Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03420 and MMP_RS03425 are separated by 506 nucleotides MMP_RS03425 and MMP_RS03430 are separated by 21 nucleotides MMP_RS03430 and MMP_RS03435 are separated by 119 nucleotides
MMP_RS03420: MMP_RS03420 - acetolactate synthase small subunit, at 643,154 to 643,663
_RS03420
MMP_RS03425: MMP_RS03425 - hypothetical protein, at 644,170 to 644,622
_RS03425
MMP_RS03430: MMP_RS03430 - bile acid:sodium symporter family protein, at 644,644 to 645,609
_RS03430
MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721
_RS03435
Position (kb) 644
645
646 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 643.655 kb on + strand at 643.765 kb on + strand at 643.903 kb on + strand at 643.972 kb on - strand at 643.987 kb on - strand at 643.988 kb on + strand at 644.071 kb on + strand at 644.090 kb on + strand at 644.090 kb on + strand at 644.129 kb on - strand at 644.145 kb on + strand at 644.147 kb on + strand at 644.262 kb on - strand, within MMP_RS03425 at 644.268 kb on - strand, within MMP_RS03425 at 644.307 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.354 kb on + strand, within MMP_RS03425 at 644.419 kb on - strand, within MMP_RS03425 at 644.431 kb on + strand, within MMP_RS03425 at 644.485 kb on + strand, within MMP_RS03425 at 644.495 kb on + strand, within MMP_RS03425 at 644.562 kb on - strand, within MMP_RS03425 at 644.569 kb on - strand, within MMP_RS03425 at 644.621 kb on + strand at 644.864 kb on - strand, within MMP_RS03430 at 644.865 kb on + strand, within MMP_RS03430 at 644.895 kb on - strand, within MMP_RS03430 at 644.895 kb on - strand, within MMP_RS03430 at 644.971 kb on - strand, within MMP_RS03430 at 645.014 kb on + strand, within MMP_RS03430 at 645.042 kb on - strand, within MMP_RS03430 at 645.048 kb on - strand, within MMP_RS03430 at 645.107 kb on - strand, within MMP_RS03430 at 645.146 kb on + strand, within MMP_RS03430 at 645.230 kb on - strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.314 kb on + strand, within MMP_RS03430 at 645.319 kb on + strand, within MMP_RS03430 at 645.417 kb on - strand, within MMP_RS03430 at 645.491 kb on + strand, within MMP_RS03430 at 645.683 kb on + strand at 645.700 kb on + strand at 645.737 kb on + strand at 645.755 kb on - strand at 645.917 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 643,655 + -1.9 643,765 + -3.2 643,903 + +0.6 643,972 - +0.7 643,987 - -1.0 643,988 + -0.6 644,071 + +0.8 644,090 + -1.9 644,090 + +1.5 644,129 - +1.3 644,145 + -0.5 644,147 + -1.3 644,262 - MMP_RS03425 0.20 +0.3 644,268 - MMP_RS03425 0.22 -0.1 644,307 - MMP_RS03425 0.30 +1.1 644,324 - MMP_RS03425 0.34 +1.9 644,324 - MMP_RS03425 0.34 +1.7 644,354 + MMP_RS03425 0.41 +0.4 644,419 - MMP_RS03425 0.55 +1.3 644,431 + MMP_RS03425 0.58 +0.7 644,485 + MMP_RS03425 0.70 +0.8 644,495 + MMP_RS03425 0.72 +1.0 644,562 - MMP_RS03425 0.87 +0.6 644,569 - MMP_RS03425 0.88 -1.8 644,621 + -4.5 644,864 - MMP_RS03430 0.23 +1.5 644,865 + MMP_RS03430 0.23 -1.0 644,895 - MMP_RS03430 0.26 +0.5 644,895 - MMP_RS03430 0.26 -1.1 644,971 - MMP_RS03430 0.34 -0.5 645,014 + MMP_RS03430 0.38 +0.7 645,042 - MMP_RS03430 0.41 -0.5 645,048 - MMP_RS03430 0.42 -0.4 645,107 - MMP_RS03430 0.48 -1.4 645,146 + MMP_RS03430 0.52 -1.2 645,230 - MMP_RS03430 0.61 +0.2 645,231 + MMP_RS03430 0.61 -1.8 645,231 + MMP_RS03430 0.61 +0.8 645,231 + MMP_RS03430 0.61 +1.3 645,314 + MMP_RS03430 0.69 +0.6 645,319 + MMP_RS03430 0.70 +2.3 645,417 - MMP_RS03430 0.80 +1.1 645,491 + MMP_RS03430 0.88 -0.9 645,683 + +1.3 645,700 + -1.3 645,737 + +0.5 645,755 - -2.0 645,917 + MMP_RS03435 0.19 -0.8 645,965 + MMP_RS03435 0.24 -0.8 645,965 + MMP_RS03435 0.24 +1.0
Or see this region's nucleotide sequence