Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03430 and MMP_RS03435 are separated by 119 nucleotides MMP_RS03435 and MMP_RS03440 are separated by 57 nucleotides MMP_RS03440 and MMP_RS03445 are separated by 75 nucleotides MMP_RS03445 and MMP_RS03450 are separated by 74 nucleotides
MMP_RS03430: MMP_RS03430 - bile acid:sodium symporter family protein, at 644,644 to 645,609
_RS03430
MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721
_RS03435
MMP_RS03440: MMP_RS03440 - MoaD/ThiS family protein, at 646,779 to 646,973
_RS03440
MMP_RS03445: MMP_RS03445 - methanogenesis marker 17 protein, at 647,049 to 647,600
_RS03445
MMP_RS03450: MMP_RS03450 - glycine synthesis protein GlyXS <small>(from data)</small>, at 647,675 to 647,947
_RS03450
Position (kb) 645
646
647 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 644.864 kb on - strand, within MMP_RS03430 at 644.865 kb on + strand, within MMP_RS03430 at 644.895 kb on - strand, within MMP_RS03430 at 644.895 kb on - strand, within MMP_RS03430 at 644.971 kb on - strand, within MMP_RS03430 at 645.014 kb on + strand, within MMP_RS03430 at 645.042 kb on - strand, within MMP_RS03430 at 645.048 kb on - strand, within MMP_RS03430 at 645.107 kb on - strand, within MMP_RS03430 at 645.146 kb on + strand, within MMP_RS03430 at 645.230 kb on - strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.314 kb on + strand, within MMP_RS03430 at 645.319 kb on + strand, within MMP_RS03430 at 645.417 kb on - strand, within MMP_RS03430 at 645.491 kb on + strand, within MMP_RS03430 at 645.683 kb on + strand at 645.700 kb on + strand at 645.737 kb on + strand at 645.755 kb on - strand at 645.917 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435 at 646.615 kb on - strand, within MMP_RS03435 at 646.660 kb on - strand at 646.720 kb on + strand at 646.870 kb on - strand, within MMP_RS03440 at 646.979 kb on - strand at 646.983 kb on - strand at 646.984 kb on + strand at 646.987 kb on - strand at 646.997 kb on + strand at 647.001 kb on - strand at 647.002 kb on + strand at 647.002 kb on + strand at 647.004 kb on + strand at 647.018 kb on - strand at 647.055 kb on - strand at 647.407 kb on - strand, within MMP_RS03445 at 647.695 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 644,864 - MMP_RS03430 0.23 +1.5 644,865 + MMP_RS03430 0.23 -1.0 644,895 - MMP_RS03430 0.26 +0.5 644,895 - MMP_RS03430 0.26 -1.1 644,971 - MMP_RS03430 0.34 -0.5 645,014 + MMP_RS03430 0.38 +0.7 645,042 - MMP_RS03430 0.41 -0.5 645,048 - MMP_RS03430 0.42 -0.4 645,107 - MMP_RS03430 0.48 -1.4 645,146 + MMP_RS03430 0.52 -1.2 645,230 - MMP_RS03430 0.61 +0.2 645,231 + MMP_RS03430 0.61 -1.8 645,231 + MMP_RS03430 0.61 +0.8 645,231 + MMP_RS03430 0.61 +1.3 645,314 + MMP_RS03430 0.69 +0.6 645,319 + MMP_RS03430 0.70 +2.3 645,417 - MMP_RS03430 0.80 +1.1 645,491 + MMP_RS03430 0.88 -0.9 645,683 + +1.3 645,700 + -1.3 645,737 + +0.5 645,755 - -2.0 645,917 + MMP_RS03435 0.19 -0.8 645,965 + MMP_RS03435 0.24 -0.8 645,965 + MMP_RS03435 0.24 +1.0 646,615 - MMP_RS03435 0.89 -0.8 646,660 - -1.6 646,720 + -3.8 646,870 - MMP_RS03440 0.47 +1.0 646,979 - -3.2 646,983 - +1.0 646,984 + -2.9 646,987 - -0.2 646,997 + -0.4 647,001 - -3.2 647,002 + +1.7 647,002 + -1.1 647,004 + -1.1 647,018 - -1.9 647,055 - -2.9 647,407 - MMP_RS03445 0.65 +1.0 647,695 - +1.5
Or see this region's nucleotide sequence