Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01720 and MMP_RS01725 are separated by 9 nucleotides MMP_RS01725 and MMP_RS01730 are separated by 50 nucleotides MMP_RS01730 and MMP_RS01735 are separated by 66 nucleotides
MMP_RS01720: MMP_RS01720 - replication factor C large subunit, at 318,173 to 319,633
_RS01720
MMP_RS01725: MMP_RS01725 - class I SAM-dependent methyltransferase family protein, at 319,643 to 320,671
_RS01725
MMP_RS01730: MMP_RS01730 - hypothetical protein, at 320,722 to 321,102
_RS01730
MMP_RS01735: MMP_RS01735 - type II glyceraldehyde-3-phosphate dehydrogenase, at 321,169 to 322,191
_RS01735
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 319.396 kb on + strand, within MMP_RS01720 at 319.656 kb on + strand at 319.684 kb on + strand at 319.786 kb on - strand, within MMP_RS01725 at 319.831 kb on - strand, within MMP_RS01725 at 319.871 kb on + strand, within MMP_RS01725 at 319.985 kb on - strand, within MMP_RS01725 at 319.999 kb on + strand, within MMP_RS01725 at 320.173 kb on + strand, within MMP_RS01725 at 320.432 kb on + strand, within MMP_RS01725 at 320.584 kb on - strand at 320.594 kb on + strand at 320.650 kb on - strand at 320.695 kb on + strand at 320.696 kb on - strand at 320.697 kb on + strand at 320.698 kb on - strand at 320.698 kb on - strand at 320.709 kb on - strand at 320.734 kb on + strand at 320.743 kb on + strand at 320.844 kb on - strand at 321.020 kb on + strand at 321.023 kb on - strand at 321.024 kb on + strand at 321.308 kb on + strand at 321.319 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 319,396 + MMP_RS01720 0.84 +1.2 319,656 + -1.1 319,684 + -0.2 319,786 - MMP_RS01725 0.14 -0.3 319,831 - MMP_RS01725 0.18 -2.8 319,871 + MMP_RS01725 0.22 +0.1 319,985 - MMP_RS01725 0.33 -1.3 319,999 + MMP_RS01725 0.35 +0.0 320,173 + MMP_RS01725 0.52 -0.1 320,432 + MMP_RS01725 0.77 -0.8 320,584 - -0.1 320,594 + +0.4 320,650 - +0.6 320,695 + +1.1 320,696 - -0.4 320,697 + +1.6 320,698 - +0.8 320,698 - -0.9 320,709 - -2.2 320,734 + +0.9 320,743 + -0.2 320,844 - -2.0 321,020 + -1.0 321,023 - -0.2 321,024 + -2.3 321,308 + +1.5 321,319 - -0.8
Or see this region's nucleotide sequence