Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01660 and MMP_RS01665 are separated by 54 nucleotides MMP_RS01665 and MMP_RS01670 are separated by 20 nucleotides MMP_RS01670 and MMP_RS01675 are separated by 8 nucleotides MMP_RS01675 and MMP_RS01680 are separated by 314 nucleotides
MMP_RS01660: MMP_RS01660 - hypothetical protein, at 306,663 to 307,247
_RS01660
MMP_RS01665: MMP_RS01665 - DUF2111 domain-containing protein, at 307,302 to 307,685
_RS01665
MMP_RS01670: MMP_RS01670 - methanogenesis marker 5 protein, at 307,706 to 308,146
_RS01670
MMP_RS01675: MMP_RS01675 - hypothetical protein, at 308,155 to 308,610
_RS01675
MMP_RS01680: MMP_RS01680 - phenylacetate--CoA ligase, at 308,925 to 310,169
_RS01680
Position (kb) 308
309 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 307.249 kb on - strand at 307.263 kb on + strand at 307.272 kb on - strand at 307.272 kb on - strand at 307.273 kb on + strand at 307.273 kb on + strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.276 kb on - strand at 307.277 kb on + strand at 307.277 kb on + strand at 307.277 kb on + strand at 307.288 kb on - strand at 307.288 kb on - strand at 307.289 kb on + strand at 307.337 kb on + strand at 307.351 kb on + strand, within MMP_RS01665 at 307.355 kb on - strand, within MMP_RS01665 at 307.363 kb on - strand, within MMP_RS01665 at 307.363 kb on - strand, within MMP_RS01665 at 307.450 kb on + strand, within MMP_RS01665 at 307.609 kb on - strand, within MMP_RS01665 at 307.684 kb on + strand at 308.172 kb on - strand at 308.340 kb on + strand, within MMP_RS01675 at 308.592 kb on + strand at 308.592 kb on + strand at 308.595 kb on + strand at 308.612 kb on - strand at 308.613 kb on + strand at 308.613 kb on + strand at 308.614 kb on - strand at 308.615 kb on + strand at 308.671 kb on - strand at 308.671 kb on - strand at 308.672 kb on + strand at 308.674 kb on + strand at 308.676 kb on + strand at 308.677 kb on - strand at 308.690 kb on - strand at 308.708 kb on - strand at 308.717 kb on + strand at 308.725 kb on - strand at 308.726 kb on + strand at 308.726 kb on + strand at 308.727 kb on - strand at 308.854 kb on - strand at 309.019 kb on - strand at 309.020 kb on + strand at 309.036 kb on - strand at 309.092 kb on - strand, within MMP_RS01680 at 309.097 kb on + strand, within MMP_RS01680 at 309.110 kb on - strand, within MMP_RS01680 at 309.276 kb on + strand, within MMP_RS01680 at 309.344 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.490 kb on + strand, within MMP_RS01680 at 309.509 kb on - strand, within MMP_RS01680
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 307,249 - +0.9 307,263 + +1.5 307,272 - -1.9 307,272 - -1.4 307,273 + +1.1 307,273 + -2.0 307,276 - -1.5 307,276 - -0.5 307,276 - -1.6 307,276 - -2.6 307,276 - +2.1 307,276 - +0.0 307,277 + +0.8 307,277 + -3.0 307,277 + -0.2 307,288 - -0.3 307,288 - +0.1 307,289 + -1.2 307,337 + -0.3 307,351 + MMP_RS01665 0.13 -1.6 307,355 - MMP_RS01665 0.14 -0.2 307,363 - MMP_RS01665 0.16 +0.5 307,363 - MMP_RS01665 0.16 +0.4 307,450 + MMP_RS01665 0.39 -0.9 307,609 - MMP_RS01665 0.80 +2.0 307,684 + -1.2 308,172 - -0.4 308,340 + MMP_RS01675 0.41 -0.7 308,592 + -3.2 308,592 + -0.3 308,595 + -0.4 308,612 - +0.6 308,613 + +1.0 308,613 + -1.1 308,614 - +1.7 308,615 + +1.5 308,671 - +2.0 308,671 - -2.8 308,672 + -2.7 308,674 + +0.7 308,676 + -2.3 308,677 - -1.6 308,690 - +1.3 308,708 - +0.3 308,717 + +1.5 308,725 - -1.7 308,726 + +2.6 308,726 + -2.3 308,727 - +2.3 308,854 - -1.1 309,019 - -1.2 309,020 + +1.7 309,036 - -1.2 309,092 - MMP_RS01680 0.13 +0.7 309,097 + MMP_RS01680 0.14 -0.5 309,110 - MMP_RS01680 0.15 +0.4 309,276 + MMP_RS01680 0.28 +1.9 309,344 - MMP_RS01680 0.34 +0.6 309,489 - MMP_RS01680 0.45 -0.7 309,489 - MMP_RS01680 0.45 +0.7 309,489 - MMP_RS01680 0.45 -0.7 309,490 + MMP_RS01680 0.45 +0.7 309,509 - MMP_RS01680 0.47 +0.5
Or see this region's nucleotide sequence