Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02235 and MMP_RS02240 are separated by 17 nucleotides MMP_RS02240 and MMP_RS02245 are separated by 30 nucleotides MMP_RS02245 and MMP_RS02250 are separated by 132 nucleotides
MMP_RS02235: MMP_RS02235 - CBS domain-containing protein, at 422,287 to 423,252
_RS02235
MMP_RS02240: MMP_RS02240 - methanogenesis marker 7 protein, at 423,270 to 424,184
_RS02240
MMP_RS02245: MMP_RS02245 - hypothetical protein, at 424,215 to 424,667
_RS02245
MMP_RS02250: MMP_RS02250 - FRG domain-containing protein, at 424,800 to 426,188
_RS02250
Position (kb) 423
424
425 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 422.291 kb on - strand at 422.292 kb on + strand at 422.321 kb on - strand at 422.323 kb on - strand at 422.323 kb on - strand at 422.324 kb on + strand at 422.324 kb on + strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.325 kb on - strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.326 kb on + strand at 422.352 kb on - strand at 422.356 kb on - strand at 422.356 kb on - strand at 422.357 kb on + strand at 422.357 kb on + strand at 422.360 kb on - strand at 422.368 kb on - strand at 422.368 kb on - strand at 422.372 kb on - strand at 422.376 kb on - strand at 422.376 kb on - strand at 422.456 kb on - strand, within MMP_RS02235 at 422.456 kb on - strand, within MMP_RS02235 at 422.457 kb on + strand, within MMP_RS02235 at 422.471 kb on - strand, within MMP_RS02235 at 422.472 kb on + strand, within MMP_RS02235 at 422.518 kb on + strand, within MMP_RS02235 at 422.554 kb on + strand, within MMP_RS02235 at 422.623 kb on + strand, within MMP_RS02235 at 422.651 kb on - strand, within MMP_RS02235 at 422.794 kb on + strand, within MMP_RS02235 at 422.829 kb on - strand, within MMP_RS02235 at 422.863 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235 at 422.938 kb on - strand, within MMP_RS02235 at 423.055 kb on + strand, within MMP_RS02235 at 423.071 kb on - strand, within MMP_RS02235 at 423.076 kb on + strand, within MMP_RS02235 at 423.090 kb on + strand, within MMP_RS02235 at 423.126 kb on + strand, within MMP_RS02235 at 423.126 kb on + strand, within MMP_RS02235 at 423.191 kb on - strand at 423.282 kb on + strand at 423.282 kb on + strand at 423.284 kb on - strand at 423.285 kb on + strand at 423.445 kb on + strand, within MMP_RS02240 at 423.777 kb on - strand, within MMP_RS02240 at 423.885 kb on + strand, within MMP_RS02240 at 423.959 kb on + strand, within MMP_RS02240 at 423.959 kb on + strand, within MMP_RS02240 at 424.077 kb on - strand, within MMP_RS02240 at 424.098 kb on - strand at 424.099 kb on + strand at 424.104 kb on + strand at 424.183 kb on + strand at 424.426 kb on - strand, within MMP_RS02245 at 424.427 kb on + strand, within MMP_RS02245 at 424.435 kb on + strand, within MMP_RS02245 at 424.511 kb on - strand, within MMP_RS02245 at 424.517 kb on + strand, within MMP_RS02245 at 424.657 kb on - strand at 424.657 kb on - strand at 424.657 kb on - strand at 424.659 kb on - strand at 424.744 kb on - strand at 424.745 kb on + strand at 425.010 kb on + strand, within MMP_RS02250 at 425.092 kb on - strand, within MMP_RS02250 at 425.128 kb on - strand, within MMP_RS02250 at 425.145 kb on + strand, within MMP_RS02250 at 425.165 kb on - strand, within MMP_RS02250 at 425.166 kb on + strand, within MMP_RS02250
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 422,291 - -1.2 422,292 + -3.8 422,321 - -0.6 422,323 - -1.9 422,323 - -1.6 422,324 + -1.2 422,324 + -2.8 422,325 - -4.6 422,325 - -2.3 422,325 - -3.1 422,326 + -1.8 422,326 + -3.4 422,326 + -2.6 422,352 - -0.6 422,356 - -1.6 422,356 - -4.3 422,357 + +0.3 422,357 + -4.1 422,360 - -1.4 422,368 - -1.8 422,368 - -1.7 422,372 - -1.7 422,376 - -1.6 422,376 - -2.0 422,456 - MMP_RS02235 0.17 -1.8 422,456 - MMP_RS02235 0.17 -4.3 422,457 + MMP_RS02235 0.18 -0.9 422,471 - MMP_RS02235 0.19 -2.5 422,472 + MMP_RS02235 0.19 -2.8 422,518 + MMP_RS02235 0.24 -0.5 422,554 + MMP_RS02235 0.28 -1.2 422,623 + MMP_RS02235 0.35 -1.5 422,651 - MMP_RS02235 0.38 -2.1 422,794 + MMP_RS02235 0.52 -2.5 422,829 - MMP_RS02235 0.56 -0.7 422,863 - MMP_RS02235 0.60 -2.4 422,938 - MMP_RS02235 0.67 -1.0 422,938 - MMP_RS02235 0.67 -2.1 423,055 + MMP_RS02235 0.80 -2.2 423,071 - MMP_RS02235 0.81 -0.6 423,076 + MMP_RS02235 0.82 -2.0 423,090 + MMP_RS02235 0.83 -1.5 423,126 + MMP_RS02235 0.87 -2.4 423,126 + MMP_RS02235 0.87 -1.4 423,191 - -2.9 423,282 + -1.3 423,282 + +0.4 423,284 - -1.5 423,285 + -1.9 423,445 + MMP_RS02240 0.19 +0.6 423,777 - MMP_RS02240 0.55 +0.1 423,885 + MMP_RS02240 0.67 -0.3 423,959 + MMP_RS02240 0.75 -1.8 423,959 + MMP_RS02240 0.75 -0.5 424,077 - MMP_RS02240 0.88 -0.6 424,098 - -2.4 424,099 + +0.8 424,104 + -1.5 424,183 + -1.4 424,426 - MMP_RS02245 0.47 -2.3 424,427 + MMP_RS02245 0.47 -0.0 424,435 + MMP_RS02245 0.49 -1.8 424,511 - MMP_RS02245 0.65 -3.8 424,517 + MMP_RS02245 0.67 +1.0 424,657 - -1.5 424,657 - +1.4 424,657 - -0.6 424,659 - -1.6 424,744 - -0.6 424,745 + +1.9 425,010 + MMP_RS02250 0.15 -4.4 425,092 - MMP_RS02250 0.21 -0.7 425,128 - MMP_RS02250 0.24 +1.1 425,145 + MMP_RS02250 0.25 -2.0 425,165 - MMP_RS02250 0.26 -1.0 425,166 + MMP_RS02250 0.26 +0.2
Or see this region's nucleotide sequence