Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02375 and MMP_RS02380 are separated by 156 nucleotides MMP_RS02380 and MMP_RS02385 overlap by 4 nucleotides MMP_RS02385 and MMP_RS02390 are separated by 9 nucleotides MMP_RS02390 and MMP_RS02395 are separated by 178 nucleotides MMP_RS02395 and MMP_RS02400 are separated by 41 nucleotides
MMP_RS02375: MMP_RS02375 - hypothetical protein, at 442,923 to 443,207
_RS02375
MMP_RS02380: MMP_RS02380 - ATP-binding protein, at 443,364 to 444,209
_RS02380
MMP_RS02385: MMP_RS02385 - ATP-binding protein, at 444,206 to 445,063
_RS02385
MMP_RS02390: MMP_RS02390 - NifB/NifX family molybdenum-iron cluster-binding protein, at 445,073 to 445,432
_RS02390
MMP_RS02395: MMP_RS02395 - hypothetical protein, at 445,611 to 445,883
_RS02395
MMP_RS02400: MMP_RS02400 - site-2 protease family protein, at 445,925 to 446,938
_RS02400
Position (kb) 444
445
446 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 443.227 kb on + strand at 443.231 kb on - strand at 443.231 kb on - strand at 443.231 kb on - strand at 443.231 kb on - strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.240 kb on + strand at 443.242 kb on - strand at 443.274 kb on - strand at 443.293 kb on + strand at 443.294 kb on - strand at 443.574 kb on - strand, within MMP_RS02380 at 443.575 kb on + strand, within MMP_RS02380 at 443.769 kb on + strand, within MMP_RS02380 at 443.807 kb on - strand, within MMP_RS02380 at 443.854 kb on - strand, within MMP_RS02380 at 443.953 kb on + strand, within MMP_RS02380 at 444.023 kb on - strand, within MMP_RS02380 at 444.081 kb on - strand, within MMP_RS02380 at 444.241 kb on - strand at 444.300 kb on - strand, within MMP_RS02385 at 444.305 kb on + strand, within MMP_RS02385 at 444.535 kb on - strand, within MMP_RS02385 at 444.536 kb on + strand, within MMP_RS02385 at 444.578 kb on + strand, within MMP_RS02385 at 444.578 kb on + strand, within MMP_RS02385 at 444.606 kb on + strand, within MMP_RS02385 at 444.828 kb on + strand, within MMP_RS02385 at 444.964 kb on + strand, within MMP_RS02385 at 444.964 kb on + strand, within MMP_RS02385 at 445.010 kb on + strand at 445.014 kb on + strand at 445.050 kb on + strand at 445.057 kb on - strand at 445.078 kb on - strand at 445.223 kb on + strand, within MMP_RS02390 at 445.236 kb on + strand, within MMP_RS02390 at 445.309 kb on - strand, within MMP_RS02390 at 445.353 kb on + strand, within MMP_RS02390 at 445.355 kb on + strand, within MMP_RS02390 at 445.561 kb on + strand at 445.568 kb on - strand at 445.570 kb on - strand at 445.623 kb on - strand at 445.638 kb on - strand at 445.691 kb on - strand, within MMP_RS02395 at 445.692 kb on + strand, within MMP_RS02395 at 445.692 kb on + strand, within MMP_RS02395 at 445.719 kb on + strand, within MMP_RS02395 at 445.722 kb on - strand, within MMP_RS02395 at 445.734 kb on - strand, within MMP_RS02395 at 445.764 kb on - strand, within MMP_RS02395 at 445.772 kb on - strand, within MMP_RS02395 at 445.773 kb on + strand, within MMP_RS02395 at 445.773 kb on + strand, within MMP_RS02395 at 445.802 kb on + strand, within MMP_RS02395 at 445.809 kb on - strand, within MMP_RS02395 at 445.815 kb on - strand, within MMP_RS02395 at 445.816 kb on + strand, within MMP_RS02395 at 445.839 kb on + strand, within MMP_RS02395 at 445.947 kb on + strand at 445.951 kb on - strand at 445.979 kb on + strand at 445.985 kb on - strand at 446.030 kb on + strand, within MMP_RS02400
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 443,227 + -2.1 443,231 - +0.1 443,231 - +0.0 443,231 - -3.9 443,231 - -0.1 443,232 + -1.5 443,232 + -1.0 443,232 + -0.1 443,232 + +0.5 443,232 + -0.8 443,232 + -1.9 443,240 + +0.2 443,242 - +1.1 443,274 - -1.1 443,293 + -0.1 443,294 - -2.9 443,574 - MMP_RS02380 0.25 -1.3 443,575 + MMP_RS02380 0.25 +1.4 443,769 + MMP_RS02380 0.48 +0.0 443,807 - MMP_RS02380 0.52 -0.5 443,854 - MMP_RS02380 0.58 +1.5 443,953 + MMP_RS02380 0.70 -4.4 444,023 - MMP_RS02380 0.78 +1.2 444,081 - MMP_RS02380 0.85 +1.0 444,241 - -3.2 444,300 - MMP_RS02385 0.11 -0.7 444,305 + MMP_RS02385 0.12 -3.2 444,535 - MMP_RS02385 0.38 +0.4 444,536 + MMP_RS02385 0.38 -0.3 444,578 + MMP_RS02385 0.43 -2.3 444,578 + MMP_RS02385 0.43 -0.4 444,606 + MMP_RS02385 0.47 +0.1 444,828 + MMP_RS02385 0.72 -0.8 444,964 + MMP_RS02385 0.88 +0.0 444,964 + MMP_RS02385 0.88 -0.3 445,010 + +2.0 445,014 + -2.9 445,050 + -3.4 445,057 - -0.0 445,078 - -0.6 445,223 + MMP_RS02390 0.42 -3.5 445,236 + MMP_RS02390 0.45 -0.8 445,309 - MMP_RS02390 0.66 +0.7 445,353 + MMP_RS02390 0.78 +2.3 445,355 + MMP_RS02390 0.78 +0.1 445,561 + +1.0 445,568 - +1.5 445,570 - -0.2 445,623 - +1.5 445,638 - -2.5 445,691 - MMP_RS02395 0.29 +2.0 445,692 + MMP_RS02395 0.30 +1.7 445,692 + MMP_RS02395 0.30 +0.7 445,719 + MMP_RS02395 0.40 +0.9 445,722 - MMP_RS02395 0.41 +1.6 445,734 - MMP_RS02395 0.45 +0.5 445,764 - MMP_RS02395 0.56 -0.9 445,772 - MMP_RS02395 0.59 -0.7 445,773 + MMP_RS02395 0.59 +1.4 445,773 + MMP_RS02395 0.59 +0.9 445,802 + MMP_RS02395 0.70 +0.9 445,809 - MMP_RS02395 0.73 -0.4 445,815 - MMP_RS02395 0.75 +0.3 445,816 + MMP_RS02395 0.75 +0.1 445,839 + MMP_RS02395 0.84 -0.1 445,947 + +1.1 445,951 - -1.0 445,979 + +1.6 445,985 - -0.7 446,030 + MMP_RS02400 0.10 -0.8
Or see this region's nucleotide sequence