Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01855 and MMP_RS01860 are separated by 225 nucleotides MMP_RS01860 and MMP_RS01865 are separated by 90 nucleotides MMP_RS01865 and MMP_RS01870 are separated by 126 nucleotides
MMP_RS01855: MMP_RS01855 - 2-hydroxyacyl-CoA dehydratase family protein, at 342,467 to 343,588
_RS01855
MMP_RS01860: MMP_RS01860 - hypothetical protein, at 343,814 to 344,128
_RS01860
MMP_RS01865: MMP_RS01865 - GPR1/FUN34/YaaH family transporter, at 344,219 to 344,812
_RS01865
MMP_RS01870: MMP_RS01870 - acyl-CoA dehydratase activase, at 344,939 to 345,679
_RS01870
Position (kb)
344
345 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 343.318 kb on + strand, within MMP_RS01855 at 343.403 kb on - strand, within MMP_RS01855 at 343.488 kb on + strand at 343.527 kb on + strand at 343.646 kb on - strand at 343.657 kb on - strand at 343.657 kb on - strand at 343.657 kb on - strand at 343.658 kb on + strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.666 kb on - strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.667 kb on + strand at 343.669 kb on + strand at 343.669 kb on + strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.676 kb on - strand at 343.677 kb on + strand at 343.688 kb on + strand at 343.849 kb on + strand, within MMP_RS01860 at 343.849 kb on + strand, within MMP_RS01860 at 343.849 kb on + strand, within MMP_RS01860 at 343.849 kb on + strand, within MMP_RS01860 at 343.879 kb on - strand, within MMP_RS01860 at 343.879 kb on - strand, within MMP_RS01860 at 343.896 kb on + strand, within MMP_RS01860 at 343.911 kb on + strand, within MMP_RS01860 at 343.992 kb on + strand, within MMP_RS01860 at 344.004 kb on - strand, within MMP_RS01860 at 344.023 kb on - strand, within MMP_RS01860 at 344.096 kb on - strand, within MMP_RS01860 at 344.099 kb on + strand at 344.103 kb on + strand at 344.115 kb on + strand at 344.128 kb on - strand at 344.182 kb on - strand at 344.182 kb on - strand at 344.182 kb on - strand at 344.183 kb on + strand at 344.184 kb on - strand at 344.184 kb on - strand at 344.185 kb on + strand at 344.186 kb on - strand at 344.187 kb on + strand at 344.187 kb on + strand at 344.388 kb on + strand, within MMP_RS01865 at 344.790 kb on + strand at 344.819 kb on + strand at 344.856 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.858 kb on - strand at 344.859 kb on + strand at 344.859 kb on + strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.860 kb on - strand at 344.861 kb on + strand at 344.861 kb on + strand at 344.873 kb on + strand at 344.884 kb on - strand at 344.910 kb on - strand at 344.913 kb on + strand at 344.924 kb on + strand at 345.036 kb on + strand, within MMP_RS01870 at 345.078 kb on + strand, within MMP_RS01870 at 345.139 kb on + strand, within MMP_RS01870 at 345.140 kb on - strand, within MMP_RS01870 at 345.141 kb on + strand, within MMP_RS01870 at 345.289 kb on - strand, within MMP_RS01870 at 345.372 kb on + strand, within MMP_RS01870 at 345.372 kb on + strand, within MMP_RS01870 at 345.485 kb on - strand, within MMP_RS01870 at 345.560 kb on - strand, within MMP_RS01870 at 345.605 kb on + strand, within MMP_RS01870 at 345.614 kb on - strand at 345.671 kb on - strand at 345.729 kb on - strand at 345.735 kb on - strand at 345.739 kb on - strand at 345.739 kb on - strand at 345.740 kb on + strand at 345.743 kb on - strand at 345.756 kb on + strand at 345.757 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.774 kb on - strand at 345.775 kb on + strand at 345.775 kb on + strand at 345.783 kb on - strand at 345.789 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 343,318 + MMP_RS01855 0.76 +0.9 343,403 - MMP_RS01855 0.83 -1.3 343,488 + +1.1 343,527 + -0.3 343,646 - +0.6 343,657 - -2.7 343,657 - -0.9 343,657 - +1.0 343,658 + +0.3 343,666 - +1.8 343,666 - -1.0 343,666 - -2.4 343,666 - +1.5 343,666 - +1.0 343,666 - +1.9 343,666 - +1.0 343,666 - -1.8 343,667 + +0.3 343,667 + +0.2 343,667 + +0.6 343,667 + +0.1 343,667 + +1.2 343,667 + +1.0 343,667 + +1.1 343,667 + -1.5 343,669 + +1.3 343,669 + -0.5 343,676 - -2.5 343,676 - -1.3 343,676 - -0.3 343,676 - +0.5 343,676 - +1.7 343,676 - +1.8 343,677 + -2.7 343,688 + +0.7 343,849 + MMP_RS01860 0.11 -2.5 343,849 + MMP_RS01860 0.11 -1.6 343,849 + MMP_RS01860 0.11 -1.7 343,849 + MMP_RS01860 0.11 -0.6 343,879 - MMP_RS01860 0.21 -1.2 343,879 - MMP_RS01860 0.21 -1.3 343,896 + MMP_RS01860 0.26 +0.7 343,911 + MMP_RS01860 0.31 +1.1 343,992 + MMP_RS01860 0.57 +1.1 344,004 - MMP_RS01860 0.60 -0.4 344,023 - MMP_RS01860 0.66 +0.9 344,096 - MMP_RS01860 0.90 -0.0 344,099 + -0.9 344,103 + -3.1 344,115 + +0.5 344,128 - +0.7 344,182 - +0.9 344,182 - +1.1 344,182 - +1.1 344,183 + +1.4 344,184 - +1.8 344,184 - +0.7 344,185 + +0.4 344,186 - -3.2 344,187 + -1.1 344,187 + -4.8 344,388 + MMP_RS01865 0.28 -0.5 344,790 + +2.1 344,819 + -0.5 344,856 - -2.2 344,858 - -0.3 344,858 - +0.2 344,858 - -1.1 344,858 - -1.4 344,859 + -2.1 344,859 + -1.2 344,860 - +1.1 344,860 - -0.0 344,860 - -1.9 344,860 - -1.2 344,861 + -0.2 344,861 + +0.2 344,873 + +0.7 344,884 - -2.6 344,910 - +1.6 344,913 + +2.2 344,924 + -0.3 345,036 + MMP_RS01870 0.13 -1.0 345,078 + MMP_RS01870 0.19 -0.4 345,139 + MMP_RS01870 0.27 -0.0 345,140 - MMP_RS01870 0.27 +0.0 345,141 + MMP_RS01870 0.27 +1.9 345,289 - MMP_RS01870 0.47 -0.3 345,372 + MMP_RS01870 0.58 +0.9 345,372 + MMP_RS01870 0.58 +0.6 345,485 - MMP_RS01870 0.74 +0.9 345,560 - MMP_RS01870 0.84 -0.2 345,605 + MMP_RS01870 0.90 +1.0 345,614 - -1.0 345,671 - +0.9 345,729 - +0.5 345,735 - +0.1 345,739 - +0.2 345,739 - +1.5 345,740 + -1.5 345,743 - +1.0 345,756 + +0.1 345,757 - -0.5 345,774 - -1.1 345,774 - +0.2 345,774 - -1.0 345,775 + +0.6 345,775 + -0.9 345,783 - +1.4 345,789 + -2.6
Or see this region's nucleotide sequence