Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02000 and MMP_RS02005 are separated by 464 nucleotides MMP_RS02005 and MMP_RS02010 are separated by 5 nucleotides MMP_RS02010 and MMP_RS02015 are separated by 28 nucleotides
MMP_RS02000: MMP_RS02000 - radical SAM protein, at 371,172 to 372,545
_RS02000
MMP_RS02005: MMP_RS02005 - RNA ligase, at 373,010 to 374,194
_RS02005
MMP_RS02010: MMP_RS02010 - PHP domain-containing protein, at 374,200 to 374,844
_RS02010
MMP_RS02015: MMP_RS02015 - amidohydrolase, at 374,873 to 376,156
_RS02015
Position (kb) 373
374
375 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 372.048 kb on - strand, within MMP_RS02000 at 372.232 kb on + strand, within MMP_RS02000 at 372.464 kb on + strand at 372.468 kb on + strand at 372.543 kb on - strand at 372.626 kb on + strand at 372.626 kb on + strand at 372.628 kb on - strand at 372.628 kb on - strand at 372.628 kb on - strand at 372.629 kb on + strand at 372.638 kb on - strand at 372.675 kb on - strand at 372.676 kb on + strand at 372.676 kb on + strand at 372.679 kb on - strand at 372.709 kb on - strand at 372.756 kb on + strand at 372.892 kb on - strand at 372.899 kb on + strand at 372.946 kb on - strand at 372.947 kb on + strand at 372.947 kb on + strand at 372.950 kb on - strand at 372.950 kb on - strand at 372.951 kb on + strand at 372.951 kb on + strand at 372.954 kb on - strand at 372.954 kb on - strand at 372.955 kb on + strand at 372.959 kb on - strand at 372.963 kb on - strand at 372.964 kb on + strand at 372.964 kb on + strand at 372.964 kb on + strand at 372.964 kb on + strand at 372.964 kb on + strand at 372.964 kb on + strand at 372.965 kb on - strand at 372.965 kb on - strand at 372.967 kb on - strand at 372.968 kb on + strand at 372.968 kb on + strand at 372.972 kb on + strand at 372.997 kb on + strand at 373.046 kb on + strand at 373.084 kb on - strand at 373.088 kb on - strand at 373.088 kb on - strand at 373.089 kb on + strand at 373.104 kb on - strand at 373.129 kb on - strand, within MMP_RS02005 at 373.155 kb on + strand, within MMP_RS02005 at 373.421 kb on + strand, within MMP_RS02005 at 373.421 kb on + strand, within MMP_RS02005 at 373.426 kb on - strand, within MMP_RS02005 at 373.427 kb on + strand, within MMP_RS02005 at 373.517 kb on - strand, within MMP_RS02005 at 373.535 kb on + strand, within MMP_RS02005 at 373.574 kb on + strand, within MMP_RS02005 at 373.574 kb on + strand, within MMP_RS02005 at 373.575 kb on - strand, within MMP_RS02005 at 373.692 kb on - strand, within MMP_RS02005 at 373.865 kb on - strand, within MMP_RS02005 at 374.061 kb on - strand, within MMP_RS02005 at 374.141 kb on - strand at 374.181 kb on - strand at 374.472 kb on - strand, within MMP_RS02010 at 374.474 kb on - strand, within MMP_RS02010 at 374.475 kb on + strand, within MMP_RS02010 at 374.476 kb on - strand, within MMP_RS02010 at 374.477 kb on + strand, within MMP_RS02010 at 374.485 kb on - strand, within MMP_RS02010 at 374.487 kb on - strand, within MMP_RS02010 at 374.492 kb on + strand, within MMP_RS02010 at 374.532 kb on - strand, within MMP_RS02010 at 374.576 kb on - strand, within MMP_RS02010 at 374.577 kb on + strand, within MMP_RS02010 at 374.599 kb on - strand, within MMP_RS02010 at 374.599 kb on - strand, within MMP_RS02010 at 374.698 kb on - strand, within MMP_RS02010 at 374.869 kb on - strand at 374.875 kb on + strand at 374.902 kb on - strand at 374.914 kb on - strand at 374.918 kb on + strand at 374.921 kb on - strand at 374.943 kb on - strand at 374.963 kb on - strand at 374.965 kb on - strand at 374.982 kb on + strand at 375.019 kb on - strand, within MMP_RS02015 at 375.038 kb on + strand, within MMP_RS02015 at 375.074 kb on - strand, within MMP_RS02015 at 375.077 kb on + strand, within MMP_RS02015
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 372,048 - MMP_RS02000 0.64 +0.2 372,232 + MMP_RS02000 0.77 +1.5 372,464 + +0.7 372,468 + -2.4 372,543 - +0.3 372,626 + -3.0 372,626 + -2.1 372,628 - +0.4 372,628 - +0.5 372,628 - -0.5 372,629 + -3.7 372,638 - +1.2 372,675 - +1.2 372,676 + +0.3 372,676 + +0.6 372,679 - +1.6 372,709 - -0.8 372,756 + +2.1 372,892 - -1.3 372,899 + +1.6 372,946 - +0.2 372,947 + +1.6 372,947 + +1.8 372,950 - -0.4 372,950 - +0.7 372,951 + -2.0 372,951 + +0.8 372,954 - -1.4 372,954 - -0.2 372,955 + +1.2 372,959 - +0.6 372,963 - -2.0 372,964 + -4.4 372,964 + -1.0 372,964 + -2.0 372,964 + -0.7 372,964 + -0.3 372,964 + -3.1 372,965 - -2.2 372,965 - -1.8 372,967 - +1.1 372,968 + +0.4 372,968 + +0.5 372,972 + +1.0 372,997 + -2.4 373,046 + -2.2 373,084 - +1.6 373,088 - +0.5 373,088 - +1.0 373,089 + +1.8 373,104 - -3.4 373,129 - MMP_RS02005 0.10 -0.8 373,155 + MMP_RS02005 0.12 +1.9 373,421 + MMP_RS02005 0.35 -1.2 373,421 + MMP_RS02005 0.35 -0.2 373,426 - MMP_RS02005 0.35 -2.3 373,427 + MMP_RS02005 0.35 +1.0 373,517 - MMP_RS02005 0.43 -1.0 373,535 + MMP_RS02005 0.44 -0.5 373,574 + MMP_RS02005 0.48 -2.0 373,574 + MMP_RS02005 0.48 +0.3 373,575 - MMP_RS02005 0.48 -1.9 373,692 - MMP_RS02005 0.58 -1.1 373,865 - MMP_RS02005 0.72 +0.8 374,061 - MMP_RS02005 0.89 -3.5 374,141 - +1.3 374,181 - +0.8 374,472 - MMP_RS02010 0.42 -1.3 374,474 - MMP_RS02010 0.42 -3.1 374,475 + MMP_RS02010 0.43 -2.8 374,476 - MMP_RS02010 0.43 -2.7 374,477 + MMP_RS02010 0.43 -3.0 374,485 - MMP_RS02010 0.44 -1.7 374,487 - MMP_RS02010 0.44 -4.1 374,492 + MMP_RS02010 0.45 -3.0 374,532 - MMP_RS02010 0.51 -2.1 374,576 - MMP_RS02010 0.58 -1.4 374,577 + MMP_RS02010 0.58 -0.6 374,599 - MMP_RS02010 0.62 -1.4 374,599 - MMP_RS02010 0.62 -2.1 374,698 - MMP_RS02010 0.77 -1.2 374,869 - -1.2 374,875 + -0.7 374,902 - -0.0 374,914 - +1.6 374,918 + -0.5 374,921 - -0.5 374,943 - -0.5 374,963 - -1.3 374,965 - -1.3 374,982 + -1.2 375,019 - MMP_RS02015 0.11 +0.7 375,038 + MMP_RS02015 0.13 +0.3 375,074 - MMP_RS02015 0.16 -1.9 375,077 + MMP_RS02015 0.16 +0.8
Or see this region's nucleotide sequence