Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04625 and MMP_RS04630 are separated by 213 nucleotides MMP_RS04630 and MMP_RS04635 are separated by 23 nucleotides MMP_RS04635 and MMP_RS04640 are separated by 163 nucleotides
MMP_RS04625: MMP_RS04625 - DEAD/DEAH box helicase, at 881,791 to 884,034
_RS04625
MMP_RS04630: MMP_RS04630 - 3'-5' exonuclease, at 884,248 to 884,823
_RS04630
MMP_RS04635: MMP_RS04635 - GNAT family N-acetyltransferase, at 884,847 to 885,395
_RS04635
MMP_RS04640: MMP_RS04640 - CTP synthase (glutamine hydrolyzing), at 885,559 to 887,160
_RS04640
Position (kb) 884
885
886 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 883.891 kb on - strand at 883.911 kb on - strand at 884.002 kb on - strand at 884.043 kb on - strand at 884.064 kb on + strand at 884.149 kb on + strand at 884.159 kb on - strand at 884.159 kb on - strand at 884.159 kb on - strand at 884.160 kb on + strand at 884.163 kb on - strand at 884.166 kb on + strand at 884.173 kb on + strand at 884.184 kb on - strand at 884.185 kb on + strand at 884.185 kb on + strand at 884.186 kb on - strand at 884.186 kb on - strand at 884.186 kb on - strand at 884.186 kb on - strand at 884.186 kb on - strand at 884.186 kb on - strand at 884.187 kb on + strand at 884.187 kb on + strand at 884.187 kb on + strand at 884.187 kb on + strand at 884.187 kb on + strand at 884.187 kb on + strand at 884.187 kb on + strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.188 kb on - strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.189 kb on + strand at 884.190 kb on - strand at 884.190 kb on - strand at 884.190 kb on - strand at 884.190 kb on - strand at 884.190 kb on - strand at 884.190 kb on - strand at 884.190 kb on - strand at 884.190 kb on - strand at 884.191 kb on + strand at 884.191 kb on + strand at 884.191 kb on + strand at 884.191 kb on + strand at 884.191 kb on + strand at 884.191 kb on + strand at 884.191 kb on + strand at 884.200 kb on + strand at 884.262 kb on + strand at 884.471 kb on - strand, within MMP_RS04630 at 884.496 kb on + strand, within MMP_RS04630 at 884.511 kb on - strand, within MMP_RS04630 at 884.525 kb on - strand, within MMP_RS04630 at 884.581 kb on + strand, within MMP_RS04630 at 884.581 kb on + strand, within MMP_RS04630 at 884.638 kb on - strand, within MMP_RS04630 at 884.641 kb on + strand, within MMP_RS04630 at 884.756 kb on + strand, within MMP_RS04630 at 884.769 kb on + strand at 884.771 kb on - strand at 884.771 kb on - strand at 884.771 kb on - strand at 884.773 kb on - strand at 884.774 kb on + strand at 884.810 kb on - strand at 884.811 kb on + strand at 884.837 kb on + strand at 885.129 kb on - strand, within MMP_RS04635 at 885.177 kb on + strand, within MMP_RS04635 at 885.177 kb on + strand, within MMP_RS04635 at 885.178 kb on - strand, within MMP_RS04635 at 885.183 kb on + strand, within MMP_RS04635 at 885.185 kb on - strand, within MMP_RS04635 at 885.191 kb on - strand, within MMP_RS04635 at 885.191 kb on - strand, within MMP_RS04635 at 885.248 kb on - strand, within MMP_RS04635 at 885.274 kb on + strand, within MMP_RS04635 at 885.351 kb on - strand at 885.352 kb on + strand at 885.352 kb on + strand at 885.360 kb on - strand at 885.378 kb on - strand at 885.378 kb on - strand at 885.378 kb on - strand at 885.384 kb on + strand at 885.395 kb on - strand at 885.396 kb on + strand at 885.396 kb on + strand at 885.416 kb on - strand at 885.417 kb on + strand at 885.425 kb on + strand at 885.426 kb on - strand at 885.426 kb on - strand at 885.427 kb on + strand at 885.427 kb on + strand at 885.427 kb on + strand at 885.439 kb on - strand at 885.439 kb on - strand at 885.440 kb on + strand at 885.440 kb on + strand at 885.440 kb on + strand at 885.440 kb on + strand at 885.452 kb on - strand at 885.452 kb on - strand at 885.512 kb on + strand at 885.512 kb on + strand at 885.540 kb on - strand at 885.566 kb on + strand at 885.568 kb on + strand at 885.569 kb on - strand at 885.570 kb on + strand at 885.588 kb on + strand at 886.089 kb on - strand at 886.170 kb on - strand at 886.182 kb on - strand at 886.349 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 883,891 - -1.6 883,911 - +0.7 884,002 - -0.2 884,043 - -0.2 884,064 + +0.6 884,149 + +0.1 884,159 - +1.6 884,159 - +2.0 884,159 - -1.9 884,160 + +0.2 884,163 - -0.0 884,166 + -0.6 884,173 + +1.6 884,184 - +0.1 884,185 + -2.7 884,185 + +0.1 884,186 - -0.6 884,186 - +1.0 884,186 - -2.0 884,186 - -0.1 884,186 - +1.0 884,186 - -1.0 884,187 + -2.2 884,187 + +0.4 884,187 + -1.0 884,187 + +1.5 884,187 + +1.3 884,187 + +1.4 884,187 + +2.0 884,188 - +1.4 884,188 - -0.9 884,188 - -2.6 884,188 - +1.1 884,188 - -0.6 884,189 + -1.0 884,189 + -0.0 884,189 + +0.8 884,189 + +0.1 884,189 + +0.7 884,189 + +0.5 884,190 - +0.6 884,190 - +1.0 884,190 - +0.8 884,190 - -0.4 884,190 - +1.5 884,190 - +0.7 884,190 - +0.9 884,190 - -0.8 884,191 + +0.3 884,191 + -1.1 884,191 + -2.3 884,191 + -0.3 884,191 + -0.7 884,191 + +1.5 884,191 + +0.9 884,200 + -0.8 884,262 + +1.3 884,471 - MMP_RS04630 0.39 +1.3 884,496 + MMP_RS04630 0.43 -2.5 884,511 - MMP_RS04630 0.46 -0.0 884,525 - MMP_RS04630 0.48 +0.8 884,581 + MMP_RS04630 0.58 -3.3 884,581 + MMP_RS04630 0.58 -2.5 884,638 - MMP_RS04630 0.68 -0.9 884,641 + MMP_RS04630 0.68 -0.4 884,756 + MMP_RS04630 0.88 -0.2 884,769 + -2.1 884,771 - -2.9 884,771 - +2.1 884,771 - -3.0 884,773 - +0.7 884,774 + +0.8 884,810 - -0.3 884,811 + +1.0 884,837 + -2.0 885,129 - MMP_RS04635 0.51 -0.8 885,177 + MMP_RS04635 0.60 -0.8 885,177 + MMP_RS04635 0.60 -0.7 885,178 - MMP_RS04635 0.60 +0.9 885,183 + MMP_RS04635 0.61 -1.1 885,185 - MMP_RS04635 0.62 -2.5 885,191 - MMP_RS04635 0.63 +1.4 885,191 - MMP_RS04635 0.63 -1.0 885,248 - MMP_RS04635 0.73 -0.8 885,274 + MMP_RS04635 0.78 -2.5 885,351 - +0.7 885,352 + +0.6 885,352 + -1.1 885,360 - +1.7 885,378 - -1.5 885,378 - +1.0 885,378 - -2.5 885,384 + +0.7 885,395 - +1.7 885,396 + +1.8 885,396 + -1.5 885,416 - +0.5 885,417 + +0.7 885,425 + -1.4 885,426 - -0.2 885,426 - +1.6 885,427 + +1.4 885,427 + +1.9 885,427 + +1.2 885,439 - -0.2 885,439 - +0.7 885,440 + -0.6 885,440 + -3.3 885,440 + +1.1 885,440 + -0.4 885,452 - -1.7 885,452 - -0.4 885,512 + +1.4 885,512 + -0.5 885,540 - -0.5 885,566 + -0.6 885,568 + +0.4 885,569 - +1.6 885,570 + +0.6 885,588 + -0.8 886,089 - -1.0 886,170 - +1.5 886,182 - -0.8 886,349 - +0.9
Or see this region's nucleotide sequence