Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04645 and MMP_RS04650 are separated by 123 nucleotides MMP_RS04650 and MMP_RS04655 are separated by 21 nucleotides
MMP_RS04645: MMP_RS04645 - glutamine-hydrolyzing GMP synthase, at 887,183 to 888,115
_RS04645
MMP_RS04650: MMP_RS04650 - tetratricopeptide repeat protein, at 888,239 to 888,832
_RS04650
MMP_RS04655: MMP_RS04655 - flippase, at 888,854 to 890,125
_RS04655
Position (kb) 888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 887.953 kb on - strand at 888.152 kb on + strand at 888.155 kb on - strand at 888.156 kb on + strand at 888.162 kb on - strand at 888.162 kb on - strand at 888.162 kb on - strand at 888.163 kb on + strand at 888.166 kb on - strand at 888.167 kb on + strand at 888.167 kb on + strand at 888.193 kb on - strand at 888.193 kb on - strand at 888.194 kb on + strand at 888.194 kb on + strand at 888.194 kb on + strand at 888.292 kb on - strand at 888.458 kb on - strand, within MMP_RS04650 at 888.472 kb on + strand, within MMP_RS04650 at 888.489 kb on - strand, within MMP_RS04650 at 888.524 kb on - strand, within MMP_RS04650 at 888.524 kb on - strand, within MMP_RS04650 at 888.526 kb on - strand, within MMP_RS04650 at 888.586 kb on - strand, within MMP_RS04650 at 888.612 kb on - strand, within MMP_RS04650 at 888.643 kb on - strand, within MMP_RS04650 at 888.653 kb on + strand, within MMP_RS04650 at 888.667 kb on + strand, within MMP_RS04650 at 888.684 kb on - strand, within MMP_RS04650 at 888.695 kb on + strand, within MMP_RS04650 at 888.713 kb on - strand, within MMP_RS04650 at 888.713 kb on - strand, within MMP_RS04650 at 888.714 kb on + strand, within MMP_RS04650 at 888.714 kb on + strand, within MMP_RS04650 at 888.714 kb on + strand, within MMP_RS04650 at 888.868 kb on + strand at 888.899 kb on - strand at 888.899 kb on - strand at 888.900 kb on + strand at 888.900 kb on + strand at 888.900 kb on + strand at 888.920 kb on - strand at 888.965 kb on + strand at 888.990 kb on - strand at 889.015 kb on + strand at 889.023 kb on - strand at 889.024 kb on + strand at 889.106 kb on - strand at 889.112 kb on + strand at 889.201 kb on + strand at 889.320 kb on - strand at 889.320 kb on - strand at 889.393 kb on - strand at 889.461 kb on + strand at 889.487 kb on + strand at 889.528 kb on - strand at 889.530 kb on - strand at 889.530 kb on - strand at 889.530 kb on - strand at 889.531 kb on + strand at 889.533 kb on + strand at 889.594 kb on - strand at 889.602 kb on - strand at 889.627 kb on - strand at 889.702 kb on + strand at 889.788 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 887,953 - -1.0 888,152 + +1.2 888,155 - -0.3 888,156 + +0.8 888,162 - +0.9 888,162 - +0.6 888,162 - -0.4 888,163 + +0.5 888,166 - -1.1 888,167 + -0.0 888,167 + +0.7 888,193 - -0.5 888,193 - -1.4 888,194 + +1.6 888,194 + -0.4 888,194 + +0.5 888,292 - +1.3 888,458 - MMP_RS04650 0.37 -0.5 888,472 + MMP_RS04650 0.39 +0.3 888,489 - MMP_RS04650 0.42 -1.9 888,524 - MMP_RS04650 0.48 -1.6 888,524 - MMP_RS04650 0.48 -0.6 888,526 - MMP_RS04650 0.48 +1.1 888,586 - MMP_RS04650 0.58 -0.1 888,612 - MMP_RS04650 0.63 -2.3 888,643 - MMP_RS04650 0.68 -1.0 888,653 + MMP_RS04650 0.70 -3.3 888,667 + MMP_RS04650 0.72 -3.7 888,684 - MMP_RS04650 0.75 +0.9 888,695 + MMP_RS04650 0.77 -1.5 888,713 - MMP_RS04650 0.80 +0.6 888,713 - MMP_RS04650 0.80 +0.8 888,714 + MMP_RS04650 0.80 +1.4 888,714 + MMP_RS04650 0.80 -0.6 888,714 + MMP_RS04650 0.80 -2.0 888,868 + -1.7 888,899 - +0.6 888,899 - +0.9 888,900 + -1.8 888,900 + -1.7 888,900 + -2.2 888,920 - -0.6 888,965 + -2.0 888,990 - -0.4 889,015 + -0.6 889,023 - -0.2 889,024 + -1.1 889,106 - -0.8 889,112 + -3.0 889,201 + -0.5 889,320 - +0.1 889,320 - -1.3 889,393 - -0.2 889,461 + -1.2 889,487 + -0.7 889,528 - +0.4 889,530 - -0.8 889,530 - -1.3 889,530 - +0.1 889,531 + -0.4 889,533 + +1.2 889,594 - -0.9 889,602 - -0.7 889,627 - -1.8 889,702 + -1.0 889,788 - +0.2
Or see this region's nucleotide sequence