Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03315 and MMP_RS03320 are separated by 111 nucleotides MMP_RS03320 and MMP_RS03325 are separated by 180 nucleotides MMP_RS03325 and MMP_RS03330 are separated by 131 nucleotides MMP_RS03330 and MMP_RS03335 are separated by 153 nucleotides
MMP_RS03315: MMP_RS03315 - ferrous iron transport protein A, at 623,532 to 623,738
_RS03315
MMP_RS03320: MMP_RS03320 - metal-dependent transcriptional regulator, at 623,850 to 624,254
_RS03320
MMP_RS03325: MMP_RS03325 - hypothetical protein, at 624,435 to 625,601
_RS03325
MMP_RS03330: MMP_RS03330 - rubrerythrin family protein, at 625,733 to 626,323
_RS03330
MMP_RS03335: MMP_RS03335 - ABC transporter permease, at 626,477 to 627,670
_RS03335
Position (kb)
624
625
626 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 623.462 kb on - strand at 623.473 kb on + strand at 623.497 kb on + strand at 623.504 kb on + strand at 623.517 kb on - strand at 623.693 kb on - strand, within MMP_RS03315 at 623.702 kb on - strand, within MMP_RS03315 at 623.704 kb on - strand, within MMP_RS03315 at 623.755 kb on - strand at 623.755 kb on - strand at 623.756 kb on + strand at 623.822 kb on - strand at 623.823 kb on + strand at 623.823 kb on + strand at 623.824 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.826 kb on - strand at 623.827 kb on + strand at 623.828 kb on - strand at 623.828 kb on - strand at 623.828 kb on - strand at 623.887 kb on - strand at 623.897 kb on - strand, within MMP_RS03320 at 623.901 kb on + strand, within MMP_RS03320 at 623.909 kb on - strand, within MMP_RS03320 at 624.012 kb on - strand, within MMP_RS03320 at 624.023 kb on - strand, within MMP_RS03320 at 624.287 kb on - strand at 624.288 kb on + strand at 624.318 kb on + strand at 624.365 kb on - strand at 624.365 kb on - strand at 624.365 kb on - strand at 624.365 kb on - strand at 624.366 kb on + strand at 624.367 kb on - strand at 624.368 kb on + strand at 624.376 kb on - strand at 624.377 kb on + strand at 624.377 kb on + strand at 624.377 kb on + strand at 624.381 kb on + strand at 624.400 kb on - strand at 624.401 kb on + strand at 624.401 kb on + strand at 624.411 kb on - strand at 624.414 kb on - strand at 624.415 kb on + strand at 624.434 kb on - strand at 624.462 kb on + strand at 624.535 kb on - strand at 624.593 kb on + strand, within MMP_RS03325 at 624.593 kb on + strand, within MMP_RS03325 at 624.775 kb on + strand, within MMP_RS03325 at 624.777 kb on + strand, within MMP_RS03325 at 624.915 kb on + strand, within MMP_RS03325 at 624.990 kb on + strand, within MMP_RS03325 at 625.075 kb on - strand, within MMP_RS03325 at 625.099 kb on - strand, within MMP_RS03325 at 625.432 kb on - strand, within MMP_RS03325 at 625.496 kb on + strand at 625.574 kb on - strand at 625.645 kb on - strand at 625.668 kb on - strand at 625.669 kb on + strand at 625.669 kb on + strand at 625.689 kb on - strand at 625.697 kb on + strand at 625.795 kb on - strand, within MMP_RS03330 at 625.801 kb on - strand, within MMP_RS03330 at 625.801 kb on - strand, within MMP_RS03330 at 625.802 kb on + strand, within MMP_RS03330 at 625.802 kb on + strand, within MMP_RS03330 at 625.808 kb on + strand, within MMP_RS03330 at 625.808 kb on + strand, within MMP_RS03330 at 625.808 kb on + strand, within MMP_RS03330 at 625.812 kb on - strand, within MMP_RS03330 at 625.812 kb on - strand, within MMP_RS03330 at 625.812 kb on - strand, within MMP_RS03330 at 625.812 kb on - strand, within MMP_RS03330 at 625.812 kb on - strand, within MMP_RS03330 at 625.812 kb on - strand, within MMP_RS03330 at 625.813 kb on + strand, within MMP_RS03330 at 625.813 kb on + strand, within MMP_RS03330 at 625.813 kb on + strand, within MMP_RS03330 at 625.813 kb on + strand, within MMP_RS03330 at 625.815 kb on + strand, within MMP_RS03330 at 625.815 kb on + strand, within MMP_RS03330 at 625.860 kb on - strand, within MMP_RS03330 at 625.909 kb on - strand, within MMP_RS03330 at 625.915 kb on + strand, within MMP_RS03330 at 625.986 kb on + strand, within MMP_RS03330 at 625.993 kb on - strand, within MMP_RS03330 at 625.993 kb on - strand, within MMP_RS03330 at 625.994 kb on + strand, within MMP_RS03330 at 626.088 kb on - strand, within MMP_RS03330 at 626.110 kb on + strand, within MMP_RS03330 at 626.208 kb on - strand, within MMP_RS03330 at 626.209 kb on + strand, within MMP_RS03330 at 626.242 kb on + strand, within MMP_RS03330 at 626.294 kb on - strand at 626.319 kb on + strand at 626.375 kb on + strand at 626.396 kb on + strand at 626.551 kb on + strand at 626.563 kb on - strand at 626.581 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 623,462 - +0.8 623,473 + -0.7 623,497 + -0.9 623,504 + +0.2 623,517 - +0.9 623,693 - MMP_RS03315 0.78 -0.8 623,702 - MMP_RS03315 0.82 -0.6 623,704 - MMP_RS03315 0.83 -1.3 623,755 - +0.1 623,755 - -0.2 623,756 + -0.6 623,822 - -1.2 623,823 + -1.1 623,823 + -1.6 623,824 - -3.5 623,826 - -0.2 623,826 - +1.6 623,826 - +0.6 623,826 - -1.3 623,826 - -3.3 623,826 - -0.0 623,827 + -0.4 623,828 - +0.9 623,828 - +1.6 623,828 - -0.5 623,887 - -0.0 623,897 - MMP_RS03320 0.12 +1.0 623,901 + MMP_RS03320 0.13 +0.0 623,909 - MMP_RS03320 0.15 -0.4 624,012 - MMP_RS03320 0.40 +0.2 624,023 - MMP_RS03320 0.43 -0.2 624,287 - -0.2 624,288 + +0.3 624,318 + +0.9 624,365 - -1.0 624,365 - +0.8 624,365 - +1.0 624,365 - +0.7 624,366 + -2.7 624,367 - -0.6 624,368 + +0.3 624,376 - -1.1 624,377 + -2.1 624,377 + +0.4 624,377 + +1.3 624,381 + -1.5 624,400 - +0.8 624,401 + +1.2 624,401 + +0.8 624,411 - +0.9 624,414 - +1.1 624,415 + +1.6 624,434 - -2.5 624,462 + -0.7 624,535 - -0.8 624,593 + MMP_RS03325 0.14 +0.0 624,593 + MMP_RS03325 0.14 +0.5 624,775 + MMP_RS03325 0.29 -0.2 624,777 + MMP_RS03325 0.29 -1.2 624,915 + MMP_RS03325 0.41 -3.4 624,990 + MMP_RS03325 0.48 -0.8 625,075 - MMP_RS03325 0.55 +0.3 625,099 - MMP_RS03325 0.57 +0.9 625,432 - MMP_RS03325 0.85 +0.2 625,496 + -1.1 625,574 - -2.7 625,645 - +1.3 625,668 - +2.2 625,669 + +0.7 625,669 + -1.0 625,689 - -3.5 625,697 + -2.7 625,795 - MMP_RS03330 0.10 -0.7 625,801 - MMP_RS03330 0.12 +0.2 625,801 - MMP_RS03330 0.12 -3.0 625,802 + MMP_RS03330 0.12 +2.0 625,802 + MMP_RS03330 0.12 -0.3 625,808 + MMP_RS03330 0.13 +0.1 625,808 + MMP_RS03330 0.13 +1.5 625,808 + MMP_RS03330 0.13 -3.5 625,812 - MMP_RS03330 0.13 +0.5 625,812 - MMP_RS03330 0.13 +1.0 625,812 - MMP_RS03330 0.13 -0.8 625,812 - MMP_RS03330 0.13 -2.3 625,812 - MMP_RS03330 0.13 -1.8 625,812 - MMP_RS03330 0.13 +1.9 625,813 + MMP_RS03330 0.14 +1.5 625,813 + MMP_RS03330 0.14 +0.7 625,813 + MMP_RS03330 0.14 -0.6 625,813 + MMP_RS03330 0.14 +0.4 625,815 + MMP_RS03330 0.14 -1.6 625,815 + MMP_RS03330 0.14 -2.1 625,860 - MMP_RS03330 0.21 -0.7 625,909 - MMP_RS03330 0.30 -0.2 625,915 + MMP_RS03330 0.31 +2.0 625,986 + MMP_RS03330 0.43 -1.4 625,993 - MMP_RS03330 0.44 +0.6 625,993 - MMP_RS03330 0.44 -0.9 625,994 + MMP_RS03330 0.44 +0.7 626,088 - MMP_RS03330 0.60 +0.6 626,110 + MMP_RS03330 0.64 -1.6 626,208 - MMP_RS03330 0.80 +0.6 626,209 + MMP_RS03330 0.81 +0.2 626,242 + MMP_RS03330 0.86 +1.8 626,294 - +0.1 626,319 + -4.1 626,375 + +0.0 626,396 + +0.5 626,551 + -2.6 626,563 - -0.6 626,581 + -0.6
Or see this region's nucleotide sequence