Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02545 and MMP_RS02550 are separated by 249 nucleotides MMP_RS02550 and MMP_RS02555 are separated by 1 nucleotides MMP_RS02555 and MMP_RS02560 are separated by 367 nucleotides MMP_RS02560 and MMP_RS02565 overlap by 8 nucleotides
MMP_RS02545: MMP_RS02545 - hypothetical protein, at 475,377 to 475,787
_RS02545
MMP_RS02550: MMP_RS02550 - helix-turn-helix transcriptional regulator, at 476,037 to 476,249
_RS02550
MMP_RS02555: MMP_RS02555 - AIPR family protein, at 476,251 to 478,203
_RS02555
MMP_RS02560: MMP_RS02560 - DUF2283 domain-containing protein, at 478,571 to 478,954
_RS02560
MMP_RS02565: MMP_RS02565 - hypothetical protein, at 478,947 to 479,240
_RS02565
Position (kb) 476
477
478
479 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 475.271 kb on - strand at 475.350 kb on - strand at 475.413 kb on + strand at 475.414 kb on - strand at 475.415 kb on + strand at 475.459 kb on + strand, within MMP_RS02545 at 475.480 kb on + strand, within MMP_RS02545 at 475.504 kb on + strand, within MMP_RS02545 at 475.548 kb on - strand, within MMP_RS02545 at 475.605 kb on + strand, within MMP_RS02545 at 475.710 kb on - strand, within MMP_RS02545 at 475.724 kb on + strand, within MMP_RS02545 at 475.800 kb on - strand at 475.847 kb on - strand at 475.847 kb on - strand at 475.847 kb on - strand at 475.848 kb on + strand at 475.848 kb on + strand at 475.848 kb on + strand at 475.849 kb on - strand at 475.849 kb on - strand at 475.850 kb on + strand at 475.853 kb on - strand at 475.853 kb on - strand at 475.853 kb on - strand at 475.854 kb on + strand at 475.854 kb on + strand at 475.855 kb on - strand at 475.856 kb on + strand at 475.917 kb on - strand at 475.917 kb on - strand at 475.928 kb on + strand at 475.937 kb on - strand at 475.938 kb on + strand at 475.942 kb on + strand at 475.951 kb on + strand at 476.012 kb on + strand at 476.040 kb on - strand at 476.099 kb on + strand, within MMP_RS02550 at 476.116 kb on + strand, within MMP_RS02550 at 476.127 kb on - strand, within MMP_RS02550 at 476.129 kb on - strand, within MMP_RS02550 at 476.170 kb on + strand, within MMP_RS02550 at 476.170 kb on + strand, within MMP_RS02550 at 476.179 kb on - strand, within MMP_RS02550 at 476.253 kb on + strand at 476.255 kb on + strand at 476.290 kb on + strand at 476.314 kb on + strand at 476.337 kb on - strand at 476.377 kb on - strand at 476.408 kb on - strand at 476.409 kb on + strand at 476.445 kb on - strand at 476.446 kb on + strand at 476.453 kb on + strand, within MMP_RS02555 at 476.477 kb on - strand, within MMP_RS02555 at 476.478 kb on + strand, within MMP_RS02555 at 476.478 kb on + strand, within MMP_RS02555 at 476.478 kb on + strand, within MMP_RS02555 at 476.479 kb on - strand, within MMP_RS02555 at 476.510 kb on + strand, within MMP_RS02555 at 476.529 kb on - strand, within MMP_RS02555 at 476.562 kb on + strand, within MMP_RS02555 at 476.620 kb on + strand, within MMP_RS02555 at 476.648 kb on + strand, within MMP_RS02555 at 476.652 kb on - strand, within MMP_RS02555 at 476.685 kb on - strand, within MMP_RS02555 at 476.711 kb on + strand, within MMP_RS02555 at 476.714 kb on + strand, within MMP_RS02555 at 476.773 kb on - strand, within MMP_RS02555 at 476.799 kb on - strand, within MMP_RS02555 at 476.888 kb on - strand, within MMP_RS02555 at 476.894 kb on - strand, within MMP_RS02555 at 476.894 kb on - strand, within MMP_RS02555 at 476.895 kb on + strand, within MMP_RS02555 at 476.897 kb on + strand at 476.897 kb on + strand, within MMP_RS02555 at 476.929 kb on + strand, within MMP_RS02555 at 476.931 kb on - strand, within MMP_RS02555 at 476.946 kb on - strand, within MMP_RS02555 at 476.947 kb on + strand, within MMP_RS02555 at 476.960 kb on + strand, within MMP_RS02555 at 476.983 kb on + strand, within MMP_RS02555 at 477.048 kb on - strand, within MMP_RS02555 at 477.106 kb on + strand, within MMP_RS02555 at 477.106 kb on + strand, within MMP_RS02555 at 477.106 kb on + strand, within MMP_RS02555 at 477.110 kb on - strand, within MMP_RS02555 at 477.111 kb on + strand, within MMP_RS02555 at 477.111 kb on + strand, within MMP_RS02555 at 477.118 kb on + strand, within MMP_RS02555 at 477.153 kb on - strand, within MMP_RS02555 at 477.160 kb on + strand, within MMP_RS02555 at 477.166 kb on - strand, within MMP_RS02555 at 477.180 kb on - strand, within MMP_RS02555 at 477.184 kb on - strand, within MMP_RS02555 at 477.205 kb on + strand, within MMP_RS02555 at 477.218 kb on - strand, within MMP_RS02555 at 477.227 kb on - strand, within MMP_RS02555 at 477.228 kb on + strand, within MMP_RS02555 at 477.228 kb on + strand, within MMP_RS02555 at 477.274 kb on + strand, within MMP_RS02555 at 477.297 kb on - strand, within MMP_RS02555 at 477.300 kb on - strand, within MMP_RS02555 at 477.300 kb on - strand, within MMP_RS02555 at 477.400 kb on + strand, within MMP_RS02555 at 477.400 kb on + strand, within MMP_RS02555 at 477.427 kb on - strand, within MMP_RS02555 at 477.428 kb on + strand, within MMP_RS02555 at 477.475 kb on - strand, within MMP_RS02555 at 477.496 kb on + strand, within MMP_RS02555 at 477.503 kb on - strand, within MMP_RS02555 at 477.503 kb on - strand, within MMP_RS02555 at 477.504 kb on + strand, within MMP_RS02555 at 477.504 kb on + strand, within MMP_RS02555 at 477.504 kb on + strand, within MMP_RS02555 at 477.571 kb on - strand, within MMP_RS02555 at 477.604 kb on - strand, within MMP_RS02555 at 477.726 kb on - strand, within MMP_RS02555 at 477.857 kb on + strand, within MMP_RS02555 at 477.868 kb on + strand, within MMP_RS02555 at 477.925 kb on - strand, within MMP_RS02555 at 477.926 kb on + strand, within MMP_RS02555 at 477.947 kb on + strand, within MMP_RS02555 at 477.949 kb on - strand, within MMP_RS02555 at 477.949 kb on - strand, within MMP_RS02555 at 477.949 kb on - strand, within MMP_RS02555 at 477.950 kb on + strand, within MMP_RS02555 at 477.950 kb on + strand, within MMP_RS02555 at 477.953 kb on - strand, within MMP_RS02555 at 477.953 kb on - strand, within MMP_RS02555 at 477.954 kb on + strand, within MMP_RS02555 at 477.956 kb on - strand, within MMP_RS02555 at 477.957 kb on + strand, within MMP_RS02555 at 477.957 kb on + strand, within MMP_RS02555 at 477.957 kb on + strand, within MMP_RS02555 at 477.958 kb on - strand, within MMP_RS02555 at 477.958 kb on - strand, within MMP_RS02555 at 477.958 kb on - strand, within MMP_RS02555 at 477.958 kb on - strand, within MMP_RS02555 at 477.958 kb on - strand, within MMP_RS02555 at 477.959 kb on + strand, within MMP_RS02555 at 477.959 kb on + strand, within MMP_RS02555 at 477.959 kb on + strand, within MMP_RS02555 at 477.959 kb on + strand, within MMP_RS02555 at 477.959 kb on + strand, within MMP_RS02555 at 477.994 kb on - strand, within MMP_RS02555 at 477.995 kb on + strand, within MMP_RS02555 at 477.996 kb on - strand, within MMP_RS02555 at 477.997 kb on + strand, within MMP_RS02555 at 478.027 kb on + strand at 478.075 kb on - strand at 478.078 kb on - strand at 478.078 kb on - strand at 478.162 kb on - strand at 478.176 kb on - strand at 478.229 kb on - strand at 478.241 kb on + strand at 478.255 kb on + strand at 478.314 kb on + strand at 478.339 kb on - strand at 478.343 kb on - strand at 478.417 kb on + strand at 478.492 kb on - strand at 478.495 kb on + strand at 478.495 kb on + strand at 478.602 kb on + strand at 478.623 kb on - strand, within MMP_RS02560 at 478.631 kb on - strand, within MMP_RS02560 at 478.637 kb on + strand, within MMP_RS02560 at 478.638 kb on - strand, within MMP_RS02560 at 478.639 kb on + strand, within MMP_RS02560 at 478.647 kb on - strand, within MMP_RS02560 at 478.722 kb on + strand, within MMP_RS02560 at 478.722 kb on + strand, within MMP_RS02560 at 478.722 kb on + strand, within MMP_RS02560 at 478.730 kb on - strand, within MMP_RS02560 at 478.730 kb on - strand, within MMP_RS02560 at 478.731 kb on + strand, within MMP_RS02560 at 478.731 kb on + strand, within MMP_RS02560 at 478.778 kb on - strand, within MMP_RS02560 at 478.856 kb on - strand, within MMP_RS02560 at 478.870 kb on + strand, within MMP_RS02560 at 478.896 kb on - strand, within MMP_RS02560 at 478.897 kb on + strand, within MMP_RS02560 at 478.899 kb on + strand, within MMP_RS02560 at 478.899 kb on + strand, within MMP_RS02560 at 478.970 kb on - strand at 479.027 kb on - strand, within MMP_RS02565 at 479.028 kb on + strand, within MMP_RS02565 at 479.036 kb on - strand, within MMP_RS02565 at 479.036 kb on - strand, within MMP_RS02565 at 479.037 kb on + strand, within MMP_RS02565 at 479.042 kb on - strand, within MMP_RS02565 at 479.045 kb on - strand, within MMP_RS02565 at 479.046 kb on + strand, within MMP_RS02565 at 479.046 kb on + strand, within MMP_RS02565 at 479.060 kb on + strand, within MMP_RS02565 at 479.062 kb on - strand, within MMP_RS02565 at 479.062 kb on - strand, within MMP_RS02565 at 479.063 kb on + strand, within MMP_RS02565 at 479.064 kb on - strand, within MMP_RS02565 at 479.064 kb on - strand, within MMP_RS02565 at 479.065 kb on + strand, within MMP_RS02565 at 479.076 kb on + strand, within MMP_RS02565 at 479.135 kb on - strand, within MMP_RS02565 at 479.136 kb on + strand, within MMP_RS02565 at 479.137 kb on - strand, within MMP_RS02565 at 479.138 kb on + strand, within MMP_RS02565 at 479.157 kb on - strand, within MMP_RS02565
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 475,271 - -1.2 475,350 - -1.7 475,413 + -0.8 475,414 - -2.8 475,415 + +1.7 475,459 + MMP_RS02545 0.20 +1.3 475,480 + MMP_RS02545 0.25 -0.3 475,504 + MMP_RS02545 0.31 -1.3 475,548 - MMP_RS02545 0.42 -1.8 475,605 + MMP_RS02545 0.55 +1.5 475,710 - MMP_RS02545 0.81 -0.8 475,724 + MMP_RS02545 0.84 +0.6 475,800 - +1.7 475,847 - -2.6 475,847 - +0.4 475,847 - -0.8 475,848 + +1.3 475,848 + -0.9 475,848 + +0.5 475,849 - -1.4 475,849 - -0.1 475,850 + -2.9 475,853 - +1.0 475,853 - -2.5 475,853 - -1.8 475,854 + -0.3 475,854 + -1.6 475,855 - -2.3 475,856 + +0.6 475,917 - +1.5 475,917 - -1.6 475,928 + +0.0 475,937 - -1.6 475,938 + +0.6 475,942 + +0.5 475,951 + -1.8 476,012 + -0.2 476,040 - -0.6 476,099 + MMP_RS02550 0.29 +0.9 476,116 + MMP_RS02550 0.37 +1.5 476,127 - MMP_RS02550 0.42 -1.0 476,129 - MMP_RS02550 0.43 -1.2 476,170 + MMP_RS02550 0.62 -1.0 476,170 + MMP_RS02550 0.62 +0.5 476,179 - MMP_RS02550 0.67 +2.3 476,253 + +0.9 476,255 + -1.7 476,290 + -1.1 476,314 + +1.5 476,337 - +0.3 476,377 - -0.0 476,408 - -0.9 476,409 + +0.2 476,445 - +1.0 476,446 + -0.1 476,453 + MMP_RS02555 0.10 +1.4 476,477 - MMP_RS02555 0.12 -3.9 476,478 + MMP_RS02555 0.12 -0.1 476,478 + MMP_RS02555 0.12 -0.7 476,478 + MMP_RS02555 0.12 +0.1 476,479 - MMP_RS02555 0.12 -0.9 476,510 + MMP_RS02555 0.13 -2.5 476,529 - MMP_RS02555 0.14 -0.2 476,562 + MMP_RS02555 0.16 -1.6 476,620 + MMP_RS02555 0.19 -1.0 476,648 + MMP_RS02555 0.20 -1.0 476,652 - MMP_RS02555 0.21 +0.4 476,685 - MMP_RS02555 0.22 +0.9 476,711 + MMP_RS02555 0.24 +0.3 476,714 + MMP_RS02555 0.24 -1.2 476,773 - MMP_RS02555 0.27 -2.6 476,799 - MMP_RS02555 0.28 -0.4 476,888 - MMP_RS02555 0.33 +0.7 476,894 - MMP_RS02555 0.33 +0.1 476,894 - MMP_RS02555 0.33 -0.4 476,895 + MMP_RS02555 0.33 +1.6 476,897 + +2.9 476,897 + MMP_RS02555 0.33 -1.5 476,929 + MMP_RS02555 0.35 +1.4 476,931 - MMP_RS02555 0.35 -4.0 476,946 - MMP_RS02555 0.36 +0.8 476,947 + MMP_RS02555 0.36 +0.0 476,960 + MMP_RS02555 0.36 -1.1 476,983 + MMP_RS02555 0.37 -0.3 477,048 - MMP_RS02555 0.41 -0.8 477,106 + MMP_RS02555 0.44 -2.8 477,106 + MMP_RS02555 0.44 +0.4 477,106 + MMP_RS02555 0.44 +1.6 477,110 - MMP_RS02555 0.44 -0.7 477,111 + MMP_RS02555 0.44 -2.5 477,111 + MMP_RS02555 0.44 +0.2 477,118 + MMP_RS02555 0.44 -2.1 477,153 - MMP_RS02555 0.46 +0.5 477,160 + MMP_RS02555 0.47 -0.1 477,166 - MMP_RS02555 0.47 -3.1 477,180 - MMP_RS02555 0.48 +1.3 477,184 - MMP_RS02555 0.48 -1.3 477,205 + MMP_RS02555 0.49 +0.3 477,218 - MMP_RS02555 0.50 +0.5 477,227 - MMP_RS02555 0.50 +1.5 477,228 + MMP_RS02555 0.50 -0.9 477,228 + MMP_RS02555 0.50 +2.4 477,274 + MMP_RS02555 0.52 +0.6 477,297 - MMP_RS02555 0.54 -0.5 477,300 - MMP_RS02555 0.54 +0.1 477,300 - MMP_RS02555 0.54 +0.2 477,400 + MMP_RS02555 0.59 +0.5 477,400 + MMP_RS02555 0.59 -1.0 477,427 - MMP_RS02555 0.60 +0.2 477,428 + MMP_RS02555 0.60 -0.5 477,475 - MMP_RS02555 0.63 -0.8 477,496 + MMP_RS02555 0.64 -0.9 477,503 - MMP_RS02555 0.64 -0.5 477,503 - MMP_RS02555 0.64 +1.0 477,504 + MMP_RS02555 0.64 +0.8 477,504 + MMP_RS02555 0.64 -0.2 477,504 + MMP_RS02555 0.64 -2.0 477,571 - MMP_RS02555 0.68 +1.4 477,604 - MMP_RS02555 0.69 +0.6 477,726 - MMP_RS02555 0.76 -4.0 477,857 + MMP_RS02555 0.82 +0.5 477,868 + MMP_RS02555 0.83 +0.8 477,925 - MMP_RS02555 0.86 -0.1 477,926 + MMP_RS02555 0.86 -3.1 477,947 + MMP_RS02555 0.87 -0.8 477,949 - MMP_RS02555 0.87 +1.6 477,949 - MMP_RS02555 0.87 +1.9 477,949 - MMP_RS02555 0.87 +1.0 477,950 + MMP_RS02555 0.87 -2.1 477,950 + MMP_RS02555 0.87 -4.5 477,953 - MMP_RS02555 0.87 +2.1 477,953 - MMP_RS02555 0.87 +0.8 477,954 + MMP_RS02555 0.87 -0.1 477,956 - MMP_RS02555 0.87 -3.5 477,957 + MMP_RS02555 0.87 +0.0 477,957 + MMP_RS02555 0.87 +0.4 477,957 + MMP_RS02555 0.87 +1.5 477,958 - MMP_RS02555 0.87 -2.3 477,958 - MMP_RS02555 0.87 +1.9 477,958 - MMP_RS02555 0.87 +0.7 477,958 - MMP_RS02555 0.87 +0.4 477,958 - MMP_RS02555 0.87 -1.5 477,959 + MMP_RS02555 0.87 -0.3 477,959 + MMP_RS02555 0.87 -3.3 477,959 + MMP_RS02555 0.87 -0.1 477,959 + MMP_RS02555 0.87 +0.4 477,959 + MMP_RS02555 0.87 +0.0 477,994 - MMP_RS02555 0.89 -0.1 477,995 + MMP_RS02555 0.89 +0.6 477,996 - MMP_RS02555 0.89 +0.9 477,997 + MMP_RS02555 0.89 -1.3 478,027 + +1.4 478,075 - -4.1 478,078 - +1.1 478,078 - -2.0 478,162 - +0.1 478,176 - -3.3 478,229 - +0.6 478,241 + -0.2 478,255 + +0.3 478,314 + -2.2 478,339 - +1.1 478,343 - +0.8 478,417 + -0.8 478,492 - +1.9 478,495 + -0.0 478,495 + -3.4 478,602 + -1.2 478,623 - MMP_RS02560 0.14 -0.7 478,631 - MMP_RS02560 0.16 +0.3 478,637 + MMP_RS02560 0.17 -0.1 478,638 - MMP_RS02560 0.17 -1.9 478,639 + MMP_RS02560 0.18 -1.0 478,647 - MMP_RS02560 0.20 +1.7 478,722 + MMP_RS02560 0.39 -2.1 478,722 + MMP_RS02560 0.39 +0.9 478,722 + MMP_RS02560 0.39 -0.9 478,730 - MMP_RS02560 0.41 +1.7 478,730 - MMP_RS02560 0.41 +0.3 478,731 + MMP_RS02560 0.42 -1.1 478,731 + MMP_RS02560 0.42 -1.6 478,778 - MMP_RS02560 0.54 -0.1 478,856 - MMP_RS02560 0.74 -1.9 478,870 + MMP_RS02560 0.78 -0.7 478,896 - MMP_RS02560 0.85 -3.7 478,897 + MMP_RS02560 0.85 +0.5 478,899 + MMP_RS02560 0.85 -4.1 478,899 + MMP_RS02560 0.85 +0.1 478,970 - -0.4 479,027 - MMP_RS02565 0.27 -2.3 479,028 + MMP_RS02565 0.28 +1.2 479,036 - MMP_RS02565 0.30 -0.9 479,036 - MMP_RS02565 0.30 +1.0 479,037 + MMP_RS02565 0.31 -0.7 479,042 - MMP_RS02565 0.32 +1.4 479,045 - MMP_RS02565 0.33 +1.1 479,046 + MMP_RS02565 0.34 +2.1 479,046 + MMP_RS02565 0.34 -1.2 479,060 + MMP_RS02565 0.38 -1.9 479,062 - MMP_RS02565 0.39 +0.5 479,062 - MMP_RS02565 0.39 -1.8 479,063 + MMP_RS02565 0.39 +0.4 479,064 - MMP_RS02565 0.40 -2.0 479,064 - MMP_RS02565 0.40 +2.6 479,065 + MMP_RS02565 0.40 -0.0 479,076 + MMP_RS02565 0.44 -3.7 479,135 - MMP_RS02565 0.64 -0.9 479,136 + MMP_RS02565 0.64 -1.1 479,137 - MMP_RS02565 0.65 -0.3 479,138 + MMP_RS02565 0.65 -1.1 479,157 - MMP_RS02565 0.71 +1.1
Or see this region's nucleotide sequence