Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05645 and MMP_RS05650 overlap by 4 nucleotides MMP_RS05650 and MMP_RS05655 are separated by 70 nucleotides MMP_RS05655 and MMP_RS05660 are separated by 243 nucleotides
MMP_RS05645: MMP_RS05645 - AEC family transporter, at 1,084,457 to 1,085,371
_RS05645
MMP_RS05650: MMP_RS05650 - phosphopantetheine adenylyltransferase, at 1,085,368 to 1,085,814
_RS05650
MMP_RS05655: MMP_RS05655 - phosphoenolpyruvate synthase, at 1,085,885 to 1,088,161
_RS05655
MMP_RS05660: MMP_RS05660 - phosphate ABC transporter substrate-binding protein, at 1,088,405 to 1,089,244
_RS05660
Position (kb)
1085
1086
1087
1088
1089 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1084.941 kb on - strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.978 kb on - strand, within MMP_RS05645 at 1085.158 kb on - strand, within MMP_RS05645 at 1085.166 kb on + strand, within MMP_RS05645 at 1085.212 kb on - strand, within MMP_RS05645 at 1085.215 kb on - strand, within MMP_RS05645 at 1085.349 kb on - strand at 1085.349 kb on - strand at 1085.349 kb on - strand at 1085.350 kb on + strand at 1085.350 kb on + strand at 1085.362 kb on - strand at 1085.552 kb on + strand at 1085.626 kb on + strand at 1085.712 kb on - strand at 1085.782 kb on + strand at 1085.790 kb on - strand at 1086.350 kb on + strand at 1086.907 kb on - strand at 1087.211 kb on + strand at 1087.469 kb on + strand at 1087.557 kb on + strand at 1087.557 kb on + strand at 1087.858 kb on + strand at 1087.918 kb on - strand at 1088.210 kb on - strand at 1088.243 kb on - strand at 1088.244 kb on + strand at 1088.245 kb on - strand at 1088.245 kb on - strand at 1088.246 kb on + strand at 1088.246 kb on + strand at 1088.247 kb on - strand at 1088.247 kb on - strand at 1088.248 kb on + strand at 1088.248 kb on + strand at 1088.283 kb on + strand at 1088.286 kb on + strand at 1088.418 kb on - strand at 1088.418 kb on - strand at 1088.419 kb on + strand at 1088.452 kb on + strand at 1088.492 kb on + strand, within MMP_RS05660 at 1088.516 kb on + strand, within MMP_RS05660 at 1088.536 kb on - strand, within MMP_RS05660 at 1088.598 kb on + strand, within MMP_RS05660 at 1088.758 kb on - strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.097 kb on + strand, within MMP_RS05660 at 1089.110 kb on - strand, within MMP_RS05660 at 1089.129 kb on - strand, within MMP_RS05660
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 1,084,941 - MMP_RS05645 0.53 -1.4 1,084,942 + MMP_RS05645 0.53 +1.0 1,084,942 + MMP_RS05645 0.53 -0.3 1,084,942 + MMP_RS05645 0.53 +1.7 1,084,942 + MMP_RS05645 0.53 +0.6 1,084,978 - MMP_RS05645 0.57 +1.8 1,085,158 - MMP_RS05645 0.77 +0.8 1,085,166 + MMP_RS05645 0.77 -0.2 1,085,212 - MMP_RS05645 0.83 -1.1 1,085,215 - MMP_RS05645 0.83 -4.1 1,085,349 - +0.5 1,085,349 - +1.3 1,085,349 - +0.9 1,085,350 + +0.8 1,085,350 + +0.9 1,085,362 - -2.0 1,085,552 + -1.7 1,085,626 + -1.6 1,085,712 - +0.6 1,085,782 + -0.4 1,085,790 - -1.0 1,086,350 + +0.5 1,086,907 - -0.2 1,087,211 + -1.4 1,087,469 + -0.5 1,087,557 + -1.0 1,087,557 + -0.3 1,087,858 + +0.5 1,087,918 - -1.1 1,088,210 - -1.1 1,088,243 - +0.5 1,088,244 + -0.2 1,088,245 - +0.4 1,088,245 - +1.4 1,088,246 + -1.0 1,088,246 + -0.8 1,088,247 - +0.4 1,088,247 - -0.0 1,088,248 + +0.0 1,088,248 + -0.5 1,088,283 + -2.6 1,088,286 + -1.7 1,088,418 - +1.1 1,088,418 - +0.7 1,088,419 + -1.9 1,088,452 + -0.5 1,088,492 + MMP_RS05660 0.10 +1.1 1,088,516 + MMP_RS05660 0.13 -0.6 1,088,536 - MMP_RS05660 0.16 -2.7 1,088,598 + MMP_RS05660 0.23 -0.3 1,088,758 - MMP_RS05660 0.42 -0.6 1,089,097 + MMP_RS05660 0.82 -4.5 1,089,097 + MMP_RS05660 0.82 -2.2 1,089,097 + MMP_RS05660 0.82 -2.9 1,089,110 - MMP_RS05660 0.84 +1.1 1,089,129 - MMP_RS05660 0.86 -0.8
Or see this region's nucleotide sequence