Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02795 and MMP_RS02800 are separated by 230 nucleotides MMP_RS02800 and MMP_RS02805 overlap by 14 nucleotides MMP_RS02805 and MMP_RS02810 are separated by 157 nucleotides
MMP_RS02795: MMP_RS02795 - sulfate permease, at 533,633 to 535,291
_RS02795
MMP_RS02800: MMP_RS02800 - nucleotidyltransferase domain-containing protein, at 535,522 to 536,139
_RS02800
MMP_RS02805: MMP_RS02805 - HEPN domain-containing protein, at 536,126 to 536,635
_RS02805
MMP_RS02810: MMP_RS02810 - hypothetical protein, at 536,793 to 538,262
_RS02810
Position (kb) 535
536
537 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 534.572 kb on - strand, within MMP_RS02795 at 534.754 kb on - strand, within MMP_RS02795 at 534.755 kb on + strand, within MMP_RS02795 at 534.760 kb on - strand, within MMP_RS02795 at 534.760 kb on - strand, within MMP_RS02795 at 534.817 kb on - strand, within MMP_RS02795 at 534.822 kb on - strand, within MMP_RS02795 at 534.822 kb on - strand, within MMP_RS02795 at 534.822 kb on - strand, within MMP_RS02795 at 534.822 kb on - strand, within MMP_RS02795 at 534.827 kb on + strand, within MMP_RS02795 at 534.996 kb on + strand, within MMP_RS02795 at 535.000 kb on - strand, within MMP_RS02795 at 535.244 kb on - strand at 535.272 kb on + strand at 535.292 kb on + strand at 535.293 kb on - strand at 535.294 kb on + strand at 535.294 kb on + strand at 535.330 kb on - strand at 535.356 kb on - strand at 535.377 kb on - strand at 535.379 kb on - strand at 535.381 kb on - strand at 535.381 kb on - strand at 535.382 kb on + strand at 535.382 kb on + strand at 535.382 kb on + strand at 535.382 kb on + strand at 535.385 kb on - strand at 535.421 kb on - strand at 535.422 kb on + strand at 535.424 kb on + strand at 535.424 kb on + strand at 535.427 kb on - strand at 535.428 kb on + strand at 535.428 kb on + strand at 535.436 kb on + strand at 535.496 kb on - strand at 535.496 kb on - strand at 535.496 kb on - strand at 535.496 kb on - strand at 535.497 kb on + strand at 535.497 kb on + strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.498 kb on - strand at 535.499 kb on + strand at 535.499 kb on + strand at 535.499 kb on + strand at 535.499 kb on + strand at 535.502 kb on - strand at 535.509 kb on + strand at 535.541 kb on - strand at 535.541 kb on - strand at 535.542 kb on + strand at 535.542 kb on + strand at 535.543 kb on - strand at 535.552 kb on - strand at 535.553 kb on + strand at 535.572 kb on - strand at 535.577 kb on - strand at 535.693 kb on - strand, within MMP_RS02800 at 535.693 kb on - strand, within MMP_RS02800 at 535.696 kb on - strand, within MMP_RS02800 at 535.696 kb on - strand, within MMP_RS02800 at 535.697 kb on + strand, within MMP_RS02800 at 535.697 kb on + strand, within MMP_RS02800 at 535.697 kb on + strand, within MMP_RS02800 at 535.998 kb on + strand, within MMP_RS02800 at 536.013 kb on + strand, within MMP_RS02800 at 536.060 kb on - strand, within MMP_RS02800 at 536.144 kb on - strand at 536.318 kb on + strand, within MMP_RS02805 at 536.714 kb on - strand at 536.742 kb on - strand at 536.761 kb on - strand at 536.761 kb on - strand at 536.761 kb on - strand at 536.773 kb on + strand at 536.848 kb on - strand at 536.850 kb on - strand at 536.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 534,572 - MMP_RS02795 0.57 +0.0 534,754 - MMP_RS02795 0.68 -0.8 534,755 + MMP_RS02795 0.68 +0.7 534,760 - MMP_RS02795 0.68 -0.7 534,760 - MMP_RS02795 0.68 -1.3 534,817 - MMP_RS02795 0.71 -0.1 534,822 - MMP_RS02795 0.72 -3.6 534,822 - MMP_RS02795 0.72 +1.9 534,822 - MMP_RS02795 0.72 -1.3 534,822 - MMP_RS02795 0.72 -2.6 534,827 + MMP_RS02795 0.72 -3.6 534,996 + MMP_RS02795 0.82 -1.5 535,000 - MMP_RS02795 0.82 +0.2 535,244 - +0.9 535,272 + +0.4 535,292 + -1.1 535,293 - -1.1 535,294 + +0.3 535,294 + -3.0 535,330 - +1.9 535,356 - +0.9 535,377 - +1.2 535,379 - -2.1 535,381 - +0.0 535,381 - -0.4 535,382 + -1.4 535,382 + -2.6 535,382 + -1.5 535,382 + -0.0 535,385 - -0.3 535,421 - -0.3 535,422 + -3.0 535,424 + -2.3 535,424 + -1.1 535,427 - +2.3 535,428 + -2.3 535,428 + -0.9 535,436 + +0.1 535,496 - +0.5 535,496 - -4.6 535,496 - +0.7 535,496 - -3.0 535,497 + +0.5 535,497 + +0.8 535,498 - -1.4 535,498 - -1.9 535,498 - -3.2 535,498 - -1.1 535,498 - -0.3 535,498 - -1.5 535,498 - -2.2 535,499 + -4.4 535,499 + -3.1 535,499 + -2.8 535,499 + -2.0 535,502 - +0.4 535,509 + -0.7 535,541 - -0.8 535,541 - +0.9 535,542 + -0.4 535,542 + -1.2 535,543 - -1.6 535,552 - -4.3 535,553 + -0.5 535,572 - -0.3 535,577 - -0.8 535,693 - MMP_RS02800 0.28 -2.9 535,693 - MMP_RS02800 0.28 -0.2 535,696 - MMP_RS02800 0.28 -1.8 535,696 - MMP_RS02800 0.28 +0.3 535,697 + MMP_RS02800 0.28 -0.6 535,697 + MMP_RS02800 0.28 -1.2 535,697 + MMP_RS02800 0.28 -3.7 535,998 + MMP_RS02800 0.77 -1.7 536,013 + MMP_RS02800 0.79 -0.3 536,060 - MMP_RS02800 0.87 +1.8 536,144 - -1.2 536,318 + MMP_RS02805 0.38 -0.2 536,714 - +0.5 536,742 - -0.8 536,761 - +0.1 536,761 - +1.1 536,761 - +0.5 536,773 + +1.5 536,848 - +0.1 536,850 - +1.6 536,851 + +0.3
Or see this region's nucleotide sequence