Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01680 and MMP_RS01685 overlap by 8 nucleotides MMP_RS01685 and MMP_RS01690 overlap by 4 nucleotides MMP_RS01690 and MMP_RS01695 are separated by 367 nucleotides
MMP_RS01680: MMP_RS01680 - phenylacetate--CoA ligase, at 308,925 to 310,169
_RS01680
MMP_RS01685: MMP_RS01685 - indolepyruvate oxidoreductase subunit beta, at 310,162 to 310,761
_RS01685
MMP_RS01690: MMP_RS01690 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 310,758 to 312,596
_RS01690
MMP_RS01695: MMP_RS01695 - fructose-1 6-bisphosphatase, at 312,964 to 314,115
_RS01695
Position (kb) 310
311
312
313 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 309.821 kb on - strand, within MMP_RS01680 at 309.828 kb on + strand, within MMP_RS01680 at 309.879 kb on + strand, within MMP_RS01680 at 309.882 kb on - strand, within MMP_RS01680 at 309.883 kb on + strand, within MMP_RS01680 at 309.898 kb on - strand, within MMP_RS01680 at 309.903 kb on - strand, within MMP_RS01680 at 309.943 kb on + strand, within MMP_RS01680 at 310.049 kb on - strand at 310.050 kb on + strand at 310.065 kb on - strand at 310.065 kb on - strand at 310.065 kb on - strand at 310.397 kb on - strand, within MMP_RS01685 at 310.486 kb on - strand, within MMP_RS01685 at 310.745 kb on + strand at 310.997 kb on + strand, within MMP_RS01690 at 311.111 kb on - strand, within MMP_RS01690 at 311.229 kb on - strand, within MMP_RS01690 at 311.427 kb on + strand, within MMP_RS01690 at 311.619 kb on - strand, within MMP_RS01690 at 311.620 kb on + strand, within MMP_RS01690 at 311.860 kb on + strand, within MMP_RS01690 at 312.075 kb on - strand, within MMP_RS01690 at 312.107 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.313 kb on - strand, within MMP_RS01690 at 312.314 kb on + strand, within MMP_RS01690 at 312.370 kb on + strand, within MMP_RS01690 at 312.455 kb on - strand at 312.609 kb on - strand at 312.613 kb on + strand at 312.671 kb on + strand at 312.671 kb on + strand at 312.672 kb on - strand at 312.673 kb on + strand at 312.673 kb on + strand at 312.674 kb on - strand at 312.674 kb on - strand at 312.680 kb on + strand at 312.711 kb on + strand at 312.776 kb on + strand at 312.786 kb on + strand at 312.790 kb on - strand at 312.819 kb on - strand at 312.823 kb on - strand at 312.842 kb on - strand at 312.843 kb on + strand at 312.843 kb on + strand at 312.843 kb on + strand at 312.895 kb on + strand at 312.912 kb on - strand at 312.912 kb on - strand at 312.918 kb on - strand at 312.984 kb on + strand at 313.029 kb on + strand at 313.435 kb on - strand at 313.525 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 309,821 - MMP_RS01680 0.72 -1.1 309,828 + MMP_RS01680 0.73 -1.6 309,879 + MMP_RS01680 0.77 +1.2 309,882 - MMP_RS01680 0.77 -1.9 309,883 + MMP_RS01680 0.77 -0.8 309,898 - MMP_RS01680 0.78 -2.6 309,903 - MMP_RS01680 0.79 +0.7 309,943 + MMP_RS01680 0.82 -0.2 310,049 - +0.1 310,050 + -2.8 310,065 - +0.3 310,065 - +0.1 310,065 - +0.4 310,397 - MMP_RS01685 0.39 -1.0 310,486 - MMP_RS01685 0.54 -0.6 310,745 + +0.1 310,997 + MMP_RS01690 0.13 -0.6 311,111 - MMP_RS01690 0.19 +1.4 311,229 - MMP_RS01690 0.26 -0.2 311,427 + MMP_RS01690 0.36 -2.6 311,619 - MMP_RS01690 0.47 -2.1 311,620 + MMP_RS01690 0.47 +0.6 311,860 + MMP_RS01690 0.60 +0.4 312,075 - MMP_RS01690 0.72 -3.1 312,107 - MMP_RS01690 0.73 -0.2 312,313 - MMP_RS01690 0.85 +0.1 312,313 - MMP_RS01690 0.85 -3.2 312,313 - MMP_RS01690 0.85 -0.0 312,314 + MMP_RS01690 0.85 -0.1 312,370 + MMP_RS01690 0.88 -1.3 312,455 - -1.1 312,609 - -3.4 312,613 + -1.9 312,671 + +0.7 312,671 + -3.2 312,672 - +1.6 312,673 + -3.1 312,673 + +0.4 312,674 - +1.4 312,674 - -1.3 312,680 + +0.3 312,711 + +0.6 312,776 + -0.2 312,786 + +1.1 312,790 - +1.0 312,819 - -3.2 312,823 - -0.1 312,842 - -0.4 312,843 + +1.7 312,843 + +0.4 312,843 + -0.2 312,895 + -0.8 312,912 - -0.4 312,912 - -0.5 312,918 - -0.7 312,984 + -1.0 313,029 + +0.2 313,435 - -0.9 313,525 - +0.0
Or see this region's nucleotide sequence