2.9 1,089,110 - MMP_RS05660 0.84 +1.1 1,089,129 - MMP_RS05660 0.86 -0.8 1,089,190 + -0.5 1,089,216 - +1.6 1,089,217 + -0.2 1,089,229 - +0.4 1,089,230 + +0.4 1,089,243 + -0.9 1,089,247 - +1.3 1,089,248 + -1.2 1,089,253 + +0.1 1,089,313 + +2.4 1,089,634 - MMP_RS05665 0.35 -0.2 1,089,783 - MMP_RS05665 0.52 -1.8 1,089,802 - MMP_RS05665 0.54 -3.8 1,089,808 + MMP_RS05665 0.55 +0.4 1,090,054 - MMP_RS05665 0.83 +1.5 1,090,062 - MMP_RS05665 0.84 -0.6 1,090,155 - +1.3 1,090,167 + +0.3
Or see this region's nucleotide sequence