Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02100 and MMP_RS02105 are separated by 58 nucleotides MMP_RS02105 and MMP_RS02110 are separated by 15 nucleotides MMP_RS02110 and MMP_RS02115 are separated by 241 nucleotides
MMP_RS02100: MMP_RS02100 - metallophosphoesterase, at 391,261 to 391,776
_RS02100
MMP_RS02105: MMP_RS02105 - uroporphyrinogen-III synthase, at 391,835 to 392,569
_RS02105
MMP_RS02110: MMP_RS02110 - hypothetical protein, at 392,585 to 392,863
_RS02110
MMP_RS02115: MMP_RS02115 - phosphopyruvate hydratase, at 393,105 to 394,397
_RS02115
Position (kb) 392
393 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 391.729 kb on - strand at 391.731 kb on - strand at 391.738 kb on - strand at 391.743 kb on + strand at 391.762 kb on - strand at 391.789 kb on - strand at 391.789 kb on - strand at 391.801 kb on - strand at 391.822 kb on + strand at 391.852 kb on - strand at 391.881 kb on - strand at 391.952 kb on - strand at 391.959 kb on - strand at 392.032 kb on - strand at 392.074 kb on - strand at 392.169 kb on + strand at 392.433 kb on - strand at 392.616 kb on - strand, within MMP_RS02110 at 392.616 kb on - strand, within MMP_RS02110 at 392.876 kb on - strand at 392.896 kb on + strand at 392.910 kb on + strand at 392.920 kb on + strand at 393.010 kb on - strand at 393.515 kb on - strand at 393.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 391,729 - -0.9 391,731 - +0.7 391,738 - +0.6 391,743 + +1.0 391,762 - -3.3 391,789 - -4.6 391,789 - -0.9 391,801 - +0.6 391,822 + -2.6 391,852 - -1.8 391,881 - -2.0 391,952 - -0.4 391,959 - -1.2 392,032 - -0.3 392,074 - -1.4 392,169 + +1.4 392,433 - -0.6 392,616 - MMP_RS02110 0.11 -0.2 392,616 - MMP_RS02110 0.11 -2.0 392,876 - -1.5 392,896 + -1.2 392,910 + +0.1 392,920 + +0.4 393,010 - +2.1 393,515 - -1.0 393,541 + -0.9
Or see this region's nucleotide sequence