Experiment: Formate McCas no stress control 2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01630 and MMP_RS01635 are separated by 46 nucleotides MMP_RS01635 and MMP_RS01640 are separated by 5 nucleotides MMP_RS01640 and MMP_RS01645 are separated by 72 nucleotides MMP_RS01645 and MMP_RS01650 are separated by 156 nucleotides
MMP_RS01630: MMP_RS01630 - N-glycosylase/DNA lyase, at 301,670 to 302,428
_RS01630
MMP_RS01635: MMP_RS01635 - thiamine pyrophosphate-dependent enzyme, at 302,475 to 303,335
_RS01635
MMP_RS01640: MMP_RS01640 - 2-oxoacid:acceptor oxidoreductase subunit alpha, at 303,341 to 305,053
_RS01640
MMP_RS01645: MMP_RS01645 - SWIM zinc finger family protein, at 305,126 to 305,815
_RS01645
MMP_RS01650: MMP_RS01650 - sulfurtransferase TusA family protein, at 305,972 to 306,184
_RS01650
Position (kb) 303
304
305
306 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 302.408 kb on - strand at 302.447 kb on - strand at 302.455 kb on + strand at 302.459 kb on + strand at 302.558 kb on + strand at 302.702 kb on + strand, within MMP_RS01635 at 302.748 kb on - strand, within MMP_RS01635 at 302.755 kb on + strand, within MMP_RS01635 at 302.958 kb on + strand, within MMP_RS01635 at 302.966 kb on + strand, within MMP_RS01635 at 302.966 kb on + strand, within MMP_RS01635 at 302.985 kb on - strand, within MMP_RS01635 at 303.047 kb on + strand, within MMP_RS01635 at 303.301 kb on + strand at 303.449 kb on - strand at 303.612 kb on - strand, within MMP_RS01640 at 304.001 kb on + strand, within MMP_RS01640 at 304.051 kb on + strand, within MMP_RS01640 at 304.052 kb on - strand, within MMP_RS01640 at 304.092 kb on + strand, within MMP_RS01640 at 304.160 kb on - strand, within MMP_RS01640 at 304.163 kb on - strand, within MMP_RS01640 at 304.284 kb on + strand, within MMP_RS01640 at 304.284 kb on + strand, within MMP_RS01640 at 304.313 kb on - strand, within MMP_RS01640 at 304.493 kb on - strand, within MMP_RS01640 at 304.773 kb on + strand, within MMP_RS01640 at 304.880 kb on - strand, within MMP_RS01640 at 304.943 kb on - strand at 304.943 kb on - strand at 304.975 kb on - strand at 305.071 kb on - strand at 305.078 kb on + strand at 305.096 kb on + strand at 305.097 kb on - strand at 305.097 kb on - strand at 305.097 kb on - strand at 305.098 kb on + strand at 305.139 kb on - strand at 305.139 kb on - strand at 305.139 kb on - strand at 305.139 kb on - strand at 305.140 kb on + strand at 305.140 kb on + strand at 305.140 kb on + strand at 305.140 kb on + strand at 305.141 kb on - strand at 305.142 kb on + strand at 305.142 kb on + strand at 305.142 kb on + strand at 305.380 kb on + strand, within MMP_RS01645 at 305.542 kb on + strand, within MMP_RS01645 at 305.625 kb on - strand, within MMP_RS01645 at 305.630 kb on - strand, within MMP_RS01645 at 305.773 kb on - strand at 305.792 kb on - strand at 305.792 kb on - strand at 305.884 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas no stress control 2 remove 302,408 - -0.3 302,447 - +1.0 302,455 + +1.8 302,459 + +0.3 302,558 + -1.1 302,702 + MMP_RS01635 0.26 +1.4 302,748 - MMP_RS01635 0.32 -1.0 302,755 + MMP_RS01635 0.33 +1.1 302,958 + MMP_RS01635 0.56 -0.8 302,966 + MMP_RS01635 0.57 +0.2 302,966 + MMP_RS01635 0.57 +0.4 302,985 - MMP_RS01635 0.59 -4.2 303,047 + MMP_RS01635 0.66 -0.5 303,301 + -1.1 303,449 - +2.0 303,612 - MMP_RS01640 0.16 -2.4 304,001 + MMP_RS01640 0.39 +1.6 304,051 + MMP_RS01640 0.41 +0.2 304,052 - MMP_RS01640 0.42 +0.3 304,092 + MMP_RS01640 0.44 +2.4 304,160 - MMP_RS01640 0.48 +1.2 304,163 - MMP_RS01640 0.48 +2.2 304,284 + MMP_RS01640 0.55 -1.0 304,284 + MMP_RS01640 0.55 -1.4 304,313 - MMP_RS01640 0.57 +1.1 304,493 - MMP_RS01640 0.67 -2.9 304,773 + MMP_RS01640 0.84 +1.6 304,880 - MMP_RS01640 0.90 +1.1 304,943 - +0.5 304,943 - -0.8 304,975 - +1.1 305,071 - -1.7 305,078 + +0.4 305,096 + -2.2 305,097 - -0.3 305,097 - +1.3 305,097 - -0.1 305,098 + -0.1 305,139 - +0.1 305,139 - -2.1 305,139 - -0.8 305,139 - +2.1 305,140 + -3.0 305,140 + +1.7 305,140 + +1.9 305,140 + +0.2 305,141 - -0.6 305,142 + -1.2 305,142 + -0.8 305,142 + +0.1 305,380 + MMP_RS01645 0.37 -2.5 305,542 + MMP_RS01645 0.60 +1.0 305,625 - MMP_RS01645 0.72 +1.4 305,630 - MMP_RS01645 0.73 -1.0 305,773 - -0.9 305,792 - -0.1 305,792 - +1.0 305,884 + +0.1
Or see this region's nucleotide sequence